Simultaneous quantification of 14 amino acids from five plants by validated reversed‐phase high‐performance liquid chromatography

Abstract

Madhuca longifolia, Cassia tora, Cocculus hirsutus, Celosea argentea and Alternanthera sessilis plants were scrutinized to determine the amino acid content by a validated method. Herein we report a novel, accurate and cost‐effective reversed‐phase high‐performance liquid chromatography method for the simultaneous determination and quantification of 14 amino acids. The developed method was validated as per the International Conference on Harmonisation guidelines. All calibration curves showed a good linear relationship (r > 0.9928) within test range. Precision was assessed by intra‐ and inter‐day tests with relative standard deviations less than 2%. Recovery of 98.38–99.60% with relative standard deviations <1.00% was observed. On quantification, glutamic acid, aspartic acid, alanine, methionine, glycine, leucine, valine and threonine were present in major quantity from different parts of selected plants which is advantageous as they control most of the physiological activities of the body. The proposed method can be used for the simultaneous estimation of 14 amino acids in one run and can be extended for amino acid‐based standardization of herbal formulations containing these plants and its use in pharmaceutical industries.

Dissipation and gas chromatographic method for the determination of profenofos residues in/on green pea and cucumber

“…Gas chromatography (GC) appears to be a suitable choice for the identification, separation and quantification of profenofos. In continuation of our previous studies (Ahmad Dar et al, 2020; Dangroo et al, 2016; Dangroo et al, 2014; Dar et al, 2014; Dar et al, 2015; Dar et al, 2016; Dar et al, 2018; Dar et al, 2019; Fayaz et al, 2018; Jan et al, 2018; Khan et al, 2013; Khan et al, 2012; Singh et al, 2018; Wani et al, 2019a) on the application of chromatography for fingerprinting and chromatographic method development to assure quality control of vegetables/fruits/medicinal plants, herein we report a validated analytical method for the quantification of profenofos from green pea and cucumber, as well as risk assessment and evaluation of dissipation behaviour, persistence and half‐life.…”

Dissipation, risk assessment, half‐life period and method validation of carbendazim and triazophos in green pea by high‐performance liquid chromatography

“…Therefore, RP‐HPLC appears the helpful approach of choice affording identification, separation and quantification of pesticides . In present communication with continuation of our previous studies on application of chromatography, risk assessment, persistence and simultaneous quantification of carbendazim and triazophos has been evaluated by validated reversed phase high performance liquid chromatography.…”

Method validation and simultaneous quantification of eight organochlorines/organophosphates in apple by gas chromatography

“…Therefore, gas chromatography (GC) appears the appropriate choice affording identification, separation and quantification of pesticides. In continuation of our previous studies [19][20][21][22][23][24][25][26][27][28] on application of chromatography for fingerprinting and chromatographic method development to assure quality control of vegetables/fruits/medicinal plants, a validated analytical method has been developed for the simultaneous quantification of eight pesticides ( Fig. 1) from apple: chlorpyrifos-methyl (1), chlorpyrifos (2), quinolphos (3), profenofos (4), myclobutnil (5), ethion (6), fenpropathrin (7) and cypermethrin (8).…”