Simultaneous determination of eight marker compounds in the traditional herbal medicine, sipjundaebo-tang by HPLC-DAD

Abstract

Sipjundaebo-tang, known as a traditional herbal medicine, has been used in the treatment of anemia, inflammation, and tumor. For simultaneous determination of eight components, namely 5-HMF, paeoniflorin, ferulic acid, cinnamaldehyde, decursinol, 6-gingerol, decursin, and glycyrrhizin in Sipjundaebo-tang, a high performance liquid chromatography-diode array detector (HPLC-DAD) method was established. In order to develop and validate this HPLC-DAD method, C(18) column (S-5 μm, 4.6 × 250 mm) was used with gradient mobile phase at the column temperature of 35°C. The mobile phase was consisted of water and methanol containing 0.1% trifluoroacetic acid. UV wavelength was set at 230, 254, 280, and 300 nm. Validation of the analytical method was evaluated by linearity, precision, and accuracy test. Calibration curve for eight marker compounds showed good linearity with R(2) > 0.9994. Limits of detection and Limits of quantification ranged from 0.01 μg/mL to 0.13 μg/mL and 0.03 μg/mL to 0.41 μg/mL, respectively. The relative standard deviations (RSDs) value of precision test, intra-day and inter-day test, was less than 1.14% and 2.54%, respectively. The results of accuracy test were varied from 98.31% to 104.88% with RSD < 2%. This developed simultaneous determination method was efficient to the quality control of Sipjundaebo-tang.

Co-Administration of Gagam-Sipjeondaebo-Tang and Ibuprofen Alleviates the Inflammatory Response in MPTP-Induced Parkinson’s Disease Mouse Model and RAW264.7 Macrophages

“…For instance, curcumin has been reported as pan-assay-interference compounds and invalid metabolic panacea, thus raising concerns about its medicinal properties [42][43][44]. Several compounds of GST have been found, yet, the effect of each chemical compound still needs further investigation [13,[45][46][47][48]. It is necessary to continue further studies in order to provide reliable quality standards of medicinal herbs.…”

Natural Korean Medicine Dang-Gui: Biosynthesis, Effective Extraction and Formulations of Major Active Pyranocoumarins, Their Molecular Action Mechanism in Cancer, and Other Biological Activities

“…The major roles of AGN’s active chemical compounds in several biological activities are depicted in Figure 2 . In addition, decursin and/or DA were reported in some herbal formulations such as ka-mi-kae-kyuk-tang (KMKKT) [ 36 ], bangpungtongsung-san [ 37 ], sanghwa tang (SHT) [ 38 ], LMK02-jangwonhwan [ 39 ], sipjundaebo-tang [ 40 ] and ojeok-san (OJS) [ 37 ].…”

Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)

“…Glycyrrhizic acid is the major constituent of licorice root capsule (LRC) as a premium herbal dietary supplement, and is widely used 213 against digestion problem, as relieving stomach cramps and immune booster in China (Yang et al 2012). Different analytical methods have been reported for measurement of glycyrrhizic acid in plant extracts including high performance liquid chromatography (HPLC) (Yang et al 2011;Seo et al 2012), high performance thin layer chromatography (HPTLC) (Gantait et al 2010), gas chromatography mass spectrometry (GC-MS) (Guillaume 1999;Lisec et al 2006), thin-layer chromatographic (TLC) (Yang et al 1991;Faisal et al 2009), and capillary electrophoresis (Chen and Sheu 1993). Amount of glycyrrhizic acid in the plant is highly variable (Xie et al 2010;Alam et al 2014;Zhou et al 2015).…”