Simultaneous measurement of serotonin and 5-hydroxyindoleacetic acid in rat brain using a liquid chromatographic method with electrochemical detection

Vries, M. De; Odink, J.

doi:10.1016/0378-4347(91)80087-s

Cited by 20 publications

(12 citation statements)

References 15 publications

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“…In this study, the ratio of the major metabolite to the corresponding monoamine is used as an index of monoamine turnover. For 5-HT metabolism, the ratio of 5-HIAA/5-HT is a good index of apparent serotonin turnover [22]. HPLC analysis revealed that clorgyline pretreatment showed typical pharmacological properties as an irreversible MAO-A inhibitor throughout the period of measurement of morphine-induced hyperlocomotion ( Fig.…”

Section: Discussionmentioning

confidence: 98%

Modification of Morphine-induced Hyperlocomotion and Antinociception in Mice by Clorgyline, a Monoamine Oxidase-A Inhibitor

Kitanaka

Takemura

2006

Neurochem Res

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We evaluated the effects of pretreatment with clorgyline, an irreversible monoamine oxidase (MAO)-A inhibitor, on morphine-induced hyperlocomotion and antinociception. A single administration of morphine (30 mg/kg, i.p.) to male ICR mice induced a hyperlocomotion. ANOVA analysis revealed the statistical significance of the morphine effect on horizontal locomotion and of the clorgyline pretreatment x morphine interaction effect, but not of the effect of clorgyline pretreatment. The initial (5 min after challenge) phase of morphine actions vs. saline challenge appeared as if morphine had a strong inhibitory effect on locomotor activity in combination with different doses of clorgyline. The mice administered with morphine in combination of clorgyline (1 and 10 mg/kg) did not show any stereotypic behaviors. Clorgyline at a dose of 0.1 mg/kg but not other doses tested significantly potentiated morphine-induced antinociception evaluated by tail flick but not hot plate test. During the measurements of locomotor activity and antinociception, clorgyline at doses of 1 and 10 mg/kg significantly inhibited monoamine metabolism through MAO. These results suggest that clorgyline showed an inhibitory effect on morphine-induced hyperlocomotion, but not antinociception, through MAO inhibition. There is not a possibility that clorgyline pretreatment enhanced morphine action on motor activity, resulting in the abnormal behavior from hyperlocomotion to stereotypic movements.

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Section: Discussionmentioning

confidence: 98%

Modification of Morphine-induced Hyperlocomotion and Antinociception in Mice by Clorgyline, a Monoamine Oxidase-A Inhibitor

Kitanaka

Takemura

2006

Neurochem Res

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“…This would indicate that the analyte concentrations are enhanced with sample preparation in ice-cold 0.1 M perchloric acid. This accounts for discrepancies in published data, where tissue samples from rat brains have been prepared in 0.1 M phosphate buffer [14] or in methanol/water [13]. This will also cause problems for fluorescence detection, where samples are left to stand for up to 20 min and treated at high temperatures [16] to be derivatised.…”

Section: Extraction Solventmentioning

confidence: 93%

“…This has led to inconsistency in data e.g. levels of serotonin that vary from g/g of tissue [13] to ng/g [7,14] of tissue in the hypothalamus of rat brains. There have been chromatographic methods that have shown retention times of serotonin ranging from approximately 10 min [1,15] to 30 min [7,16].…”

Section: Introductionmentioning

confidence: 97%

Simple and rapid determination of serotonin and catecholamines in biological tissue using high-performance liquid chromatography with electrochemical detection

Patel

Arundell

Parker

et al. 2005

Journal of Chromatography B

168

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“…There have been studies showing that the use of antioxidants such as Lcysteine and ascorbic acid (though these are electroactive and further complicate an already complex matrix; Thorre et al, 1997) has reduced the degradation of analytes in the biological matrix. The conditions for the repeated assay of prepared biological samples have been determined in various solvents (De Vries and Odink, 1991;Chi et al, 1999) and samples have also been stored in multiple conditions (Chi et al, 1999;Wagner et al, 1982;Schmidt and Ferger, 2004;Ishida et al, 1998). Therefore a great deal of inconsistency in the literature is observed for dealing with sample storage and stability.…”

Section: Introductionmentioning

confidence: 99%

Determination of serotonin, melatonin and metabolites in gastrointestinal tissue using high‐performance liquid chromatography with electrochemical detection

Chau

Patel

2008

Biomedical Chromatography

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In this paper we show a simple isocratic chromatographic method for the detection of serotonin and its precursors and metabolites from various types of gastrointestinal tissue. The paper measures for the first time basal measurements of melatonin in the gastrointestinal tract, which has recently been shown to be released from the musosal lining of the gut. Tissue samples were stable following sample preparation in either 0.1 m perchloric acid or mobile phase. Analysis was carried out using a mobile phase consisting of 10% acetonitrile-90% acetate acid buffer pH 4.0 with 2 mm decane-sulfonic acid sodium salt at a column temperature of 50 degrees C. Electrochemical detection was utilized at a potential of +850 mV vs Ag/AgCl reference electrode at 10 microA full-scale deflection. The detection limit of 5-HT and melatonin was 241 and 308 nm respectively for a 10 microL injection. As a result of the method optimization, total analysis was reduced to 30 min. Accurate responses of the tissue samples following sample preparation could be obtained following a week after storage at -80 degrees C. This method is capable of preparing and analysing of samples from all regions of the gastrointestinal tract.

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Simultaneous measurement of serotonin and 5-hydroxyindoleacetic acid in rat brain using a liquid chromatographic method with electrochemical detection

Cited by 20 publications

References 15 publications

Modification of Morphine-induced Hyperlocomotion and Antinociception in Mice by Clorgyline, a Monoamine Oxidase-A Inhibitor

Modification of Morphine-induced Hyperlocomotion and Antinociception in Mice by Clorgyline, a Monoamine Oxidase-A Inhibitor

Simple and rapid determination of serotonin and catecholamines in biological tissue using high-performance liquid chromatography with electrochemical detection

Determination of serotonin, melatonin and metabolites in gastrointestinal tissue using high‐performance liquid chromatography with electrochemical detection

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