Simultaneous quantitative LC–ESI-MS/MS analyses of salicylic acid and jasmonic acid in crude extracts of Cucumis sativus under biotic stress

Segarra, Guillem; Jáuregui, Olga; Casanova, Eva; Trillas, Isabel

doi:10.1016/j.phytochem.2005.11.017

Cited by 139 publications

(97 citation statements)

References 27 publications

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“…Further SA identity confirmation was performed using UPLC-MS/MS (El Hadrami and Daayf, 2009), and quantification was performed using HPLC. JA was extracted and analyzed using UPLC-MS/MS as described previously (Segarra et al, 2006).…”

Section: Sa and Ja Quantificationsmentioning

confidence: 99%

Botrytis cinerea Manipulates the Antagonistic Effects between Immune Pathways to Promote Disease Development in Tomato

et al. 2011

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Plants have evolved sophisticated mechanisms to sense and respond to pathogen attacks. Resistance against necrotrophic pathogens generally requires the activation of the jasmonic acid (JA) signaling pathway, whereas the salicylic acid (SA) signaling pathway is mainly activated against biotrophic pathogens. SA can antagonize JA signaling and vice versa. Here, we report that the necrotrophic pathogen Botrytis cinerea exploits this antagonism as a strategy to cause disease development. We show that B. cinerea produces an exopolysaccharide, which acts as an elicitor of the SA pathway. In turn, the SA pathway antagonizes the JA signaling pathway, thereby allowing the fungus to develop its disease in tomato (Solanum lycopersicum). SA-promoted disease development occurs through Nonexpressed Pathogen Related1. We also show that the JA signaling pathway required for tomato resistance against B. cinerea is mediated by the systemin elicitor. These data highlight a new strategy used by B. cinerea to overcome the plant's defense system and to spread within the host.

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Section: Sa and Ja Quantificationsmentioning

confidence: 99%

Botrytis cinerea Manipulates the Antagonistic Effects between Immune Pathways to Promote Disease Development in Tomato

et al. 2011

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“…Total and free SA were quantified as described (Segarra et al, 2006;Garcion et al, 2008). Leaf tissues (250 mg), together with an internal standard (1 mg of o-anisic acid), were ground in liquid nitrogen and extracted twice with 90% methanol.…”

Section: Sa Quantificationmentioning

confidence: 99%

GDSL LIPASE1 Modulates Plant Immunity through Feedback Regulation of Ethylene Signaling

Kim

Kwon²,

Jang³

et al. 2013

Plant Physiology

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Ethylene is a key signal in the regulation of plant defense responses. It is required for the expression and function of GDSL LIPASE1 (GLIP1) in Arabidopsis (Arabidopsis thaliana), which plays an important role in plant immunity. Here, we explore molecular mechanisms underlying the relationship between GLIP1 and ethylene signaling by an epistatic analysis of ethylene response mutants and GLIP1-overexpressing (35S:GLIP1) plants. We show that GLIP1 expression is regulated by ethylene signaling components and, further, that GLIP1 expression or application of petiole exudates from 35S:GLIP1 plants affects ethylene signaling both positively and negatively, leading to ETHYLENE RESPONSE FACTOR1 activation and ETHYLENE INSENSITIVE3 (EIN3) down-regulation, respectively. Additionally, 35S:GLIP1 plants or their exudates increase the expression of the salicylic acid biosynthesis gene SALICYLIC ACID INDUCTION-DEFICIENT2, known to be inhibited by EIN3 and EIN3-LIKE1. These results suggest that GLIP1 regulates plant immunity through positive and negative feedback regulation of ethylene signaling, and this is mediated by its activity to accumulate a systemic signal(s) in the phloem. We propose a model explaining how GLIP1 regulates the fine-tuning of ethylene signaling and ethylene-salicylic acid cross talk.

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“…A signal is detected only when the selected precursor ion passes the first-stage quadrupole and the selected product ion passes the third-stage quadrupole; thus each ionized compound gives a distinct precursor-to-product ion transition in the MRM mode that is diagnostic for the presence of a particular compound in an extract., Figure 1 gives the principle of isotope dilution using MRM mode on triple quadrupole mass spectrometer. The MRM mode shows higher selectivity and sensitivity than SIM and can detect the target plant hormones quickly [79,80]. Highly sensitive MS techniques have greatly reduced the amount of sample tissues required.…”

Section: Chromatography and Chromatography/mass Spectrometrymentioning

confidence: 99%

Progress in quantitative analysis of plant hormones

Sun

Wang

et al. 2011

Chin. Sci. Bull.

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Plant hormones are small molecular natural products that regulate all plant developmental processes at low concentrations. Quantitative analysis of plant hormones is increasingly important for in-depth study of their biosynthesis, transport, metabolism and molecular regulatory mechanisms. Although plant hormone analysis remains a bottleneck in plant scientific research owing to the trace concentrations and complex components in plant crude extracts, much progress has been achieved in the development of extraction, purification and detection techniques in recent years. Solid phase extraction and chromatography/mass spectrometry have been applied widely for purification and quantitative analysis of plant hormones owing to their high selectivity and sensitivity. Purification methods such as liquid partition and immunoaffinity chromatography, and detection methods including immunoassay and electrochemical analysis, are employed. The advantages and disadvantages of these methods are discussed. In situ, real-time and multi-plant hormone profiling will comprise mainstream techniques for quantitative analyses in future studies on the regulatory mechanisms and crosstalk of plant hormones. defense responses are all triggered by plant hormones. A plant hormone, acting as a plant-development controller or a plant-environment mediator, can influence crop yield directly or indirectly, therefore research into plant hormone physiology has become an important target for agriculture development. However, it is noteworthy that endogenous level of each plant hormone is very low [1], and quantitative analysis of these compounds is still extremely difficult for both biologists and analytical chemists. It is a challenge for researchers to enrich these low-level natural compounds from plant extracts in which large amounts of interfering substances are present. In addition, the limited quantities of plant tissue available for hormone quantification is another challenge. Consequently, the analytic methods must be extremely selective and sensitive. The quantitative analysis of plant hormones typically includes the following key points: (i) the plant materials must

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Simultaneous quantitative LC–ESI-MS/MS analyses of salicylic acid and jasmonic acid in crude extracts of Cucumis sativus under biotic stress

Cited by 139 publications

References 27 publications

Botrytis cinerea Manipulates the Antagonistic Effects between Immune Pathways to Promote Disease Development in Tomato

Botrytis cinerea Manipulates the Antagonistic Effects between Immune Pathways to Promote Disease Development in Tomato

GDSL LIPASE1 Modulates Plant Immunity through Feedback Regulation of Ethylene Signaling

Progress in quantitative analysis of plant hormones

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