Simultaneous qualitation and quantitation of natural trans-1,4-polyisoprene from Eucommia ulmoides Oliver by gel permeation chromatography (GPC)

Guo, Tao; Liu, Yuanbo; Yan-Xiu, Wei; Ma, Xiang; Fan, Qiu-Ming; Ni, Jing; Zhang, Yin; Liu, Jiahui; Wang, Sai; Dong, Yiyang; Zhang, Jiaheng; Zhang, Liqun; Su, Haijia; Tan, Tianwei

doi:10.1016/j.jchromb.2015.09.007

Cited by 26 publications

(11 citation statements)

References 21 publications

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“…The use of Eu-rubber with high Mw is equivalent to that of NR and can be used as a "green tire" and the Eu-rubber with low Mw is characterized by low melting point and strong hardness, which can be used as medically functional materials, shape memory materials and additives (De Barros et al 1992;Kovuttikulrangsie and Sakdapipanich, 2005). Additionally, Those Eu-rubber with the same Mw and a narrower MWD have greater strength and plasticity, while other gutta-percha with wider MWD is better elastic, more wear-resistant and heat-resistant (Westall 1968;Guo et al 2015). GPC analysis of MWD of Eu-rubber molecules in E. ulmoides leaf collected in October showed that there were five characteristic peaks, including high molecular weight (HMw), medium molecular weight (MMw) and low molecular weight (LMw), while MWD of Eu-rubber molecules in E. ulmoides samara collected at the same month contained two or three characteristic peaks.…”

Section: Discussionmentioning

confidence: 99%

Fatty Acid Composition of Eucommia Rubber Particles, Content and Molecular Weight Distribution of Eu-rubber in Eucommia Ulmoides Leaves and Samaras

Qin

Yuan

Yang

et al. 2020

Preprint

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[Backgroud] Eucommia ulmoides rubber particles (EuRPs) wrapped by a monolayer protein-lipid membrane are speculated to an important site for the biosynthesis of E. ulmoides rubber (Eu-rubber), and the types and contents of these protein and lipid varied in different E. ulmoides organs which might indicate the difference of Eu-rubber biosynthesis in EuRPs from different organs and tissues. In this paper, the morphological structure and fatty acid composition of EuRPs in leaves and samaras collected in October, 2019 were compared, and molecular weight (Mw) and molecular weight distribution (MWD) were also analyed by GPC system. [Results] The results showed that small rubber particles (SRPs, 1~4 μm) and medium rubber particles (MRPs, 4~7 μm) from leaves and samaras respectively accounted for 39%, 53% and 38%, 34%, while the amount of the large rubber particles (LRPs, 7~11 μm) were 23% and 13%, which indicated there was no significance in leaf and samara SRPs while the amount of MRPs and LRPs in leaf both were more than that of in samara. Synchronously, the determination results of fatty acids (FAs) compositon showed three of the 35 kinds FAs were uniquely detected in leaf rubber particles (LeRPs), which included eleven carbonic (C11:0), thirteen carbonic (C13:0) and arachidic acids (C20:0), while γ-linolenic acid (C18:3n6) was exclusively found in samara rubber particles (SaRPs) with a lower content. Additioanlly, five characteristic distribution peaks (CDPs) of Eu-rubber from leaves were detected while there were only 2-3 CDPs in samaras; Further, the MWD analysis suggested that the MWD of Eu-rubber in E. ulmoides samaras and leaves were 40.61 and 6.76 respectively, which indicated the more Eu-rubber molecules with various Mw existed in samara compared with those in leaf. [Conclusions] Together, we concluded that average size of LeRPs was bigger than SaRPs in October, and 3 kinds of saturated fatty acid (SAF) were unique to the leaves while only one of unsaturated fatty acid (UFA) was sole to SaRPs. Simultaneously, the Eu-rubber in samara in October possessed more rubber molecules with various molecular weights compared with the contemporaneous leaves.

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Section: Discussionmentioning

confidence: 99%

Fatty Acid Composition of Eucommia Rubber Particles, Content and Molecular Weight Distribution of Eu-rubber in Eucommia Ulmoides Leaves and Samaras

Qin

Yuan

Yang

et al. 2020

Preprint

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“…Hence, the bark and samara only contain high-molecular-weight gutta-percha, while leaves contain low-and high-molecular-weight gutta-percha, which is consistent with the literature. 54 Fourier Transform Infrared Spectroscopy of Gutta-Percha. FTIR spectra were collected for gutta-percha from the leaves, samara, and bark.…”

Section: Characterization Of Molecular Weight Distribution (Mwd)mentioning

confidence: 99%

Potential Use of Limonene as an Alternative Solvent for Extraction of Gutta-Percha from Eucommia ulmoides

Cui

Yang

Liu

et al. 2022

ACS Sustainable Chem. Eng.

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Limonene was used as an extraction solvent to obtain gutta-percha from leaves, samara, and bark of Eucommia ulmoides. The extraction conditions for the gutta-percha of E. ulmoides were optimized using a set of optimization processes. The optimized conditions were a liquid−solid ratio (L/S ratio) of 19.7 mL/g, extraction temperature of 134 °C, and extraction time of 1.8 h for the bark; an extraction temperature of 128 °C, extraction time of 1.7 h, and L/S ratio of 14 mL/g for the samara; and an extraction time of 1.4 h, L/S ratio of 15 mL/g, and reaction temperature of 138 °C for the leaves. Under these optimized extraction conditions, the actual extraction yields of gutta-percha were 55.32 ± 2.53 mg/g (bark), 81.14 ± 3.84 mg/g (samara), and 22.15 ± 1.08 mg/g (leaves). The gutta-percha obtained by limonene extraction was evaluated by thermogravimetric and differential thermogravimetric tests, gel permeation chromatography, differential scanning calorimetry, and Fourier transform infrared spectroscopy. Limonene has promising potential to replace traditional petroleum-based solvents to isolate gutta-percha from E. ulmoides.

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“…The mobile phase is toluene or tetrahydrofuran solution at a flow rate of 1.0 mL/min. Guo et al 35 proposed a method that enables simultaneous analysis of the content and molecular-weight distribution of EUR by gel permeation chromatography/size-exclusion chromatography (GPC-SEC). The results showed that the contents of EUR from pericarp, bark, and leaves are 13.8%, 8.5%, and 3.5%, respectively.…”

Section: Properties Ofmentioning

confidence: 99%

“…It is reported that the molecular weight distribution of EUR in E. ulmoides pericarp, bark, and leaves ranges from 4 × 10 4 to 2 × 10 6 , 3 × 10 4 to 2 × 10 6 , and 1 × 10 3 to 6 × 10 4 Da, respectively. 35 The molecular-weight distribution demonstrated a unimodal distribution from bark, a bimodal distribution from leaves, and a unimodal distribution with a tiny peak shoulder from pericarp. 71,139 In addition, the molecular weight and PDI of EUR are dependent on their origin and extraction process, and the molecular weight is affected by the annual growth cycle and clones of E. ulmoides.…”

Section: Properties Ofmentioning

confidence: 99%

Natural Polymer Eucommia Ulmoides Rubber: A Novel Material

Wei

Peng

et al. 2021

J. Agric. Food Chem.

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As the second natural rubber resource, Eucommia ulmoides rubber (EUR) from Eucommia ulmoides Oliver is mainly composed of trans-1,4-polyisoprene, which is the isomer of natural rubber cis-1,4-polyisoprene from Hevea brasiliensis. In the past few years, the great potential application of EUR has received increasing attention, and there is a growing awareness that the natural polymer EUR could become an emerging research topic in field of the novel materials due to its unique and excellent duality of both rubber and plastic. To gain insight into its further development, in this review, the extraction, structure, physicochemical properties, and modification of EUR are discussed in detail. More emphasis on the potential applications in the fields of the environment, agriculture, engineering, and biomedical engineering is summarized. Finally, some insights into the challenges and perspectives of EUR are also suggested.

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Simultaneous qualitation and quantitation of natural trans-1,4-polyisoprene from Eucommia ulmoides Oliver by gel permeation chromatography (GPC)

Cited by 26 publications

References 21 publications

Fatty Acid Composition of Eucommia Rubber Particles, Content and Molecular Weight Distribution of Eu-rubber in Eucommia Ulmoides Leaves and Samaras

Fatty Acid Composition of Eucommia Rubber Particles, Content and Molecular Weight Distribution of Eu-rubber in Eucommia Ulmoides Leaves and Samaras

Potential Use of Limonene as an Alternative Solvent for Extraction of Gutta-Percha from Eucommia ulmoides

Natural Polymer Eucommia Ulmoides Rubber: A Novel Material

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