Simultaneous Production of Lipases and Biosurfactants in Solid-State Fermentation and Use in Bioremediation

Kreling, Naiara Elisa; Simon, Viviane; Fagundes, Victória Dutra; Thomé, Antônio; Colla, Luciane Maria

doi:10.1061/(asce)ee.1943-7870.0001785

Cited by 21 publications

(4 citation statements)

References 40 publications

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“…The fungi belonging to the gender Aspergillus and Rhyzopus used in SSF for the obtainment of the extracts in the study have been previously reported due to their antimicrobial potential (Chamkhi et al 2018 ; Geetanjali et al 2018 ; Zhang et al 2016 ). During the fermentative process, fungi are able to produce compounds with antimicrobial activity which results in the higher antimicrobial potential observed for the extracts in the study, compared to non-fermented ones (Dong et al 2016 ; Kreling et al 2020 ; Lourenço et al 2018 ; Mohamed et al 2016 ). Feitosa et al ( 2020 ) reported the antimicrobial activity of fermented extracts against B. subtilis after SSF of moringa leaves flour with the fungus A. niger while the unfermented extracts did not exhibit the same bioactivity.…”

Section: Resultsmentioning

confidence: 94%

Evaluation of biotechnological processing through solid-state fermentation of oilseed cakes on extracts bioactive potential

et al. 2023

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Oilseed cakes (OC) are natural sources of lignocellulosic biomass, produced every year in large amounts. In addition to their main applications as animal feed, plant or soil fertilizer, and compost, they present enormous potential for being used in biotechnological processes for the obtainment and extraction of valuable bioactive compounds. This work evaluated the effect of solid-state fermentation on the bioactive properties of extracts obtained from the bioprocessing of OC and evaluated the effect of solvents on the recovery of compounds with higher bioactive potential. A general decrease of EC50 values was observed for fermented extracts obtained using a mixture of water/methanol (1:1) as extraction solvent. A decrease in the minimum inhibitory concentration was observed for fermented water extracts compared to non-fermented. Additionally, growth inhibition of Listeria monocytogenes was observed when using aqueous methanolic fermented extracts. These extracts also exhibited a higher percentage of growth reduction against phytopathogenic fungi, and some extracts exhibited increased protection against genotoxic agents such as camptothecin and bisphenol A. It was demonstrated that bioprocessing of OC through SSF is an effective approach to obtaining valuable compounds with bioactive properties for use in the food, pharmaceutical or cosmetic industries.

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Section: Resultsmentioning

confidence: 94%

Evaluation of biotechnological processing through solid-state fermentation of oilseed cakes on extracts bioactive potential

et al. 2023

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“…The authors indicate that clayey soils, such as the one used in the present study, retain a percentage of the contaminant in their matrix, but this percentage is lower when using compounds of organic origin, such as lipases, biosurfactants, and solid culture medium. Kreling et al (2020) indicate values of 44% to adsorption. The values found in our study are similar to those veri ed by the author due to the high percentage of organic compounds added to the soil (10% in both studies).…”

Section: In Situ Production Of Biosurfactants and Lipases In Soil And...mentioning

confidence: 99%

“…The substrates used in the preparation of the culture media were wheat bran (Wb), corn cob (CC), sugarcane molasses (SCM) in the form of sludge from a storage tank for the production process of animal feed, obtained from the company Alisul Alimentos SA (Carazinho/RS/Brasil). The substrates were characterized regarding the contents of proteins, lipids, xed mineral residue (ash), moisture, total carbohydrates, particle size, and concentration of glucose present in the molasses residue (Kreling et al 2020).…”

Section: Preparation Of Culture Media and Experimental Designsmentioning

confidence: 99%

Co-production of Lipases and Biosurfactants by Bacillus Methylotrophicus in Solid State Fermentation for Use in Bioremediation

Kreling

Fagundes

Simon

et al. 2022

Preprint

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Production of biosurfactants and lipases via solid state fermentation (SSF) processes is still little explored when considering the use of bacteria. The potential application of solid matrices without the need for precipitation and recovery processes, helps to enable bioremediation processes. The aim of this work was the co-production of biocompounds via SSF using Bacillus methylotrophicus and the use of the fermented substrate in bioremediation of a soil contaminated with 20% of biodiesel. The biocompounds were produced using wheat bran and corncob (80/20), 75% moisture, 1% inducer (glycerol), 2% nitrogen and 1% sugar cane molasses, during 8 days. The bioremediation was evaluated for 90 days, and the assay containing biocompounds + fermented substrate + bacteria in its active form showed the highest biodegradation (72.08 ± 0.36% in 60 days). Maximum production of biosurfactants and lipases in soil was verified in 30 days of bioremediation. The possibility of co-production of two biocompounds in the same culture medium is confirmed, representing a cost reduction and potential feasibility of the bioprocess when considering industrial production scale, in addition to its environmental application in the treatment of oily contaminants.

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“…In other cases, biosurfactant production is also associated with the excretion of hydrolytic enzymes such as lipases, proteases and amylases [ 224 , 225 ]. The fungus Pleurotus sajor-caju simultaneously produces biosurfactants (4.01 gL −1 ) and ligninolytic enzymes (laccase, manganese peroxidase and lignin peroxidase) in solid-state cultivation with sunflower seed husks as substrate [ 226 ].…”

Section: New Production Technologiesmentioning

confidence: 99%

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

Silva

Banat

Giachini

et al. 2021

Bioprocess Biosyst Eng

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Biosurfactants are in demand by the global market as natural commodities that can be added to commercial products or use in environmental applications. These biomolecules reduce the surface/interfacial tension between fluid phases and exhibit superior stability to chemical surfactants under different physico-chemical conditions. Biotechnological production of biosurfactants is still emerging. Fungi are promising producers of these molecules with unique chemical structures, such as sophorolipids, mannosylerythritol lipids, cellobiose lipids, xylolipids, polyol lipids and hydrophobins. In this review, we aimed to contextualize concepts related to fungal biosurfactant production and its application in industry and the environment. Concepts related to the thermodynamic and physico-chemical properties of biosurfactants are presented, which allows detailed analysis of their structural and application. Promising niches for isolating biosurfactant-producing fungi are presented, as well as screening methodologies are discussed. Finally, strategies related to process parameters and variables, simultaneous production, process optimization through statistical and genetic tools, downstream processing and some aspects of commercial products formulations are presented.

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Simultaneous Production of Lipases and Biosurfactants in Solid-State Fermentation and Use in Bioremediation

Cited by 21 publications

References 40 publications

Evaluation of biotechnological processing through solid-state fermentation of oilseed cakes on extracts bioactive potential

Evaluation of biotechnological processing through solid-state fermentation of oilseed cakes on extracts bioactive potential

Co-production of Lipases and Biosurfactants by Bacillus Methylotrophicus in Solid State Fermentation for Use in Bioremediation

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

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