Simultaneous quantification of eight bioactive secondary metabolites from Codonopsis ovata by validated high performance thin layer chromatography and their antioxidant profile

J of Separation Science

Jan

Wani

et al. 2018

Self Cite

Chemical investigation was carried out to examine the risk assessment, dissipation behavior, persistence, and half-life period of quinalphos in/on green pea fruit by spraying quinalphos at fruiting stage followed by another application after 10-day interval. The samples were extracted by using the quick, easy, cheap, effective, rugged, and safe method, and the residues of quinalphos were analyzed by gas chromatography with electron capture detector. Herein, we report a novel, accurate, and cost-effective gas chromatography method for the determination of average deposits of quinalphos in/on green pea. The initial deposits and half-life of quinalphos were found to be 1.20 mg/kg and 2.77 days, respectively, following the application of insecticide. Residues of quinalphos reached below detection limit of 0.05 mg/kg after 10 days at recommended dosage. For risk assessment studies, the tenth day will be safe for consumers for consumption of green pea. The developed method is simple, selective, and repeatable, and it can be extended for quinalphos-based standardization of herbal formulations containing green pea and its use in pesticide industries.

Section: Resultsmentioning

confidence: 96%

Risk assessment, dissipation behavior and persistence of quinalphos in/on green pea by gas chromatography with electron capture detector

J of Separation Science

Jan

Wani

et al. 2018

Self Cite

“…The known amount of investigated compound was added to the accurately weighed portion of plant extract, processed and analysed. The recovery percentage was calculated by the equation .

\begin{matrix} left Recovery (() %) & left = & left ((), amount found - amount present) / \\ left amount spiked \times 100 \end{matrix}

…”

Section: Validation Of the Methodsmentioning

confidence: 99%

“…Exhaustive literature survey revealed that various methods (UV spectrophotometry, GC, GC‐MS, HPTLC, LC‐MS/MS) can be employed for the fingerprint profiles and quantification of amino acids from natural sources , but such methods are either tedious or lack sensitivity. However, RP‐HPLC appears the instrumental approach of choice affording fingerprint profiles and quantitative analysis of desired components.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of 14 amino acids from five plants by validated reversed‐phase high‐performance liquid chromatography

Fayaz

Separation Science Plus

Bhat

et al. 2018

Self Cite

Madhuca longifolia, Cassia tora, Cocculus hirsutus, Celosea argentea and Alternanthera sessilis plants were scrutinized to determine the amino acid content by a validated method. Herein we report a novel, accurate and cost‐effective reversed‐phase high‐performance liquid chromatography method for the simultaneous determination and quantification of 14 amino acids. The developed method was validated as per the International Conference on Harmonisation guidelines. All calibration curves showed a good linear relationship (r > 0.9928) within test range. Precision was assessed by intra‐ and inter‐day tests with relative standard deviations less than 2%. Recovery of 98.38–99.60% with relative standard deviations <1.00% was observed. On quantification, glutamic acid, aspartic acid, alanine, methionine, glycine, leucine, valine and threonine were present in major quantity from different parts of selected plants which is advantageous as they control most of the physiological activities of the body. The proposed method can be used for the simultaneous estimation of 14 amino acids in one run and can be extended for amino acid‐based standardization of herbal formulations containing these plants and its use in pharmaceutical industries.

“…Therefore, RP‐HPLC appears the helpful approach of choice affording identification, separation and quantification of pesticides . In present communication with continuation of our previous studies on application of chromatography, risk assessment, persistence and simultaneous quantification of carbendazim and triazophos has been evaluated by validated reversed phase high performance liquid chromatography.…”

Section: Introductionmentioning

confidence: 96%

Dissipation, risk assessment, half‐life period and method validation of carbendazim and triazophos in green pea by high‐performance liquid chromatography

Wani

Separation Science Plus

Jan

et al. 2019

Self Cite

Herein, the simultaneous quantification, dissipation, risk assessment, half‐life period and method validation of carbendazim and triazophos by high‐performance liquid chromatography has been described. The developed method was validated as per the sante guidelines 2017. All calibration curves showed a good linear relationship (r > 0.99) within test range. Precision was assessed by intra‐ and inter‐day tests with relative standard deviations less than 5%. Recovery was observed within acceptable limit. The residue level of carbendazim and triazophos residues were observed upto the third day and reached below the detection limit on green pea on the fifth day after treatment with carbendazim and triazophos formulation at recommended dosages. Based on the findings, waiting period of 5 days is recommended for consumption of green pea. The presented study would be helpful for safe usage of carbendazim and triazophos on green pea and to prevent any health risk to consumers. The developed method is simple, easy, sensitive and repeatable with a short run time, and can be extended for residues based standardisation of pesticide formulations containing green pea and its use in pesticide industries.