Simultaneous LC–MS–MS Analysis of Danshensu, Salvianolic Acid B, and Hydroxysafflor Yellow A in Beagle Dog Plasma, and Application of the Method to a Pharmacokinetic Study of Danhong Lyophilized Powder for Injection

Metabolite identification of tanshinol borneol ester in rats by high‐performance liquid chromatography combined with electrospray ionization quadrupole time‐of‐flight mass spectrometry

“…The deprotonated molecular ion of M5 was produced by breaking of the ester bond in DBZ. The full MS/MS product ion spectrum of M5 showed the characteristic product ions of m / z 179.0304, m / z 135.0402 and m / z 123.0406, which confirmed the structural unit information of DSS (Zhan et al, ). Therefore M5 was considered as DSS, the hydrolyzate of DBZ (Figure S2).…”

Protective effect of Danhong injection on cerebral ischemia–reperfusion injury in rats

“…The main active components of DH are the phenols such as danshensu, protocatechuic aldehyde, salvianolic acid B, rosmarinic acid and the flavonoids such as hydroxysafflor yellow A (Zhan et al, 2008). In accordance with the corresponding quality control standard, the total content of danshensu (molecular formula: C 9 H 10 O 5 ) and protocatechuic aldehyde (molecular formula: C 7 H 6 O 3 ) should not be lower than 0.5 mg when determined by HPLC, and that of the total flavonoids detected by visible spectrophotometry should not be lower than 5.0 mg against rutin (molecular formula: C 27 H 30 O 16 ) as a reference in 1 ml injection (SFDA, 2002).…”

“…Since the former is cold, while the latter is warm in nature according to traditional Chinese Medical theory, these two herbal plants are frequently applied together to achieve synergistic therapeutic efficacy and get rid of the side effect in clinical decoctions or Chinese patent medicines like Danhong injection Zhan et al, 2008).…”

Simultaneous determination of six hydrophilic components in rat plasma after oral administration of Jitai tablet by liquid chromatography–electrospray ionization–tandem mass spectrometry: Application to a pharmacokinetic study

“…Various analytical methods based on liquid chromatography technology have been developed for the determination of the six compounds in biological fluids, respectively [18][19][20][21][22][23][24]. However, these methods have shortens: high limit of quantification, long analysis time, or could not fulfill oral PK study due to the insufficient sensitivity and specificity.…”