LC–APCI–MS Quantitative Analysis of Curcumin in Rabbit Plasma

Abstract

A simple and specific liquidchromatographyatmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS) method was developed and validated for quantitative analysis of curcumin in rabbit plasma. Chromatographic separation was carried out on Zorbax SB-C18 column, acetonitrile-methanol-water used as mobile phase with gradient elution. APCI source was applied and operated in negative ion mode, and selective ion monitoring mode used to quantify curcumin. The method showed excellent intra-assay and inter-assay precision (relative standard deviations and bias\15%) for quality control (QC) samples. This method was successfully applied to a pharmacokinetic study of curcumin after intravenous administration to rabbits.

Application of a New Validated HPLC-PDA Method for Simultaneous Determination of Curcumin and Melatonin in Hyaluronic Acid-Coated Nanoemulsions

“…30,31 Literature on analysis of CUR revealed several methods based on different techniques, such as high-performance liquid chromatography (HPLC) with fluorescence detection, which have been used for its quantification in biological samples, and liquid chromatography-mass spectroscopy (LC-MS) for analysis in food products and plasma. [32][33][34] HPLC methods with UV detection were also used for determining CUR in biological fluids, liquid crystals, tablets and capsules, and liposomes. 8,[35][36][37] Similarly, MEL determination in biological and pharmaceutical samples, such as nanoparticles, have been carried out by HPLC with fluorescence and UV detection.…”

Development of UPLC–MS/MS method for quantitative analysis of curcumin in human plasma

“…Nowdays, many formulations of curcumin have been reported to enhance its bioavailability [7][8][9]; therefore, the sensitive, specific, and validated analytical method for the compound in human plasma is essential to prove the effectiveness of such formulations. Several methods for determination of the compound in biological samples by high-performance liquid chromatography (HPLC) including HPLC-tandem mass spectrometry (MS/MS) have been developed, but there are only few reports on the determination of the compound by ultra-performance liquid chromatography (UPLC)-MS/MS [9][10][11][12][13][14][15][16][17][18]. The UPLC-MS/MS is currently considered as the best choice for supporting bioanalytical studies due to high specificity, sensitivity, and rapidity [19]; however, there has been no report that the method was developed for the determination of curcumin in human plasma [9,18].…”