Simultaneous Five Cell-Lineage Flow Cytometric Analysis System for Detection of Leucocyte Antibodies

Matsuyama, Nobuki; Kojima, Yoshitaka; Hirayama, Fumiya; Yasui, Kazuta; Taniue, Atsuko; Fukumori, Yasuo; Yoshimura, Keiji; Tabata, Nobutada; Sakata, Naomi; Tani, Yoshihiko; Shibata, Hirotoshi

doi:10.3925/jjtc.53.477

Cited by 10 publications

(26 citation statements)

References 25 publications

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“…In particular, HNA-3a is present on lymphocytes and platelets (11,19). For example, HNA-3, HNA-4, and HNA-5 are present on WBCs other than neutrophils.…”

Section: Discussionmentioning

confidence: 99%

“…In Matsuyama's protocol (11), the samples were divided into two wells midway through the assay. In our protocol, whole blood was not used even at the sensitization step.…”

Section: Discussionmentioning

confidence: 99%

“…While the anti-platelet Ab1 control sera reacted with platelets alone, anti-HLA Ab1 control sera reacted with all three cell types (Figs. This may be because some human neutrophil antigens (HNAs) (e.g., HNA-3a) are also expressed on platelets or on lymphocytes (11,19). Although we expected that anti-neutrophil Ab1 control sera might react with neutrophils alone, two cases from one infant with autoimmune neutropenia reacted strongly with platelets also (Fig.…”

Section: Comprehensive Reaction Patterns Of Positive Control Sera Conmentioning

confidence: 98%

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A Novel Flow Cytometric Method for the Simultaneous Detection of Antibodies Against Platelet, Lymphocyte, And Neutrophil

Suh

Won

2016

Cytometry Part B Clinical

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“…In particular, HNA-3a is present on lymphocytes and platelets (11,19). For example, HNA-3, HNA-4, and HNA-5 are present on WBCs other than neutrophils.…”

Section: Discussionmentioning

confidence: 99%

“…In Matsuyama's protocol (11), the samples were divided into two wells midway through the assay. In our protocol, whole blood was not used even at the sensitization step.…”

Section: Discussionmentioning

confidence: 99%

Section: Comprehensive Reaction Patterns Of Positive Control Sera Conmentioning

confidence: 98%

See 1 more Smart Citation

A Novel Flow Cytometric Method for the Simultaneous Detection of Antibodies Against Platelet, Lymphocyte, And Neutrophil

Suh

Won

2016

Cytometry Part B Clinical

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“…The antigen specificities of the maternal Hit antibody were also examined by five‐cell‐lineage analysis using a flow cytometer (FACSCalibur, BD Biosciences, San Jose, CA) 28,29 …”

Section: Methodsmentioning

confidence: 99%

Neonatal alloimmune thrombocytopenia caused by an antibody specific for a newly identified allele of human platelet antigen‐7

et al. 2010

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“…When fluorescence activity was detected, data were obtained from three cell subsets (lymphocytes, granulocytes and monocytes) using gating definition, as previously described. [19][20][21] Immunoblotting Tissue lysates were prepared from the injured arteries for immunoblotting analysis. Identical amounts of protein were separated by SDS-PAGE and blotted onto nitrocellulose membranes.…”

Section: Flow Cytometrymentioning

confidence: 99%

Combination of amlodipine and atorvastatin synergistically reduces leukocyte recruitment to mechanically injured mouse femoral artery

et al. 2011

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Recent studies have demonstrated a potential synergistic effect of the combination of amlodipine with atorvastatin to reduce acute inflammation. The intraluminal wire injury of the mouse femoral artery induced significant leukocyte recruitment to the injured area and oxidative stress within 24 h. Administration of low-dose amlodipine (0.5 mg kg À1 per day) or atorvastatin (1 mg kg À1 per day) alone for 7 days failed to modulate leukocyte adhesion, whereas their co-administration for 7 days significantly inhibited leukocyte adhesion. Moreover, flow cytometric analysis showed that injury-induced oxidative stress and CD11b expression in three leukocyte fractions were elevated after injury and then reduced after the co-administration. Next, adoptive transfer of mononuclear cells (MNCs) was performed and MNCs were harvested from mice after wire injury exhibited adhesion to the recipient injured artery. Furthermore, the co-administration of low-dose atorvastatin and amlodipine to MNCs or the vasculature reduced the recruitment of MNCs to the injured artery. Our findings indicate that amlodipine and atorvastatin synergistically inhibit vascular inflammation. The underlying mechanisms of their effect involve, at least in part, stabilizing oxidative stress at the point of injury, suggesting the clinical efficacy of this drug combination for the treatment of vascular diseases.

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Simultaneous Five Cell-Lineage Flow Cytometric Analysis System for Detection of Leucocyte Antibodies

Cited by 10 publications

References 25 publications

A Novel Flow Cytometric Method for the Simultaneous Detection of Antibodies Against Platelet, Lymphocyte, And Neutrophil

A Novel Flow Cytometric Method for the Simultaneous Detection of Antibodies Against Platelet, Lymphocyte, And Neutrophil

Neonatal alloimmune thrombocytopenia caused by an antibody specific for a newly identified allele of human platelet antigen‐7

Combination of amlodipine and atorvastatin synergistically reduces leukocyte recruitment to mechanically injured mouse femoral artery

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