Simultaneous Quantitative Analysis of Shikonin and Deoxyshikonin in Rat Plasma by Rapid LC–ESI–MS–MS

Huang, Sufang; Yu, Lanfang; Shen, Peng

doi:10.1365/s10337-010-1599-5

Cited by 16 publications

(10 citation statements)

References 23 publications

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“…Data were collected in negative ion mode using an extended dynamic range (2 GHz). Additional LC/MS-MS experimentation was performed for selected molecules of interest ( Huang et al , 2010 ) using analytical standards of acetylshikonin (MW 330.1103; RT 5.75 min±0.7min), deoxyshikonin (MW 272.1048; RT 6.75±0.7min), dimethylacrylshikonin (MW 370.1416; RT 7.7 min±0.7min) purchased from ChemFaces (Wuhan, China) and shikonin (MW 288.0997; RT 3.51 min±0.7min) obtained from Biomol (Hamburg, Germany). All data were analysed using MassHunter software (ver.…”

Section: Methodsmentioning

confidence: 99%

Identification and localization of bioactive naphthoquinones in the roots and rhizosphere of Paterson’s curse (Echium plantagineum), a noxious invader

Zhu

Skoneczny

Weidenhamer

et al. 2016

EXBOTJ

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Section: Methodsmentioning

confidence: 99%

Identification and localization of bioactive naphthoquinones in the roots and rhizosphere of Paterson’s curse (Echium plantagineum), a noxious invader

Zhu

Skoneczny

Weidenhamer

et al. 2016

EXBOTJ

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“…To verify this possible molecular binding activity, an antibody pull-down assay in combination with LC-MS/MS analysis was employed to determine the intracellular interaction between SK and hnRNPA1 protein. The chromatographic and mass spectrometric conditions for detection of SK in this assay were optimized from a previous report [28]. The production spectrum of an SK standard (Figure 1a) showed a significant precursor ion signal (m/z = 287.0921) and the MS/MS fragmental spectra for its precursor showed a major fragment (m/z = 218.0226).…”

Section: Resultsmentioning

confidence: 99%

“…The workflow used for detecting SK using LC-MS/MS has been previously described [28]. In this study, the LC-MS/MS analysis performance was further optimized by coupling an ultrahigh performance liquid chromatography (UPLC) system (ACQUITY UPLC, Waters, Millford, MA) to an ultrahigh resolution tandem mass spectrometer (LTQ Orbitrap Elite, Thermo Fisher Scientific, Bremen, Germany).…”

Section: Methodsmentioning

confidence: 99%

Immunogenicity of mammary tumor cells can be induced by shikonin via direct binding-interference with hnRNPA1

et al. 2016

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Immunogenic cell death (ICD) of tumor cells occurs via various pathways that activate immune cell systems against cancer. Previous studies have demonstrated that shikonin (SK), a plant secondary metabolite, can confer strong pharmacological activities that activate ICD and strong immunogenicity of tumor cells. However, the exact hierarchical regulatory mechanisms including the molecular targets of SK-activated immunogenicity are still unknown. Here, the heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) was revealed to serve as a specific protein target for SK. This binding plays a key role in SK-stimulated ICD activity and the suppression of post-transcriptional mRNA processing, including nuclear export activity of newly synthesized mRNAs in mammary carcinoma cells in vitro. Moreover, it also mechanistically mediates the anti-metastatic effect of a tumor cell lysate (TCL) vaccine, which can be readily generated from SK-treated 4T1 tumor cells (SK-TCL), and the derived tumor-immunogenicity of SK-TCL-treated dendritic cells in vivo. Together, the identification of hnRNPA1 as the intracellular molecular target provides compelling pharmacology-based knowledge for the potential clinical use of SK-induced immunogenicity. In addition, SK may also serve as a potent suppressor that interferes with specific post-transcriptional activities, a mechanism which may be useful for exploitation in cancer therapeutics.

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“…The method was validated according to the literature [11] and FDA guideline [12]. Validation runs were conducted on three consecutive days.…”

Section: Validation Of the Methodsmentioning

confidence: 99%

LC–APCI–MS Quantitative Analysis of Curcumin in Rabbit Plasma

Yang

et al. 2011

Chromatographia

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A simple and specific liquidchromatographyatmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS) method was developed and validated for quantitative analysis of curcumin in rabbit plasma. Chromatographic separation was carried out on Zorbax SB-C18 column, acetonitrile-methanol-water used as mobile phase with gradient elution. APCI source was applied and operated in negative ion mode, and selective ion monitoring mode used to quantify curcumin. The method showed excellent intra-assay and inter-assay precision (relative standard deviations and bias\15%) for quality control (QC) samples. This method was successfully applied to a pharmacokinetic study of curcumin after intravenous administration to rabbits.

show abstract

Simultaneous Quantitative Analysis of Shikonin and Deoxyshikonin in Rat Plasma by Rapid LC–ESI–MS–MS

Cited by 16 publications

References 23 publications

Identification and localization of bioactive naphthoquinones in the roots and rhizosphere of Paterson’s curse (Echium plantagineum), a noxious invader

Identification and localization of bioactive naphthoquinones in the roots and rhizosphere of Paterson’s curse (Echium plantagineum), a noxious invader

Immunogenicity of mammary tumor cells can be induced by shikonin via direct binding-interference with hnRNPA1

LC–APCI–MS Quantitative Analysis of Curcumin in Rabbit Plasma

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