Simultaneous quantification of levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin in microvolumes of human plasma using high‐performance liquid chromatography with ultraviolet detection

Abstract

Levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin are four fluoroquinolones used in the treatment of serious bacterial infections. The antibacterial activity of fluoroquinolones is concentration dependent. Therefore, therapeutic drug monitoring in daily clinical practice is warranted to ensure the therapy's efficacy and prevent bacterial resistance. The purpose of the present study was to develop a method using high‐pressure liquid chromatography with an ultraviolet detector for simultaneous quantification of these four fluoroquinolones in human plasma. A 50 μL aliquot of plasma was precipitated by 200 μL of methanol using gatifloxacin as internal standard. The chromatographic separation was performed on a Kinetex XB‐C18 column using a mobile phase composed of a mixture of orthophosphoric acid 0.4% (v/v), acetonitrile and methanol at a flow rate of 1.2 mL/min. Dual UV wavelength mode was used, with levofloxacin and moxifloxacin monitored at 293 nm, and pefloxacin and ciprofloxacin monitored at 280 nm. The calibration was linear over the ranges of 0.125–25 mg/L for levofloxacin, 0.1–20mg/L for moxifloxacin and 0.05‐10 mg/L for both pefloxacin and ciprofloxacin. Inter‐ and intra‐day trueness and precision were <13% for all the compounds under study. The proposed method was simple, reliable, cost‐effective and suitable for therapeutic drug monitoring or pharmacokinetics studies.

A new HPLC–DAD method for contemporary quantification of 10 antibiotics for therapeutic drug monitoring of critically ill pediatric patients

“…To evaluate the linearity of this HPLC method, plasma calibration curves were determined in triplicate on three separate days. The correlation coefficients and the calibration ranges for each compound are reported in Table 1 The calibration ranges were defined in accordance with the expected plasma concentration in patients (McWhinney et al, 2010; Naicker et al, 2018; Xie et al, 2014; Zheng et al, 2019).…”

A Tb3+ functionalized triazine-porous organic framework as a ratiometric fluorescent sensor for determination of ciprofloxacin in aquatic products

“…A variety of methods have been used for the determination of the ciprofloxacin residue, for example, the capillary electrophoresis method, 8 high-performance liquid chromatography method, 9 and high-performance liquid chromatography-mass spectrometry method. 10 However, the application of these methods requires expensive equipment or heavy time-consumption.…”

Development and validation of a UPLC-MS/MS method for simultaneous quantification of levofloxacin, ciprofloxacin, moxifloxacin and rifampicin in human plasma: Application to the therapeutic drug monitoring in osteoarticular infections

“…The recommended therapeutic range for RIF corresponds to an AUC0-12 between 30 and 65 µg.h -1 .mL -1 , and a Cmax between 8 and 24 µg/mL [10,11]. Several analytical methods such as liquid chromatography coupled to ultraviolet (LC-UV) [12,13] or mass spectrometry (LC-MS/MS) [14][15][16][17][18][19] are reported for the analysis of LVX, CPX, MOX and RIF alone or in combination with other compounds; yet, the established methods suffer in some cases from the limited range of covered concentrations and/or from a long run time, which make them less suitable in clinical practice. The aim of this study was to establish a rapid, selective and simple LC-MS/MS method for simultaneous analysis of LVX, CPX, MOX and RIF suitable for routine analysis and pharmacokinetic studies.…”