Simultaneous measurement of retinol, α-tocopherol and six carotenoids in human plasma by using an isocratic reversed-phase HPLC method

An optimized and validated RP-HPLC/UV detection method for simultaneous determination of all-trans-Retinol (Vitamin A) and α-Tocopherol (Vitamin E) in human serum: Comparison of different particulate reversed-phase HPLC columns

“…This is due to the short-lived nature of reactive oxygen/nitrogen species formed and lake of well-established methods for measuring oxidative stress status in humans [17]. Although several methods for the determination of these vitamins in plasma and serum have been reported, information on their analytical quality is lacking [18][19][20][21][22][23]. To solve this discordance the accurate measurement of serum concentration of retinol and ␣-tocopherol is an important issue [4].…”

A 6-month randomised controlled trial investigating effects of Mediterranean-style diet and fish oil supplementation on dietary behaviour change, mental and cardiometabolic health and health-related quality of life in adults with depression (HELFIMED): study protocol

“…Plasma samples are aliquoted into amber vials and stored at −80°C until analysis. Methods for extraction of carotenoids and preparation of internal standards, external standards, secondary calibrator and plasma samples are based on Karppi et al [47]. Variations to these methods include the use of argon to evaporate samples prior to reconstitution, and the High Performance Liquid Chromatography (HPLC) model used for analyses is an Agilent 1100 with Kinetex 5 μm C18 100 Å columns (250 × 4.6 mm).…”

Rapid Isocratic HPLC Method and Sample Extraction Procedures for Measuring Carotenoid, Retinoid, and Tocopherol Concentrations in Human Blood and Breast Milk for Intervention Studies

“…thrice extracted), (2) a single extraction (but with a total of 3 mL of hexane and 1 mL hexane containing 1 lg mL -1 tocol), or (3) a modified single extraction performed by freezing the centrifuged samples to aid the decantation process similar to the described method by Karppi et al [26]. Each method was done in triplicate.…”

“…Many methods have been developed for human blood [20,[24][25][26][27]. However, most of these methods require prolonged (30-60 min) run times, which is not ideal for testing hundreds (or thousands) of samples required to monitor VAD programs.…”