Simultaneous Fluorometric Resolution of Pyridoxal, Pyridoxamine and 4-Pyridoxic Acid Using Chemometric Methods

Abstract

An alternatively minimizing covariant matrix error (AMCME) algorithm, newly proposed by the present authors, was applied to the simultaneous fluorometric determination of pyridoxal, pyridoxamine and 4-pyridoxic acid without loss of sensitivity. The experimental results illustrate that the profiles of spectra and concentration can be accurately resolved using the AMCME algorithm with a high sensitivity and stable repeatability. That is to say, the closely overlapping problem of the spectra could be resolved owing to the characteristic features of the AMCME algorithm.

Cetyltrimethylammonium micelles enhance the sensitivity of ssDNA-based electrochemical sensor for the determination of pyridoxol

“…Determination methods were reported, such as high-performance liquid chromatography, 13 ow injection-solid phase spectrophotometry, 14 and uorescence spectrometry. [15][16][17] Among voltammetric determinations, Lindquist 18 rst reported the voltammetric measurement of pyridoxine using a carbon paste electrode in 1975; subsequently, modied electrodes were developed to improve sensitivity and selectivity. [19][20][21][22][23][24][25][26][27][28][29] Unfortunately, most of the modied electrodes possess high electrooxidation potential, resulting in interference signals in coexisting systems.…”