Simultaneous high nutritional single cell oil and lipase production by Candida viswanathii

Abstract

Background. Omega fatty acids are a family of polyunsaturated fats associated with several health benefits. Lipases are enzymes with potential application in several food processes such as flavor and aroma, surfactants and formulations for the dairy and bakery industries. In this study, single cell oil and lipase production by Candida viswanathii CCR8137 were evaluated simultaneously from renewable carbon sources under nitrogen limitation. Materials and methods. Enzyme and single cell oil were obtained in submerged cultivations supplemented with triolein, tributyrin, corn oil, sunflower oil, canola oil and olive oil. The effects of glucose on lipid accumulation, fatty acid profile, enzyme production and cell morphology were also evaluated. Results. The highest lipid accumulation (44.5%, w/w) was obtained from triolein, whereas olive oil was the best inducer of lipase synthesis (26.8 U/mL). Nitrogen limiting cultivations were a key parameter for an organic source which showed higher lipid accumulation and enzyme production than the tested inorganic nitrogen source. Glucose was a poor inducer of lipase synthesis, though increased values of lipid accumulation were observed from this carbon source with a maximum of 63.1% (w/w). The fatty acid profile of lipids produced by C. viswanathii CCR8137 showed a high content of omega-9 fatty acid (C18:1 n-9). The addition of glucose to the culture media resulted in the synthesis of essential fatty acids: vaccenic, linolenic and eicosadienoic acids. Conclusion. Therefore, C. viswanathii CCR8137 strain can be considered as an oleaginous yeast able to accumulate high concentrations of intracellular lipids, which are potential additives for food industry applications as well as being able to simultaneously synthesize high yields of lipase.

Production, Biosynthesis, and Commercial Applications of Fatty Acids From Oleaginous Fungi

“…During the cell proliferation stage, cells proliferate and metabolize vigorously, with the nutrients in the medium consumed rapidly. During the fatty acid accumulation stage, the nitrogen source is exhausted but the carbon source is sufficient in the medium, which makes cells stop proliferating for the most part, with the lipid synthesis becoming the dominated metabolic activity ( 31 – 33 ).…”

Valorization of lipid-rich wastewaters: A theoretical analysis to tackle the competition between polyhydroxyalkanoate and triacylglyceride-storing populations

“…Thus, oleaginous yeasts belonging to the genus Candida (OtuF0002) have been widely recorded to use not only hydrophilic but also hydrophobic substrates (i.e. triolein, olive oil, linseed oil) via the ex novo pathway (Dias et al, 2021;Papanikolaou and Aggelis, 2011b) and stand out by their ability to hydrolyze lipids (Theerachat et al, 2017). Fonsecaea (OtuF0005) has not been identified as an oleaginous fungus although it was demonstrated its ability to produce high levels of secretory lipases (Okeke and Gugnani, 1989).…”