Simultaneous Pretreatment and Biohydrogen Production from Wheat Straw by Newly Isolated Ligninolytic Bacillus Sp. Strains with Two-Stage Batch Fermentation System

Shah, Tawaf Ali; Ali, Shehbaz; Afzal, Asifa; Tabassum, Romana

doi:10.1007/s12155-018-9936-x

Cited by 20 publications

(6 citation statements)

References 60 publications

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“…Highest LiP were secreted by strain S2 (70.968 ± 0.175 U/L), followed by S11Y (63.570 ± 0.021 U/L), and S23 (60.880  0.042 U/L). Based on the observation, strain S23 showed an absence of Lac activity and generated [20] [21], but it was detected in our study. Thus, it can be seen that the enzyme activities studied are comparable with the previous studies, indicating that the studied bacterial strains present promising results towards the production of lignin-degrading enzymes.…”

Section: Production Of Ligninolytic Enzymes By Bacterial Strainscontrasting

confidence: 58%

Production of Ligninolytic Enzymes from Thermophilic Bacterial Strains Isolated from Palm Oil Wastes

Riyadi

Azman

Yusof

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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The conversion of lignocellulosic biomass into bioethanol or biochemical products requires a crucial pre-treatment process to break down the recalcitrant lignin structure. Biological depolymerization of lignin using microbial enzyme appeared to be a promising pre-treatment alternative as it offers environmentally friendly treatment with lower energy requirements. In this study, three (3) thermophilic bacterial strains (S2, S11Y, S23) with lignin-degrading potential were previously isolated from palm oil wastes and identified as Stenotrophomonas sp., Bacillus subtilis, and Aeribacillus sp., respectively. These isolates demonstrated the capability to grow in a medium containing AL as the sole carbon source. Most isolates also demonstrated enzymatic activities toward lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lac) enzymes within a short period of 72 h. The highest LiP was secreted by strain S2 (70.97 ± 0.059 U/L), followed by S11Y (63.570 ± 0.021 U/L) and S23 (60.880 ± 0.042 U/L). MnP was generated with low activities of approximately 1.313 ± 0.037 U/L by S11Y, 0.364 ± 0.004 U/L by S2, and 0.215 ± 0.021 U/L by S23. For the Lac enzyme, low activities were detected only in S2 and S11Y, with 2.075 ± 0.612 U/L and 1.463 ± 0.116 U/L, respectively. This preliminary study appeared to show that the isolated thermophilic bacteria have the potential to be applied for lignin degradation application.

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Section: Production Of Ligninolytic Enzymes By Bacterial Strainscontrasting

confidence: 58%

Production of Ligninolytic Enzymes from Thermophilic Bacterial Strains Isolated from Palm Oil Wastes

Riyadi

Azman

Yusof

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…When 'cellulolytic archaea' norank_c_Bathyarchaeia was enriched and used in a bioaugmentation experiment, the daily methane yield rate was significantly increased by 24.3% [29]. According to Shah et al, the methane yield of wheat straw treated with lignin-degrading bacillus was 261.4% higher than that of untreated wheat straw [30]. Pretreatment of lignocellulosic substrate with cattle rumen fluid could increase methane production by 36.18% [31].…”

Section: Biogas/methane Productionmentioning

confidence: 99%

Enhanced Biogas Production by Ligninolytic Strain Enterobacter hormaechei KA3 for Anaerobic Digestion of Corn Straw

Zhang

Zhao

et al. 2021

Energies

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Lignin-feeding insect gut is a natural ligninolytic microbial bank for the sustainable conversion of crop straw to biogas. However, limited studies have been done on highly efficient microbes. Here, an efficient ligninolytic strain Enterobacter hormaechei KA3 was isolated from the gut microbiomes of lignin-feeding Hypomeces squamosus Fabricius, and its effects on lignin degradation and anaerobic digestion were investigated. No research has been reported. Results showed that strain KA3 had better lignin-degrading ability for corn straw with a higher lignin-degrading rate (32.05%) and lignin peroxidase activity (585.2 U/L). Furthermore, the highest cumulative biogas yield (59.19 L/kg-VS) and methane yield (14.76 L/kg-VS) were obtained for KA3 inoculation, which increased by 20% and 31%, respectively, compared to CK. Higher removal rates of COD, TS, and vs. of 41.6%, 43.11%, and 66.59% were also found. Moreover, microbial community diversity increased as digestion time prolonged in TG, and bacteria were more diverse than archaea. The dominant genus taxon, for methanogens, was Methanosate in TG, while in CK was Methanosarcina. For bacteria, dominant taxa were similar for all groups, which were Solibacillus and Clostridium. Therefore, strain KA3 improved the methane conversion of the substrate. This study could provide a new microbial resource and practical application base for lignin degradation.

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“…In addition, Q e of the solid-state KOH pretreatment decreases with the increase of KOH loading during pretreatment. The negative net value of the chemical/ thermal pretreatment method was widely reported in previous reports, 17,33 which also limits the industrial application of these substrate pretreatment technologies. Recovery techniques (e.g., the recovery of chemical reagents and energy) for pretreatment are urgently needed to reduce the cost of the pretreatment process.…”

Section: Resultsmentioning

confidence: 97%

Solid-State KOH Pretreatment of Corn Straw for Anaerobic Digestion: Methane Yield Enhancement, Potassium Flow Analysis, and Preliminary Economic Assessment

et al. 2019

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Pretreatment has been widely used to improve the anaerobic digestion (AD) efficiency of cellulosic substrates. However, intrinsic shortcomings of traditional pretreatment techniques limit their industrial applications. In this study, a solid-state alkaline pretreatment with KOH to improve the AD efficiency of corn straw was developed, which could largely decrease the chemical needed during pretreatment. In addition, it could be done during the feedstock storage without the need for liquid/solid separation before AD. Furthermore, the potassium added during pretreatment could be preserved in the AD digestate, which could benefit its utilization as a fertilizer. Results showed that the solid-state KOH pretreatment significantly improved the methane yield and AD efficiency that depended on KOH loading. Under 8% KOH load, the maximum methane yield was obtained, which was 31.9% higher than that of the original corn straw. Structural analysis showed that the intact structures (especially lignin) of corn straw were partly destroyed during pretreatment, which improved the AD performance of pretreated corn straw. The added K+ was well preserved in the digestate, more than 76% of which was distributed in the biogas slurry as water-soluble K+. The economy of solid-state KOH pretreatment was strongly associated with the KOH loads during pretreatment as well as with the utilization pattern of the biogas digestate. Only pretreatment with 1% KOH loading showed a positive net value of 0.16 USD/day without considering the value of the AD digestate.

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Simultaneous Pretreatment and Biohydrogen Production from Wheat Straw by Newly Isolated Ligninolytic Bacillus Sp. Strains with Two-Stage Batch Fermentation System

Cited by 20 publications

References 60 publications

Production of Ligninolytic Enzymes from Thermophilic Bacterial Strains Isolated from Palm Oil Wastes

Production of Ligninolytic Enzymes from Thermophilic Bacterial Strains Isolated from Palm Oil Wastes

Enhanced Biogas Production by Ligninolytic Strain Enterobacter hormaechei KA3 for Anaerobic Digestion of Corn Straw

Solid-State KOH Pretreatment of Corn Straw for Anaerobic Digestion: Methane Yield Enhancement, Potassium Flow Analysis, and Preliminary Economic Assessment

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