A Novel Flow Cytometric Method for the Simultaneous Detection of Antibodies Against Platelet, Lymphocyte, And Neutrophil

Abstract

Our method could enhance both interpretation accuracy of the diagnostic test results and laboratory efficiency. As an increased reaction with platelets is commonly due to anti-HLA Abs, the reactivity of the lymphocytes from the same donor should also be tested, which can be routinely done by using our protocol. © 2016 International Clinical Cytometry Society.

Issue Highlights—March 2018

“…The paper by Puñet‐Ortiz et al is a good example of introduction of flow‐based measurements of receptor occupancy in the clinical practice, to optimize the treatment of patients with multiple sclerosis. A method based on fluorescence quantitative comparisons aimed at the simultaneous detection of platelet, lymphocyte, and neutrophil antibodies using the Mean Fluorescence Intensity (MFI) ratio is also described here by Suh et al .…”