Simultaneous quantification of 14 bioactive constituents in <i>Forsythia Suspensa</i> by liquid chromatography–electrospray ionisation–mass spectrometry

Yang, Chao; Wang, Qiao; Shi, Xiaowei; Zhang, Xiaowei; Sheng, Xiaona; Zhang, Lantong

doi:10.1002/pca.1194

Cited by 46 publications

(32 citation statements)

References 4 publications

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“…Several papers have reported the isolation, purification, and analysis of phillyrin [10][11][12][13][14][15][16][17]. Ultrasonic-assisted extraction (UAE), heat reflux extraction (HRE), decoction extraction (DE), and microwave-assisted extraction (MAE) had been applied to extract the active compounds from F. suspense [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Ultrasonic-assisted extraction (UAE), heat reflux extraction (HRE), decoction extraction (DE), and microwave-assisted extraction (MAE) had been applied to extract the active compounds from F. suspense [10][11][12]. However, methods based on liquid phase separation techniques (LC and LC-MS) had often been used to analyse the phillyrin in F. suspense and its related preparations [13][14][15][16][17][18][19]. Electron ionization-mass spectrometry (EI-MS) has been used to analyze different kinds of compounds, but it is more suitable for a single analyte [20].…”

Section: Introductionmentioning

confidence: 99%

“…LC-MS has also become a powerful tool in the online structural characterization of various natural compounds owing to its high sensitivity, great separation efficiency and unique structure analysis ability [21]. For example, Cui et al had establish an LC-MS method for simultaneous quantification of 14 bioactive constituents of F. suspense in different places of China and different parts of this herb in negative mode [16]. Wang et al used time-of-flight mass spectrometry (TOF-MS) to confirm the substituents of phenylpropanoids in the fruits of F. suspense in negative mode [18].…”

Section: Introductionmentioning

confidence: 99%

“…To date, determination of phillyrin by LC-ultraviolet (UV) [14,15] and LC-MS [16] was reported. However, there is no comprehensive electrospray ionization (ESI)-MS fragmentation study of phillyrin.…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive characterization and quantification of phillyrin in the fruits ofForsythia suspensaand its medicinal preparations by liquid chromatography-ion trap mass spectrometry

Chen

et al. 2016

Acta Chromatographica

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Summary.A rapid and sensitive method for the identification and quantification of phillyrin (POG) in Forsythia suspense is described. The phillyrin standard solution was directly infused into the ion trap mass spectrometers (IT-MS) for collecting the MS n spectra. The electrospray ionization (ESI) mass spectral fragmentation pathway of phillyrin was proposed, and the ESI-MS n fragmentation behavior of phillyrin was deduced in detail. The major product ion at m/z 355 belongs to furofuran, which was formed by loss the glucopyranoside (180 Da), and the characteristic fragment ions m/z 473, 395, 337, 309, and 249 were observed. The loss of 18 Da could arise from two different fragmentation pathways, and the observed ion was composed of a mixture of two different structural ions. Quantification of phillyrin was assigned in positive-ion mode at a product ion at m/z 557 → 355 by liquid chromatography-mass spectrometry (LC-MS). The LC-MS method was validated for linearity, sensitivity, accuracy, and precision and then used to determine the content of the phillyrin. Lastly, the LC-MS method was successfully applied to determine phillyrin in real sample F. suspense and three of its medicinal preparations in the positive mode at the first time.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comprehensive characterization and quantification of phillyrin in the fruits ofForsythia suspensaand its medicinal preparations by liquid chromatography-ion trap mass spectrometry

Chen

et al. 2016

Acta Chromatographica

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“…All marker compounds showed good linearity (r 2 > 0.9994) in the linear range. LOD and the LOQ were determined for each marker compound at the signal-to-noise ratio (S/N) of 3 and 10, respectively (Cui et al, 2010). The value of LOD and LOQ were in the range of 0.01-0.13 µg/mL and 0.03-0.41 µg/mL, respectively.…”

Section: Methods Validationmentioning

confidence: 99%

Simultaneous determination of eight marker compounds in the traditional herbal medicine, sipjundaebo-tang by HPLC-DAD

et al. 2011

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Sipjundaebo-tang, known as a traditional herbal medicine, has been used in the treatment of anemia, inflammation, and tumor. For simultaneous determination of eight components, namely 5-HMF, paeoniflorin, ferulic acid, cinnamaldehyde, decursinol, 6-gingerol, decursin, and glycyrrhizin in Sipjundaebo-tang, a high performance liquid chromatography-diode array detector (HPLC-DAD) method was established. In order to develop and validate this HPLC-DAD method, C(18) column (S-5 μm, 4.6 × 250 mm) was used with gradient mobile phase at the column temperature of 35°C. The mobile phase was consisted of water and methanol containing 0.1% trifluoroacetic acid. UV wavelength was set at 230, 254, 280, and 300 nm. Validation of the analytical method was evaluated by linearity, precision, and accuracy test. Calibration curve for eight marker compounds showed good linearity with R(2) > 0.9994. Limits of detection and Limits of quantification ranged from 0.01 μg/mL to 0.13 μg/mL and 0.03 μg/mL to 0.41 μg/mL, respectively. The relative standard deviations (RSDs) value of precision test, intra-day and inter-day test, was less than 1.14% and 2.54%, respectively. The results of accuracy test were varied from 98.31% to 104.88% with RSD < 2%. This developed simultaneous determination method was efficient to the quality control of Sipjundaebo-tang.

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Phytochemical Profiling by UPLC‐ESI‐QTOF‐MS of Kalaharia uncinata (Schinz) Moldenke, Widely Used in Traditional Medicine in DR Congo

Kibungu Kembelo,

Tuenter,

Vanhove

et al. 2023

Chemistry & Biodiversity

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Kalaharia uncinata (Schinz) Moldenke (Lamiaceae), is a tropical erect bushy shrub or subshrub, endemic of Southern Africa, and widely used in the pharmacopoeia against upper respiratory tract infections. A previously conducted ethnobotanical survey revealed that it is believed to contain bioactive substances. However, no relevant phytochemical information was available. This study aimed to perform a phytochemical characterization of K. uncinata and to discuss the potential bioactivity of the identified phytochemical constituents based on documented data. Ultra‐performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight mass spectrometry (UPLC‐ESI‐QTOF‐MS) was used for profiling and identification of the main phytochemical constituents from leaf extracts (MeOH 90%, dichloromethane, EtOAc, n‐BuOH, n‐hexane and residue) of K.uncinata. Twenty‐four constituents, representing mainly flavonoids (14), followed by phenylethanoid glycosides (7), phenolic acids (2), and an iridoid glycoside (1) were tentatively identified. K. uncinata contains interesting compounds. Most of the identified compounds are documented to have antiviral and anti‐inflammatory properties, which could possibly be the rationale behind the use of K. uncinata against upper respiratory tract infection. Further studies would be required to elucidate the exact structures of the tentatively identified compounds, as well as to evaluate their efficacy against upper respiratory tract infections and to evaluate their effectiveness.

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Simultaneous quantification of 14 bioactive constituents in Forsythia Suspensa by liquid chromatography–electrospray ionisation–mass spectrometry

Abstract: The proposed method was successfully applied for the quantitative analysis of 14 constituents in 12 Forsythia suspensa samples.

Cited by 46 publications

References 4 publications

Comprehensive characterization and quantification of phillyrin in the fruits ofForsythia suspensaand its medicinal preparations by liquid chromatography-ion trap mass spectrometry

Comprehensive characterization and quantification of phillyrin in the fruits ofForsythia suspensaand its medicinal preparations by liquid chromatography-ion trap mass spectrometry

Simultaneous determination of eight marker compounds in the traditional herbal medicine, sipjundaebo-tang by HPLC-DAD

Phytochemical Profiling by UPLC‐ESI‐QTOF‐MS of Kalaharia uncinata (Schinz) Moldenke, Widely Used in Traditional Medicine in DR Congo

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