Simultaneous Saccharification and Ethanol Fermentation of Corn Stover at High Temperature and High Solids Loading by a Thermotolerant Strain Saccharomyces cerevisiae DQ1

Chu, Deqiang; Zhang, Jian; Bao, Jie

doi:10.1007/s12155-012-9219-x

Cited by 37 publications

(23 citation statements)

References 19 publications

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“…Chu et al [9] used prehydrolysis at 50 °C for 12 h prior to ethanol production by SSF. Su et al [5] also used prehydrolysis at 50 °C for 24 h before ethanol production by simultaneous saccharification and co-fermentation (SSCF).…”

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

“…Although it could give high ethanol concentration over the benchmark level (above 4.0% (v/v)) [5,16,17], it might be economically viable to produce in large scale. However, almost of material pretreatment was used in high severity condition, especially high temperature, such as Chu et al [9] pretreated corn stover at 190 °C for 3 min, Zhu et al [15] pretreated aspen at 170 °C for 10 min, and Albuquerque-Wanderley et al [18] pretreated sugarcane bagasse at 200 °C for 7 min. In addition, the mixing with higher efficiency than incubator shaker was used, such as Chu et al [9] used helical stirring for mixing and fermented by thermotolerant yeast strain S. cerevisiae DQ1 at 40 °C.…”

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

“…However, almost of material pretreatment was used in high severity condition, especially high temperature, such as Chu et al [9] pretreated corn stover at 190 °C for 3 min, Zhu et al [15] pretreated aspen at 170 °C for 10 min, and Albuquerque-Wanderley et al [18] pretreated sugarcane bagasse at 200 °C for 7 min. In addition, the mixing with higher efficiency than incubator shaker was used, such as Chu et al [9] used helical stirring for mixing and fermented by thermotolerant yeast strain S. cerevisiae DQ1 at 40 °C. Ethanol production by FBP-SSF at 20% solid could produce ethanol of 35.76 g/L or 4.6% (v/v), which was close to benchmark level (above 4.0% (v/v)).…”

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

“…Whereas, unsuitable temperature of cellulase enzymatic hydrolysis will supplement the difficulty of hydrolysing substrate to fermentable sugar. To overcome these problems, many researchers used the SSF in fed-batch mode to maintain the low level of viscosity in slurry [2], screened and induced yeast to fermentation at high temperature, and used genitic engineering to thermo-tolerant yeast [5,9,10].…”

Section: Introduction mentioning

confidence: 99%

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Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Srinorakutara¹,

Subkaree²,

Boonvitthya³

et al. 2015

JFSE

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Effect of commercial cellulose enzymes was investigated by batch enzymatic hydrolysis at 15.0% (w/v) solid. It was found that the best commercial cellulose enzyme was Cellic ® CTec comparing to Accellerase 1000 TM and Accelerase 1500 TM . The Cellic ® CTec gave the highest reducing sugar concentration and rice straw conversion. Moreover, when the hydrolysate obtained from hydrolysis using Cellic ® CTec was fermented by Saccharomyces cerevisiae TISTR 5596, it would give the highest ethanol. In this study, the Cellic ® CTec was used for fed-batch prehydrolysis prior to ethanol production by simultaneous saccharification and fermentation (SSF) way at 20% (w/v) solid loading. It could produce 35.76 g/L or 4.6% (v/v) of ethanol concentration and 83.67 L/ton dry matter (DM) of yield.

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Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

Section: Introduction mentioning

confidence: 99%

See 2 more Smart Citations

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Srinorakutara¹,

Subkaree²,

Boonvitthya³

et al. 2015

JFSE

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“…Saccharomyces cerevisiae DQ1 (stored at Chinese General Microorganisms Collection Center; registration number: CGMCC 2528) was used as the ethanol fermenting strain [22,26]. S. cerevisiae DQ1 was first cultured in the synthetic medium (20 g/L glucose, 2 g/L KH 2 PO 4 , 1 g/L (NH 4 ) 2 SO 4 , 1 g/L MgSO 4 · 7H 2 O, 1 g/L yeast extracts) for activation and transferred to the same medium without glucose containing the corn stover hydrolysate for adaption according to the procedure described by Zhang et al [22].…”

Section: Strains and Enzymementioning

confidence: 99%

Helically Agitated Mixing in Dry Dilute Acid Pretreatment Enhances the Bioconversion of Corn Stover Into Ethanol

He¹,

Zhang²,

Zhang³

et al. 2015

Fuel Production From Non-Food Biomass

Self Cite

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Background: Dry dilute acid pretreatment at extremely high solids loading of lignocellulose materials demonstrated promising advantages of no waste water generation, less sugar loss, and low steam consumption while maintaining high hydrolysis yield. However, the routine pretreatment reactor without mixing apparatus was found not suitable for dry pretreatment operation because of poor mixing and mass transfer. In this study, helically agitated mixing was introduced into the dry dilute acid pretreatment of corn stover and its effect on pretreatment efficiency, inhibitor generation, sugar production, and bioconversion efficiency through simultaneous saccharification and ethanol fermentation (SSF) were evaluated. Results: The overall cellulose conversion taking account of cellulose loss in pretreatment was used to evaluate the efficiency of pretreatment. The two-phase computational fluid dynamics (CFD) model on dry pretreatment was established and applied to analyze the mixing mechanism. The results showed that the pretreatment efficiency was significantly improved and the inhibitor generation was reduced by the helically agitated mixing, compared to the dry pretreatment without mixing: the ethanol titer and yield from cellulose in the SSF reached 56.20 g/L and 69.43% at the 30% solids loading and 15 FPU/DM cellulase dosage, respectively, corresponding to a 26.5% increase in ethanol titer and 17.2% increase in ethanol yield at the same fermentation conditions. Conclusions: The advantage of helically agitated mixing may provide a prototype of dry dilute acid pretreatment processing for future commercial-scale production of cellulosic ethanol.

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Fermentation I – Microorganisms

Amarasekara¹

2013

Handbook of Cellulosic Ethanol

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Simultaneous Saccharification and Ethanol Fermentation of Corn Stover at High Temperature and High Solids Loading by a Thermotolerant Strain Saccharomyces cerevisiae DQ1

Cited by 37 publications

References 19 publications

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Helically Agitated Mixing in Dry Dilute Acid Pretreatment Enhances the Bioconversion of Corn Stover Into Ethanol

Fermentation I – Microorganisms

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