Simultaneous identification of bovine and equine DNA in milks and dairy products inferred from triplex TaqMan real-time PCR technique

Guo, Liang; Qian, Jun-Ping; Guo, Yiting; Hai, Xiao; Liu, Guoqiang; Luo, Jian-Xing; Ya, Mei

doi:10.3168/jds.2018-14408

Cited by 30 publications

(18 citation statements)

References 25 publications

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“…DNA from meat and dairy products was isolated with TaKaRa MiniBEST Universal Genomic DNA Extraction Kit, using a modified CTAB method (Guo et al., 2018). DNA concentration and quality were assessed using a NanoDrop 2000c at a wavelength of 260 nm and 280 nm.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous detection of ovine and caprine DNA in meat and dairy products using triplex TaqMan real‐time PCR

Guo¹,

Yu²,

Xu³

et al. 2020

Food Science & Nutrition

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Meat and dairy products provide essential nutrients that support growth, functions, immunity, and general human well-being. Xilingol grassland (203,000 km 2) in China is a natural grazing region, known for the free raising of animals for human consumption and its low pollution levels, resulting in the production of high-quality meat and dairy products from sheep and goat, among others. The average annual output of sheep and goat in Xilingol grassland is more than ten million of animals. Nevertheless, some producers and traders deliberately adulterate meat and dairy products with less expensive substitutes, which unbalances market competition and disregards consumer's interests. Further, product authentication is required to manage health risks and to comply with religious norms. Therefore, the development of efficient approaches for product authentication is essential to ensure the purity of food products. Here, we developed a specific and highly sensitive method to evaluate adulteration of meat and milk from sheep and goat based on DNA detection using real-time PCR with TaqMan technology. Distinct analytical PCR-based methods have been developed for DNA detection in animal-derived products such as meat and dairy

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Section: Methodsmentioning

confidence: 99%

Simultaneous detection of ovine and caprine DNA in meat and dairy products using triplex TaqMan real‐time PCR

Guo¹,

Yu²,

Xu³

et al. 2020

Food Science & Nutrition

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“…A triplex real-time PCR was recently developed for the identification of bovine and equine DNA by employing developed primers and probes. The limits of detection of the method were 0.001 ng for milk and yogurt and 0.005 ng for sour soup and koumiss, respectively [ 128 ].…”

Section: Dairy Productsmentioning

confidence: 99%

Milk Products from Minor Dairy Species: A Review

Faccia

D’Alessandro²,

Summer

et al. 2020

Animals

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Milk processing is one of the most ancient food technologies, dating back around 6000 BC. The majority of dairy products are manufactured from cows, buffaloes, goats, and sheep; their production technologies are mostly standardized and have been widely investigated. Milk and dairy products from minor species are less important under the economic point of view, but they play a fundamental social role in many marginal and poor areas. Due to scarce interest of the dairy industry, their technological characteristics and related issues have been investigated less. Recently, the increasing interest toward ethnic foods and food biodiversity is helping these minor products to emerge from the “darkness” in which they have remained for long time. Some of them are increasingly seen as useful for the valorization of marginal areas, while others are recognized as innovative or healthy foods. The present review aims to resume the most recent knowledge about these less-known dairy products. The first part summarizes the main technological properties of equine, camel, and yak milk with a view to processing. The second is a survey on the related dairy products, both the traditional ones that have been manufactured for a long time and those that have been newly developed by food researchers.

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“…The partial DNA fragments from GM maize or rice were detected in the blood and intestinal contents [ 24 , 25 ]. Guo et al (2018) observed the existence of bovine and equine DNA in milk, yogurt, and other dairy products using one triplex real-time PCR [ 26 ]. Our results also confirmed that the transgenic hLZ DNA and endogenous reference gene could be detected in fresh goat milk samples, and the developed method could be further used for the detection of the transgene content in the goat milk products.…”

Section: Resultsmentioning

confidence: 99%

An Event-Specific Real-Time PCR Method for Measuring Transgenic Lysozyme Goat Content in Trace Samples

Cui

Liu

et al. 2021

Foods

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Lysozymes are used in sterilisation, antisepsis, dairy additives, inflammation, and cancer. One transgenic goat line expressing high levels of human lysozyme (hLZ) in goat milk has been developed in China. Herein, we established an event-specific real-time polymerase chain reaction (real-time PCR) method to detect the transgenic hLZ goat line. The developed method has high specificity, sensitivity and accuracy, and a wide quantitative dynamic range. The limit of detection and limit of quantification was 5 and 10 copies per reaction, respectively. The practical sample analysis results showed that the method could identify and quantify transgenic lysozyme content in trace samples in routine lab analyses. Furthermore, the potential applicability in risk assessment, such as molecular characterisation and gene horizontal transfer, was confirmed. We believe that this method is suitable for the detection of transgenic hLZ goat line and its derivate.

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Simultaneous identification of bovine and equine DNA in milks and dairy products inferred from triplex TaqMan real-time PCR technique

Cited by 30 publications

References 25 publications

Simultaneous detection of ovine and caprine DNA in meat and dairy products using triplex TaqMan real‐time PCR

Simultaneous detection of ovine and caprine DNA in meat and dairy products using triplex TaqMan real‐time PCR

Milk Products from Minor Dairy Species: A Review

An Event-Specific Real-Time PCR Method for Measuring Transgenic Lysozyme Goat Content in Trace Samples

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