Simultaneous quantification of ondansetron and tariquidar in rat and human plasma using a high performance liquid chromatography‐ultraviolet method

Abstract

Ondansetron, a widely used antiemetic agent, is a P‐glycoprotein (P‐gp) substrate and therefore expression of P‐gp at the blood–brain barrier limits its distribution to the central nervous system (CNS), which was observed to be reversed by coadministration with P‐gp inhibitors. Tariquidar is a potent and selective third‐generation P‐gp inhibitor, and coadministration with ondansetron has shown improved ondansetron distribution to the CNS. There is currently no reported bioanalytical method for simultaneously quantifying ondansetron with a third‐generation P‐gp inhibitor. Therefore, we aimed to develop and validate a method for ondansetron and tariquidar in rat and human plasma samples. A full validation was performed for both ondansetron and tariquidar, and sample stability was tested under various storage conditions. To demonstrate its utility, the method was applied to a preclinical pharmacokinetic study following coadministration of ondansetron and tariquidar in rats. The presented method will be valuable in pharmacokinetic studies of ondansetron and tariquidar in which simultaneous determination may be required. In addition, this is the first report of a bioanalytical method validated for quantification of tariquidar in plasma samples.

Quantification of vincristine and tariquidar by liquid chromatography‐tandem mass spectrometry in mouse whole blood using volumetric absorptive microsampling for pharmacokinetic applications

“…Validation parameters achieved in this study are comparable to currently available methods for the quantification of vincristine and tariquidar analytes. Most published studies utilize LC-MS/MS instrumentation for quantification of vincristine [13,[23][24][25][26][27][28][29]and tariquidar [30] over HPLC with ultraviolet detection [31][32][33] given its superior sensitivity. To date, this study has achieved the lowest collected mouse whole blood volume for accurate quantification of both analytes.…”

A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker

“…According to the literature [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24], acetonitrile and acidic water media were selected to optimize the mobile phase composition. A series of concentrations, pH values, the ratio of aqueous potassium dihydrogen phosphate solution, and different chromatographic column types were studied to ensure good resolution and appropriate retention time of the five 5-HT 3 receptor antagonists.…”

“…HPLC is commonly used to verify rapid detection systems due to its attractive features, such as high peak efficiencies, great resolution, high sensitivity, good repeatability, and wide application range. HPLC analytic methods for the detection of 5-HT 3 receptor antagonists such as ODT, GNT, TPT, AZT, and RMT are already mature, and most of them use retention data in the qualitative analysis, while the external standard method (ESM) is applicable in quantitative analysis [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. However, some of these methods may take a long time, and the specific drugs to be tested must correspond to reference substances, which cannot meet the requirements of rapid drug detection.…”

Application of the HPLC Method in Parenteral Nutrition Assessment: Stability Studies of Ondansetron

“…Several bioanalytical methods have been reported in the literature for the quantification of OND, for example, spectrophotometric [20] and more popular liquid chromatography with UV [21][22][23][24] or MS [25][26][27][28][29] detection. Raza et al [20] estimated drug concentration in bulk material using the spectrophotometry method.…”

“…Raza et al [20] estimated drug concentration in bulk material using the spectrophotometry method. Numerous papers described OND determination in biological matrixes [26,29], i.e., human plasma or animal tissues in rats [22,25], cats [27], and dogs [30]. Investigations on the determination of OND, together with other drugs in infusion fluids, constitute a separate part.…”