Simultaneous quantitation of nucleoside HIV-1 reverse transcriptase inhibitors by short-end injection capillary electrochromatography on a β-cyclodextrin-bonded silica stationary phase

Mesplet, Nathalie; Morin, Pascal; François, C.; Agrofoglio, Luigi A.

doi:10.1016/s0021-9673(01)01063-9

Cited by 30 publications

(25 citation statements)

References 23 publications

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“…Sol-gel materials [9,10] and organic polymer continuous beds [11,12] are also under development and there has also been interest in the use of vancomycin [13,14], cellulose/ amylase [15 18], or teicoplanin [19,20] stationary phases. Although fl-cyclodextrin-bonded materials have been used for separation of enantiomers [21 23] they have rarely been employed for achiral separations [24].…”

Section: Capillary Electrochromatographymentioning

confidence: 99%

An original way to use a β-cyclodextrin-bonded silica stationary phase in electrochromatography. Application to the achiral separation of nucleobases and nucleosides

et al. 2002

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Key WardsCapillary electrochromatog raphy ,8-Cyclodextrin-bonded silica Purines Pyrimidines and nucleosides SummaryAlthough/~-cyclodextrin-bonded silica stationary phases are usually dedicated to the separation of enantiomers, they can also be used for achiral purposes, taking advantage of the complex interactions be~een the solutes and the cyclodextrins. The work described in this paper was intended to show how such stationary phases can be used in short-end injection capillary electrochromatography (CEC) for rapid (< 6 min) resolution of a mixture of purines and pyrimidines. Several experimental conditions (mobile-phase composition, voltage and temperature) were investigated to determine the optimum operating conditions for the method, which can also be applied to the analysis of nucleosides. Quantitative analysis was also performed; theophylhne was used as internal standard to assess the linearity and the repeatability of the method. Limits of detection ranged from 4 to 13 I~M and repeatability was good (RSD never exceeded 2.6%).

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Section: Capillary Electrochromatographymentioning

confidence: 99%

An original way to use a β-cyclodextrin-bonded silica stationary phase in electrochromatography. Application to the achiral separation of nucleobases and nucleosides

et al. 2002

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“…Mesplet et al [80,81] developed a CEC method for the analysis of nucleoside HIV reverse transcriptase inhibitors (NRTIs) using a b-CD-bonded silica stationary phase. In order to decrease analysis time, CEC was carried out on a packed capillary with 10 cm of effective length.…”

Section: Nucleosides and Nucleotidesmentioning

confidence: 99%

Recent applications in capillary electrochromatography

2003

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The most recent and important applications in capillary electrochromatography (CEC) are summarized, covering literature published since May 2001. A selection of new developments in stationary phases for CEC is highlighted, and enantiomeric separations and chiral stationary phases are discussed. Also, CEC applications of biological molecules, pharmaceuticals, and applications in the field of industrial and environmental analysis are summarized. For this review three modes of CEC were taken into account, i.e., packed-column CEC, CEC using monolith technology, and open-tubular CEC.

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“…Mesplet et al [43,44] separated and quanti®ed the nucleoside HIV reverse transcriptase inhibitors by short-end injection capillary electrochromatography on a b-cyclodextrin-bonded silica stationary phase, proving that CEC would be a suitable tool for both the quantitative determination and the assay of a mixture of nucleosides.…”

Section: Capillary Electrochromatography Of Nucleosides and Nucleotidesmentioning

confidence: 99%

The separation of biomolecules using capillary electrochromatography

Huang

Jin

et al. 2003

Current Opinion in Biotechnology

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Simultaneous quantitation of nucleoside HIV-1 reverse transcriptase inhibitors by short-end injection capillary electrochromatography on a β-cyclodextrin-bonded silica stationary phase

Cited by 30 publications

References 23 publications

An original way to use a β-cyclodextrin-bonded silica stationary phase in electrochromatography. Application to the achiral separation of nucleobases and nucleosides

An original way to use a β-cyclodextrin-bonded silica stationary phase in electrochromatography. Application to the achiral separation of nucleobases and nucleosides

Recent applications in capillary electrochromatography

The separation of biomolecules using capillary electrochromatography

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