Simultaneous quantitation of carbohydrate antigen 125 and carcinoembryonic antigen in human serum via time-resolved fluoroimmunoassay

Huang, Zhen; Zhai, Xiang-Ming; Wang, Hao; Deng, Qiaoting; Li, Kun; Liu, Bi-Sen; Wang, Gang; Liu, Tiancai

doi:10.1016/j.cca.2018.05.003

Cited by 20 publications

(10 citation statements)

References 29 publications

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“…From other researches, CA125 was associated with R0 resection rate [13], recurrence and peritoneal dissemination [14] and OS in the unresectable advanced or recurrent GC patients [15]; CA199 was related to pN[7, [16][17][18] and pTNM stage [17], and CA724 possessed the larger area under the receiver operating curve, which meant a higher diagnostic efficiency [19]. However, none of their patients underwent NCT.…”

Section: Discussionmentioning

confidence: 99%

CA724 predicts overall survival in locally advanced gastric cancer patients with neoadjuvant chemotherapy

Tong

Zhao

Shan

et al. 2020

Preprint

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Background: Serum tumor markers are of great importance in diagnosis, prognostic predicting and recurrence monitoring in gastrointestinal malignancy, including AFU, AFP, CEA, CA199, CA125 and CA724. However, their significances in gastric cancer (GC) patients with neoadjuvant therapy (NCT) are still uncertain. The aim of this study is to evaluate the predictive value of these six tumor markers in locally advanced GC patients with NCT and curative surgery. Methods: 290 locally advanced GC patients with NCT and D2 radical gastrectomy were retrospectively analyzed. Their tumor markers before (pre-) and after (post-) NCT and pathological characters were exacted from the database in our hospital. The optimal cutoff values of six tumor markers were calculated by ROC and Youden index. Their predictive significances were analyzed and survival curves on overall survival (OS) were obtained by Kaplan-Meier method. Associations between categorical variables were explored by Chi-square test or Fisher's exact method. Multivariate analyses were performed by Cox regression model. Results: Not only the pre- and post- CA199, CA125 and CA724 could predict the OS respectively, but also the changes (diff-) between post- and pre- groups were related to the prognosis (P < 0.05). In multivariable analysis, only pre- (P = 0.016) and post-CA724 (P = 0.033) remained significant, and the significance of diff-CA724 was on borderline (P = 0.085). Besides, pre- and post-CA199, CA125 and CA724 were associated with the metastasis of lymph node (N- vs N+) and pathological stage (Ⅰ-Ⅱ vs Ⅲ) (P < 0.05). Post-CA724 was related to the invasion of vascular or lymphatic vessels (P = 0.019), and pre-CA724 was nearly remarkable (P = 0.082). However, AFU, AFP and CEA showed no association with survival (P > 0.05). Conclusions: CA724 is an independent factor to prognosis, and could be used to predict the ypN and ypTNM stage in locally advanced GC patients undergone NCT and curative resection.

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Section: Discussionmentioning

confidence: 99%

CA724 predicts overall survival in locally advanced gastric cancer patients with neoadjuvant chemotherapy

Tong

Zhao

Shan

et al. 2020

Preprint

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“…Other studies have shown that, CA125 was associated with the R0 resection rate [13], recurrence, peritoneal dissemination [14] and OS in unresectable advanced or recurrent GC patients [15]; CA199 was related to pN [7,[16][17][18] and pTNM stage [17]; and CA724 was related to the pathological stage and had a good diagnostic value for gastric cancer [19]. However, none of the patients in those studies underwent NCT.…”

Section: Discussionmentioning

confidence: 95%

CA724 predicts overall survival in locally advanced gastric cancer patients with neoadjuvant chemotherapy

Tong

Zhao

Shan

et al. 2020

Preprint

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Background: Serum tumor markers including AFU, AFP, CEA, CA199, CA125 and CA724, are of great importance in the diagnosis, prognostic prediction and recurrence monitoring of gastrointestinal malignancies. However, their significance in gastric cancer (GC) patients with neoadjuvant therapy (NCT) is still uncertain. The aim of this study was to evaluate the predictive value of these six tumor markers in locally advanced GC patients who underwent NCT and curative surgery. Methods: In total, 290 locally advanced GC patients who underwent NCT and D2 radical gastrectomy were retrospectively analyzed. Data on their tumor markers before (pre-) and after (post-) NCT and pathological characteristics were extracted from the database of our hospital. The optimal cutoff values of the six tumor markers were calculated by the ROC curve and Youden index. Their predictive significance was analyzed and survival curves for overall survival (OS) were obtained by the Kaplan-Meier method. Associations between categorical variables were explored by the chi-square test or Fisher's exact test. Multivariate analyses were performed by the Cox regression model. Results: Pre- and post-CA199, -CA125 and -CA724 could predict overall survival (all P < 0.05), but only the change (diff-) of CA199 was related to prognosis (P = 0.05). In the multivariable analysis, pre- (P = 0.014) and post-CA724 (P = 0.036) remained significant, though diff-CA724 was not an independent prognostic factor (P = 0.581). In addition, pre- and post-CA199, -CA125 and -CA724 were associated with lymph node metastasis (N- vs N+) and pathological stage (Ⅰ-Ⅱ vs Ⅲ) (all P < 0.05). Moreover, post-CA724 was related to the vascular or lymphatic invasion (P = 0.019), while pre-CA724 was not (P = 0.082). However, AFU, AFP and CEA showed no association with survival (P > 0.05). Conclusions: CA724 is an independent factor for prognosis and could be used to predict ypN and ypTNM stage in locally advanced GC patients undergoing NCT and curative resection.

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“…Planar multiplex electrochemiluminescence sandwich assays immobilize multiple analyte-specific capture antibodies to a solid matrix (slide or microtiter plate well). Bound target cytokines from samples are then recognized by detection antibodies coupled with electrochemiluminescence reporters that, when electrically stimulated, emit light whose wavelength and intensity correlates to the concentration of a specific analyte (6,23).…”

Section: Discussionmentioning

confidence: 99%

“…This is highlighted by the steadily increasing number of multiplex proteomic assays that receive FDA approval for clinical application (4). Identifying disease-specific biomarker signatures in human plasma may be useful in diagnosing diverse health disorders including neoplastic, cardiovascular, pulmonary, metabolic, autoimmune, neurodegenerative, and infection/sepsis pathologies, among many others (5)(6)(7)(8)(9)(10)(11)(12). This approach may be valuable in deciding best treatment options, tracking disease progression and response to therapy, and formulating prognoses (13).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Bead-Based Fluorescence Versus Planar Electrochemiluminescence Multiplex Immunoassays for Measuring Cytokines in Human Plasma

et al. 2020

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This study compared two 96-well multiplex immunoassay platforms for analytical performance in assessing cytokine concentrations in standards, quality controls and human plasma samples (n = 62), and evaluated assay time requirements. Assays included a bead-based fluorescence MILLIPLEX R assay/Luminex fluorescence platform (LMX) and three kits from Meso Scale Discovery (MSD) in planar electrochemiluminescence format. The LMX kit evaluated 21 cytokines and the MSD kits evaluated 10 cytokines each, with 16 overlapping cytokines between platforms. Both assays provided good reproducibility in standard curves for all analytes. Interassay CVs of shared analytes showed average kit quality control CVs ranging 1.9-18.2% for LMX and 2.4-13.9% for MSD. The MSD platform had lower LLoQs than LMX for 14/16 shared cytokines. For IL-17, the LLoQ was lower with LMX than MSD, and the LLoQs for IL-6 were similar. Although MSD calibration curves indicated lower LLoQs for most of those analytes, many more cytokines in human plasma samples were not detected by MSD than by LMX. The ULoQs were higher in LMX versus MSD assays for 13/16 shared analytes, lower than MSD for IL-17, and equivalent between assays for IL-6 and MIP-1α. Bland-Altman plots indicated that MSD classified 13/16 shared analytes as concentrations lower than by LMX. Time and motion analysis indicated that total mean assay times were 20 h 28 m and 21 h 33 m for LMX and MSD, respectively, including an overnight (17 h) incubation. The MSD assays employed a manufacturer-approved overnight incubation instead of the standard 2-h incubation, which kit instructions suggest might increase detection sensitivity. Hands-on labor time averaged 1 h 37 m for LMX and 2 h 33 m for MSD. In summary, assay selection factors should include selection of specific markers of interest, time and cost considerations, and anticipated cytokine concentrations in prospective samples.

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Simultaneous quantitation of carbohydrate antigen 125 and carcinoembryonic antigen in human serum via time-resolved fluoroimmunoassay

Cited by 20 publications

References 29 publications

CA724 predicts overall survival in locally advanced gastric cancer patients with neoadjuvant chemotherapy

CA724 predicts overall survival in locally advanced gastric cancer patients with neoadjuvant chemotherapy

CA724 predicts overall survival in locally advanced gastric cancer patients with neoadjuvant chemotherapy

Comparison of Bead-Based Fluorescence Versus Planar Electrochemiluminescence Multiplex Immunoassays for Measuring Cytokines in Human Plasma

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