Simultaneous quantification of 5-FU, 5-FUrd, 5-FdUrd, 5-FdUMP, dUMP and TMP in cultured cell models by LC-MS/MS

Carli, Delphine; Honorat, Mylène; Cohen, Sabine; Megherbi, Mehdi; Vignal, B; Dumontet, Charles; Payen, Léa; Guitton, Jérôme

doi:10.1016/j.jchromb.2009.07.004

Cited by 29 publications

(35 citation statements)

References 20 publications

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“…In a subset of samples, the MRP inhibitor MK571 was added during the incubation phase at a concentration of 10 M. Supernatants and cell lysates were stored at Ϫ20°C until determination. Assessment of 5-FdUMP was performed by liquid chromatography-mass spectrometry as described previously (Honorat et al, 2008;Carli et al, 2009 …”

Section: Methodsmentioning

confidence: 99%

Drug Efflux Transporter Multidrug Resistance-Associated Protein 5 Affects Sensitivity of Pancreatic Cancer Cell Lines to the Nucleoside Anticancer Drug 5-Fluorouracil

Nambaru

Hübner²,

Köck³

et al. 2010

Drug Metab Dispos

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ABSTRACT:Pancreatic adenocarcinoma is one of the malignancies that is highly resistant to therapy and among the leading causes of cancer-related death. Several factors may influence pancreatic cancer resistance, and expression of ATP-binding cassette transport proteins is one of the major mechanisms of drug resistance. Members of this family's C-branch, also referred to as multidrug resistanceassociated proteins (MRPs), might be of particular interest be-

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Section: Methodsmentioning

confidence: 99%

Drug Efflux Transporter Multidrug Resistance-Associated Protein 5 Affects Sensitivity of Pancreatic Cancer Cell Lines to the Nucleoside Anticancer Drug 5-Fluorouracil

Nambaru

Hübner²,

Köck³

et al. 2010

Drug Metab Dispos

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“…For BM, accuracy and precision were respectively assessed by the mean relative percentage deviation from the nominal concentration and the within-run precision (WRP) and between-run precision (BRP). These parameters were calculated with the method described by Carli et al [9] using Statview software for Windows, version 5.0 (SAS Institute, Cary, NC, USA). The within-run precision of the assay (each of the two concentration levels analyzed 5 times) was 4.99% at low and 1.59% at high concentration level.…”

Section: Methods Validationmentioning

confidence: 99%

Postmortem measurement of caffeine in bone marrow: Influence of sample location and correlation with blood concentration

Cartiser¹,

Bévalot²,

Chatenay³

et al. 2011

Forensic Science International

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“…The proposed method was rugged, precise, accurate, and well suited to support [45]. 5-FU have also been quantified in cultured cell model by LC-MS coupled to MS detection technique over a concentration range of 2.5-150 ng mL -1 , without any matrix effect [46]. A reliable, simple, isocratic stability indicating HPLC-UV method have been developed for 5-FU analysis using thymine as an internal standard within the concentration range of 0.1-2.0 lg mL -1 [47].…”

Section: Hybrid Instrumental Techniques For 5-fu Analysis In Biologicmentioning

confidence: 99%

A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

2015

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The most important objectives that are frequently found in bio-analytical chemistry involve applying tools to relevant medical/biological problems and refining these applications. Developing a reliable sample preparation step, for the medical and biological fields is another primary objective in analytical chemistry, in order to extract and isolate the analytes of interest from complex biological matrices. Since, main inborn errors of metabolism (IEM) diagnosable through uracil analysis and the therapeutic monitoring of toxic 5-fluoruracil (an important anticancerous drug) in dihydropyrimidine dehydrogenase deficient patients, require an ultra-sensitive, reproducible, selective, and accurate analytical techniques for their measurements. Therefore, keeping in view, the diagnostic value of uracil and 5-fluoruracil measurements, this article refines several analytical techniques involved in selective recognition and quantification of uracil and 5-fluoruracil from biological and pharmaceutical samples. The prospective study revealed that implementation of molecularly imprinted polymer as a solid-phase material for sample preparation and preconcentration of uracil and 5-fluoruracil had proven to be effective as it could obviates problems related to tedious separation techniques, owing to protein binding and drastic interferences, from the complex matrices in real samples such as blood plasma, serum samples.

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Simultaneous quantification of 5-FU, 5-FUrd, 5-FdUrd, 5-FdUMP, dUMP and TMP in cultured cell models by LC-MS/MS

Cited by 29 publications

References 20 publications

Drug Efflux Transporter Multidrug Resistance-Associated Protein 5 Affects Sensitivity of Pancreatic Cancer Cell Lines to the Nucleoside Anticancer Drug 5-Fluorouracil

Drug Efflux Transporter Multidrug Resistance-Associated Protein 5 Affects Sensitivity of Pancreatic Cancer Cell Lines to the Nucleoside Anticancer Drug 5-Fluorouracil

Postmortem measurement of caffeine in bone marrow: Influence of sample location and correlation with blood concentration

A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

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