Simultaneous quantification of eleven bioactive phenolic compounds in pigeon pea natural resources and in vitro cultures by ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS)

Gai, Qing-Yan; Jiao, Jiao; Wang, Xin; Fu, Yujie; Lu, Yao; Liu, Jing; Wang, Ziying; Xu, Xiaojie

doi:10.1016/j.foodchem.2020.127602

Cited by 21 publications

(16 citation statements)

References 37 publications

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“…Fragment ions m / z 283 were generated as m / z 341continued to lose one molecule of H 2 O and CO. When compared with the standard information and literature [ 29 ], compound 10 was identified as vitexin.…”

Section: Resultsmentioning

confidence: 99%

Phenolics Profile and Protective Effect on Injuried HUVEC Cells of Epicarp Extracts from Kadsura coccinea

Yang

Cheng

et al. 2022

Foods

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This study evaluated the phenolics profile and the antioxidative properties of K. coccinea fruits epicarp. A total of 13 phenolic compounds (six phenolic acids, four anthocyanins, two flavonols, and one flavone) were identified by ultra performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spetrometry(UPLC-QTOF-MS/MS). Two anthocyanins, cyanidin-3-xylosylrutinoside and cyanidin-3-rutinoside, comprise 30.89~74.76% and 13.90~46.17% of the total amount of anthocyanins in K. Coccinea. Cytoprotective effect results evidenced that pretreatment of Human umbilical vein endothelial cells(HUVECs) with Kadsura. coccinea fruits’ epicarp phenolic extracts at the concentrations of 50–200 µg/mL improved the cell viability after exposure to H2O2 significantly, and inhibited malonaldehyde(MDA) and reactive oxygen species(ROS) overproduction, as well as enhancing the content of superoxide dismutase (SOD) and glutathione Reductase (GR. This study proved that K. coccinea is a natural resource of phenolics rich with potential antioxidant ability, which may be valuable for developing nutraceuticals and dietary supplements.

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Section: Resultsmentioning

confidence: 99%

Phenolics Profile and Protective Effect on Injuried HUVEC Cells of Epicarp Extracts from Kadsura coccinea

Yang

Cheng

et al. 2022

Foods

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“…In addition, the effective chromatographic separation of analytes is important to evade ionization intrusion in the mass source, which will be beneficial for refining the sensitivity and accuracy in the entire analysis. 23 Therefore, some critical parameters such as column selection, mobile phases, and gradient programs can significantly influence the chromatographic separation ability. These optimized conditions will be helpful to improve the peak resolution, boost the signal response, and shorten the running time for the simultaneous analysis of 16 targeted phenolic components.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

A Multidimensional UHPLC-DAD-QTOF-IMS Gradient Approach for Qualitative and Quantitative Investigation of Citrus and Malus Fruit Phenolic Extracts and Edibles

Dadwal

Joshi

Gupta

2021

ACS Food Sci. Technol.

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A simple, rapid, and reproducible method for detecting 16 phenolics including marker flavonoids, polymethoxyflavones (PMFs), dihydroxychalcones, and phenolic acids was developed using ultrahigh-performance liquid chromatography-diode array detector-quadrupole time-of-flight-ion mobility mass spectrometry (UHPLC-DAD-QTOF-IMS). The effectiveness of the method was assessed through limit of detection (0.0009–0.16 μg/mL), limit of quantification (0.0027–0.45 μg/mL), and standard calibrations to validate at an optimized flow rate of 0.2 mL/min. Hesperidin as a major flavonoid and tangeretin as a PMF were quantified in Citrus extracts, whereas phloridzin, a major dihydroxychalcone, was quantified in Malus fruit. Freeze-dried and market edibles were also analyzed, compared, and quantified. The two-dimensional IMS mode confirmed the presence of naringin, hesperidin, nobiletin, and tangeretin in Citrus at frame numbers 666, 706, 1260, and 1301, respectively, in the positive mode. Similarly, Malus samples showed phloretin and phloridzin confirmed at 890 and 1198 frames, respectively, in the negative mode. The present analytical approach aids the higher applicability in quality assurance and development of food and phenolic extracts derived from Citrus and Malus fruits in a single shot.

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“…Efficient chromatographic separation of analytes is important to avoid ionization interference in the MS source, which is good for improving the sensitivity and accuracy of the entire analysis [30] . Initially, the Waters ACQUITY UPLC ® BEH C18 column (100 mm × 2.1 mm, 1.7 μm) exhibited excellent performance by comparing several UPLC-analysis columns in our laboratory.…”

Section: Resultsmentioning

confidence: 99%

“…The validation of the proposed quantitation method was performed according to international guidelines in terms of specificity, linearity, sensitivity, stability, repeatability, accuracy and matrix effect [30] .…”

Section: Resultsmentioning

confidence: 99%

Research for improvement on the extract efficiency of lignans in traditional Chinese medicines by hybrid ionic liquids: As a case of Suhuang antitussive capsule

et al. 2021

Ultrasonics Sonochemistry

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Simultaneous quantification of eleven bioactive phenolic compounds in pigeon pea natural resources and in vitro cultures by ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS)

Cited by 21 publications

References 37 publications

Phenolics Profile and Protective Effect on Injuried HUVEC Cells of Epicarp Extracts from Kadsura coccinea

Phenolics Profile and Protective Effect on Injuried HUVEC Cells of Epicarp Extracts from Kadsura coccinea

A Multidimensional UHPLC-DAD-QTOF-IMS Gradient Approach for Qualitative and Quantitative Investigation of Citrus and Malus Fruit Phenolic Extracts and Edibles

Research for improvement on the extract efficiency of lignans in traditional Chinese medicines by hybrid ionic liquids: As a case of Suhuang antitussive capsule

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