Simultaneous Quantitative Determination of Imidazole Antimycotics in Human Urine by using RPLC Technique

Abstract

Imidazoles are well known heterocyclic compounds having important feature of a variety of medicinal agents. Studies with imidazole containing compounds are great importance in the clinical use of drugs. The presence of imidazole group in the molecules of lanoconazole (LCZ) and butoconazole (BCZ) is very important for its chemical and analytical behavior. Target analytes were efficiently separated with a Pinnacle DB Cyano column (5 µm, 4.6 x 250 mm I.D.) and oxiconazole (OCZ) used as an internal standard. In this study, correlation between the retention factors of the solutes and pH of the mobile phase was determined at ten pH values in acetonitrile-water binary mixture. In optimization condition, quantification of these drugs were performed in human urine. The linear responses were observed over a wide concentration range (6.25.

Reverse Phase Liquid Chromatography Method Optimization for Analysis of Some Azole Group Antifungal Compounds

“…Bu yaygın kullanılan antifungal ajanların kalitatif ve kantitatif tayini için en yaygın kullanılan analitik teknik yüksek performans sıvı kromatografidir. Bu teknik içerisinde bu grubun analizi için en çok ters faz sıvı kromatografi (RPLC) metodu tercih edilmektedir [5][6][7][8][9]. RPLC metot optimizasyonunda en önemli amaç en iyi ayırma koşullarını sağlamaktır.…”

N-Methylfulleropyrrolidine-Based Multimode Sensor for Determination of Butoconazole Nitrate

“…5 A literature survey revealed several other methods proposed for the determination of BTC from various matrices such as pure form, human plasma and urine, rat plasma and tissue, cream, and water samples. These methods include ultraperformance liquid chromatography (UPLC) with UV detection, 6 reversed-phase liquid chromatography (RPLC) with UV detection, 7 high-performance thin-layer chromatography (HPTLC)-densitometry, 8 fabric phase sorptive extraction-HPLC with photodiode array detection (FPSE-HPLC-PDA), 9 microextraction by packed sorbent-HPLC (MEPS-HPLC-DAD), 10 liquid chromatography-tandem mass spectrometry (LC-MS/MS), 11 , 12 capillary electrophoresis (CE), 13 and spectrophotometry. 14 Since BTC is a chiral molecule, liquid chromatography-electrospray ionization coupled with tandem mass spectrometry (LC-ESI-MS/MS) was proposed to detect its enantiomers.…”