A new HPLC–DAD method for contemporary quantification of 10 antibiotics for therapeutic drug monitoring of critically ill pediatric patients

Abstract

The common practice of therapeutic drug monitoring (TDM) involves the quantification of drug plasma concentrations at a specific time in a dosing window. Although TDM for antibiotics is not considered mandatory, it may represent a valid tool for clinicians in order to limit antibiotic resistance and avoid therapeutic failures. The aim of our study was to develop and validate a high‐performance liquid chromatography–diode array detection method for simultaneous quantification of 10 antibiotics in plasma. This method has a fast analytical procedure that uses the same chromatographic conditions to quantify ceftazidime, ceftriaxone, meropenem, ertapenem, ciprofloxacin, tigecycline, ampicillin, levofloxacin and piperacillin, plus the β‐lactamase inhibitor tazobactam. Method validation was ensured by testing selectivity, accuracy, precision, limits of detection and quantification, recovery and stability. The calibration ranges, established accordingly to the expected plasma concentration in patients, showed a coefficient of determination >0.996 for all compounds. Within‐ and between‐days precisions reported a coefficient of variation >15%. Similarly, the accuracy evaluation reported a relative standard deviation of <10% for each antibiotic. The recovery ranged between 97 and 103% for all compounds. This method could represent a useful tool for TDM of antibiotics.

Therapeutic Drug Monitoring Is a Feasible Tool to Personalize Drug Administration in Neonates Using New Techniques: An Overview on the Pharmacokinetics and Pharmacodynamics in Neonatal Age

“…As an example, in a comparative PK study on neonates, our group has successfully used microtubes to collect capillary blood samples using a heel stick, overcoming phlebotomy [38]. Furthermore, CMS is currently used in our institution for routine monitoring of antibiotics in critically ill neonates [39].…”

Synergistic Meropenem/Vaborbactam Plus Fosfomycin Treatment of KPC Producing K. pneumoniae Septic Thrombosis Unresponsive to Ceftazidime/Avibactam: From the Bench to the Bedside

“…Plasma was separated by centrifugation at 3500× g for 5 min and stored at 80 • C until analysis. The plasma levels of MEM were determined by high performance liquid chromatography (HPLC), the extraction procedure and HPLC analysis were performed as reported by Cairoli et al [25]. The plasma levels of vaborbactam and FOS were determined by liquid chromatography and mass spectrometry (UHPLC) Agilent 1290 Infinity II 6470 (Agilent Technologies, Waldbronn, Germany) equipped with an ESI-JET-STREAM source operating in the positive ion (ESI+) mode for FOS and in the negative ion (ESI−) mode for vaborbactam.…”

Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis

“…This paper describes how we developed our method for detecting ciprofloxacin (Cpx) and ofloxacin (Ofx) in animal tissues. Several HPLC-UV methods enable the determination of these compounds in various matrices, e.g., in human plasma and urine [2,7]. Methods based on HPLC-MS for FQLs' determination in wastewater [8,9] or animal products [10,11] are also described in the literature.…”