Simultaneous Multielemental Analysis of the Leaf of Moringa oleifera by Direct Current Arc Optical Emission Spectroscopy

Sharma, Sweta; Shukla, Nidhi; Bharti, Abhi Sarika; Uttam, K. N.

doi:10.1007/s40009-017-0603-0

Cited by 11 publications

(3 citation statements)

References 5 publications

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“…The achieved intensity ratio of the elements Ca, K, Mg, P, S, Fe, Mn, Zn, Na and Se were ranged from 2.7 - 1.7, 1.2 - 1.4, 0.9, 1.1, 1.1, 1.1 - 0.9, 1.0, 0.9, 1.2 and 1.1, respectively which were consistent with those reported in the literature [40]. These results approved that the MOSs are rich in different minerals which are useful to human as food and medicine, indicating their remarkable influence in regulating the level of blood pressure, blood lipids, regulating the stomach, protecting the liver, strengthening bones, generate protein and enhancing the immunity of the body [41]. Furthermore, the existence of Se in the MOSs seeds plays a vital role by protecting from cancer, cardiovascular disease, cognitive decline and thyroid disease.…”

Section: Resultssupporting

confidence: 89%

Elemental analysis of Moringa oleifera seeds by Laser Induced Breakdown Spectroscopy (LIBS) and its anti-cancer and anti-microbial studies

Gondal

2020

Preprint

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16 17 18 1 Abbreviations: MOSs, Moringa oleifera seed; LIBS, Laser Induced Breakdown Spectroscopy; GC-MS ,Gas chromatography-mass spectrometry.Abstract 20 Moringa oleifera plant contains a numerous antioxidants, antibiotics and nutrients (vitamins and minerals) 21 which makes it prospective for diverse biomedical applications. This report investigated the anti-22 cancerous and anti-microbial potential of the Moringa oleifera seeds (MOSs) due to the bioactive 23 components a detail elemental analysis. MOSs in the form of pellets were used in elemental analysis. 24 Alcoholic extraction of were utilized in anti-cancerous and anti-microbial activity.To clarify the anti-25 cancerous and anti-microbial potential of the (MOSs) because of the bioactive components. An elemental 26 analysis was conducted using Laser Induced Breakdown Spectroscopy (LIBS) . The GC-MS used for the 27 validation of the LIBS outcome. Additionally, the anti-cancerous and anti-microbial activity of the MOSs 28 was evaluated. Herein, the human colorectal carcinoma cells (HCT-116) were treated with the seeds 29 aqueous extracts for 48 h and the cell viability plus the DNA nuclear morphology were measured via the 30 MTT assay and DAPI staining. The cell viability of the normal human embryonic stem cells (HEK-293) 31 was also examined after the treatment. Percentage of cell viability and inhibitory concentration (IC 50 ) of 32 both normal and treated cells were determined.The recorded LIBS signal of the MOSs revealed the 33 existence of elements like Ca, K, Mg, P, S, Fe, Mn, Zn, Na and Se (vital for human health). The results of 34 the MTT assay revealed a profound inhibitory action of the MOSs extracts against the HCT-116 cells 35 growth. On top, such extracts did not affect the HEK-293 cells growth, indicating the specificity of the 36 proposed extracts towards cancer cells escalation hindrance. The anti-microbial activity of the ethanolic 37 MOSs extracts was tested on the S. aureus and E. coli bacteria using the Agar well diffusion assay. The 38 observed anti-cancerous and the anti-microbial activities of the MOSs extracts can be attributed to the 39 charisma of various bioactive compounds including the oleic acid, palmitic acid, hexadecenoic acid ethyl 40 ester and D-allose. 3 41 Conclusion: Current observations may contribute towards the development of the MOSs-based 42 biomedicine (organic without any side effects, cheap, plentiful, pure and sustainable) effective for the 43 cancer and bacterial infection cure. 44 45 46 47 Generally, the moringa tree with the scientific name of Moringa oleifera Lam. (MOL) is the part of 48 Moringaceae family [1] . Being originated from the Northern India and Pakistan region alongside the 49 Himalaya Mountains, moringa tree also grows in different parts of African, Asian, and South American 50 tropics [2-4] . In Pakistan, India, Thailand and Philippines the moringa leaves and fruits are usually 51 consumed as the vegetables [5]. In recent times, several countries (Mexico, Hawaii, Cambodia, and the 52Ca...

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Section: Resultssupporting

confidence: 89%

Elemental analysis of Moringa oleifera seeds by Laser Induced Breakdown Spectroscopy (LIBS) and its anti-cancer and anti-microbial studies

Gondal

2020

Preprint

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“…Therefore, a technique is required which is free from sample digestion and should be sensitive, reliable, inexpensive, user friendly, eco-friendly, free from spectral interference and having simultaneous multi elemental capability. One such technique is direct current (d c) arc optical emission spectroscopy and it works on the principle of detection of optical radiation emitted by the sample on excitation by direct current source (Shukla, 2019 andSharma et al, 2017). The direct current arc optical emission spectroscopy allows the sample to be analyzed without sample digestion.…”

Section: Introductionmentioning

confidence: 99%

Elemental Assessment Of momordica charantia by Dc Arc Optical Emission Spectroscopy

Shukla¹,

Uttam²

2021

PLANT ARCHIVES

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This work is intended to research the minerals present in the Momordica charantia by utilizing the Direct Current Arc Optical Emission Spectroscopy (D C Arc OES). Vegetables contain huge amounts of minerals, nutrients, starches, essential amino acids and dietary fibers that are needed for ordinary working of human metabolic cycles. It is consequently important to get the elemental assessment and their concentration in the Momordica charantia. The emission spectrum of the powder sample of the Momordica charantia has been procured in the spectral region 300-900 nm at a resolution of 0.3 nm. The investigation of recorded emission spectrum shows the presence of persistent atomic lines of chromium,

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“…Over the past decade, the LIBS method has broadly been exploited for analyzing chemical elements existing in diverse kinds of specimens. This analytical method has distinguishing properties, like cost effectiveness, real-time measurement, sensitivity, rapidity and in situ elemental analysis [ 18 , 19 , 20 ]. Additionally, this type of analysis is easy, eco-friendly and is less complicated when preparing the sample.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal and In Vitro Cytotoxicity of Moringa oleifera Seed Extract and Its Elemental Analysis Using Laser-Induced Breakdown Spectroscopy

et al. 2020

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In the current study, we present the correlation between the capability of laser-induced breakdown spectroscopy (LIBS) to monitor the elemental compositions of plants and their biological effects. The selected plant, Moringa oleifera, is known to harbor various minerals and vitamins useful for human health and is a potential source for pharmaceutical interventions. From this standpoint, we assessed the antibacterial and in vitro cytotoxicity of the bioactive components present in Moringa oleifera seed (MOS) extract. Detailed elemental analyses of pellets of MOSs were performed via LIBS. Furthermore, the LIBS outcome was validated using gas chromatography–mass spectrometry (GC-MS). The LIBS signal was recorded, and the presence of the essential elements (Na, Ca, Se, K, Mg, Zn, P, S, Fe and Mn) in the MOSs were examined. The bactericidal efficacy of the alcoholic MOS extract was examined against Escherichia coli (E. coli) and Staphylococcus aureus(S. aureus) by agar well diffusion (AWD) assays and scanning electron microscopy (SEM), which depicted greater inhibition against Gram-positive bacteria. The validity and DNA nuclear morphology of human colorectal carcinoma cells (HCT-116) cells were evaluated via an MTT assay and DAPI staining. The MTT assay results manifested a profoundly inhibitory action of MOS extract on HCT116 cell growth. Additionally, MOS extracts produced inhibitory action in colon cancer cells (HCT-116), whereas no inhibitory action was seen using the same concentrations of MOS extract on HEK-293 cells (non-cancerous cells), suggesting that MOS extracts could be non-cytotoxic to normal cells. The antibacterial and anticancer potency of these MOS extracts could be due to the presence of various bioactive chemical complexes, such as ethyl ester and D-allose and hexadecenoic, oleic and palmitic acids, making them an ideal candidate for pharmaceutical research and applications.

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Simultaneous Multielemental Analysis of the Leaf of Moringa oleifera by Direct Current Arc Optical Emission Spectroscopy

Cited by 11 publications

References 5 publications

Elemental analysis of Moringa oleifera seeds by Laser Induced Breakdown Spectroscopy (LIBS) and its anti-cancer and anti-microbial studies

Elemental analysis of Moringa oleifera seeds by Laser Induced Breakdown Spectroscopy (LIBS) and its anti-cancer and anti-microbial studies

Elemental Assessment Of momordica charantia by Dc Arc Optical Emission Spectroscopy

Bactericidal and In Vitro Cytotoxicity of Moringa oleifera Seed Extract and Its Elemental Analysis Using Laser-Induced Breakdown Spectroscopy

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