Simultaneous frequency and dissipation factor QCM measurements of biomolecular adsorption and cell adhesion

Rodahl, Michael; Höök, Fredrik; Fredriksson, C.; Keller, Craig A.; Krozer, Anatol; Brzezinski, Peter; Voinova, Marina; Kasemo, Bengt Herbert

doi:10.1039/a703137h

Cited by 654 publications

(695 citation statements)

References 30 publications

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“…For soft and viscoelastic layers, the Vogit model was used (Rodahl et al, 1997) to fit the frequency change (Δf ) and energy dissipation (ΔD) simultaneously at multiple overtones to obtain the mass and physical properties (e.g., density, thickness, shear elastic modulus, and viscosity) of the deposited film. In Voigt model, fluid density and fluid viscosity were 1000 kg /m 3 , 0.001 kg /(m·sec), respectively.…”

Section: Characterization By Qcm-dmentioning

confidence: 99%

Deposition behavior of residual aluminum in drinking water distribution system: Effect of aluminum speciation

Zhang

Shi

Zhao

et al. 2016

Journal of Environmental Sciences

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Section: Characterization By Qcm-dmentioning

confidence: 99%

Deposition behavior of residual aluminum in drinking water distribution system: Effect of aluminum speciation

Zhang

Shi

Zhao

et al. 2016

Journal of Environmental Sciences

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“…The QCM-D instrument used in the present work (Q-Sense AB, Göteborg, Sweden) simultaneously monitors a second important parameter, namely the damping, or the dissipation factor, D, of the oscillator. The dissipation measurements add qualitative and usually also quantitative information about the viscoelastic properties of the adsorbed layer (see, e.g., 14, 15) and allow for theoretical modeling of the QCM-D response (25,26). In brief, the dissipation is generally high if the adsorbed film has a high viscous component.…”

Section: The Qcm-d Techniquementioning

confidence: 99%

Protein Adsorption on Supported Phospholipid Bilayers

Glasmästar

Larsson

Höök

et al. 2002

Journal of Colloid and Interface Science

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293

286

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Quartz crystal microbalance with dissipation (QCM-D) measurements were used to investigate the adsorption of human fibrinogen, human serum albumin, bovine hemoglobin, horse heart cytochrome c, human immunoglobulin (hIgG), and 10% fetal bovine serum on supported bilayers of egg-phosphatidylcholine (eggPC) lipids. For comparison the adsorption of fibrinogen and hIgG to eggPC bilayers was also studied with surface plasmon resonance (SPR). The supported bilayers were formed in situ by vesicle adhesion and spontaneous fusion onto a SiO 2 surface. The supported lipid bilayer is highly protein resistant: The irreversible adsorption measured with the QCM-D technique was below the detection level, while reversible protein adsorption was detected for all the proteins in the range 0.3-4% of the saturation coverage on a hydrophobic thiol monolayer on gold. The adsorbed amounts were slightly higher for the SPR measurements. Possible mechanisms for the protein resistance of eggPC bilayers are briefly discussed. C 2002 Elsevier Science

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“…A detailed description of the technique and its basic principles can be found elsewhere. 24 Goldcoated quartz sensors (Q-sense AB, Sweden) were coated with an additional PDMS layer in the same manner as described for OWLS waveguide chips. Solutions were supplied into the QCM-D chamber using an Ismatec peristaltic pump IPC-N 4 at a flow rate of 0.2 mL•min -1 .…”

Section: Quartz Crystal Microbalance With Dissipation (Qcm-d)mentioning

confidence: 99%

Structural and Mechanical Properties of Thin Films of Bovine Submaxillary Mucin versus Porcine Gastric Mucin on a Hydrophobic Surface in Aqueous Solutions

et al. 2016

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The structural and mechanical properties of thin films generated from two types of mucins, namely bovine submaxillary mucin (BSM) and porcine gastric mucin (PGM) in aqueous environment were investigated with several bulk and surface analytical techniques. Both mucins generated hydrated films on hydrophobic polydimethylsiloxane (PDMS) surfaces from 2 spontaneous adsorption arising from their amphiphilic characteristic. But, BSM formed more elastic films than PGM at neutral pH condition. This structural difference was manifested from the initial film formation processes to the responses to shear stresses applied to the films.Acidification of environmental pH led to strengthening the elastic character of BSM films with increased adsorbed mass, whereas an opposite trend was observed for PGM films. We propose that this contrast originates from that negatively charged motifs are present for both the central and terminal regions of BSM molecule, whereas a similar magnitude of negative charges is localized at the termini of PGM molecule. Given that hydrophobic motifs acting as anchor are also localized in the terminal region, electrostatic repulsion between anchoring units of PGM molecules on nonpolar PDMS surface leads to weakening of the mechanical integrity of the films.

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Simultaneous frequency and dissipation factor QCM measurements of biomolecular adsorption and cell adhesion

Cited by 654 publications

References 30 publications

Deposition behavior of residual aluminum in drinking water distribution system: Effect of aluminum speciation

Deposition behavior of residual aluminum in drinking water distribution system: Effect of aluminum speciation

Protein Adsorption on Supported Phospholipid Bilayers

Structural and Mechanical Properties of Thin Films of Bovine Submaxillary Mucin versus Porcine Gastric Mucin on a Hydrophobic Surface in Aqueous Solutions

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