Simultaneous Expression of Abiotic Stress Responsive Transcription Factors, AtDREB2A, AtHB7 and AtABF3 Improves Salinity and Drought Tolerance in Peanut (Arachis hypogaea L.)

Pruthvi, V.; Rama, Nicolas; Nataraja, K.

doi:10.1371/journal.pone.0111152

Cited by 80 publications

(38 citation statements)

References 75 publications

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“…We show that the expression levels of ATHB7 , PP2C , and RD20 , which are thought to be regulated by the cleaved form of bZIP17, were reduced in bzip17 , under high‐salt stress, after salt acclimation (Figure S8). These genes are regulated by both salt and drought stress, or by ABA treatment (Takahashi et al ; Zhang and Gan ; Pruthvi et al ). Therefore, we speculate that bZIP17 is associated with salt acclimation memory, via upregulating its target genes.…”

Section: Discussionmentioning

confidence: 99%

Basic‐leucine zipper 17 and Hmg‐CoA reductase degradation 3A are involved in salt acclimation memory in Arabidopsis

Lin

Yan

Kang

et al. 2019

JIPB

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Salt acclimation, which is induced by previous salt exposure, increases the resistance of plants to future exposure to salt stress. However, little is known about the underlying mechanism, particularly how plants store the “memory” of salt exposure. In this study, we established a system to study salt acclimation in Arabidopsis thaliana. Following treatment with a low concentration of salt, seedlings were allowed to recover to allow transitory salt responses to subside while maintaining the sustainable effects of salt acclimation. We performed transcriptome profiling analysis of these seedlings to identify genes related to salt acclimation memory. Notably, the expression of Basic‐leucine zipper 17 (bZIP17) and Hmg‐CoA reductase degradation 3A (HRD3A), which are important in the unfolded protein response (UPR) and endoplasmic reticulum‐associated degradation (ERAD), respectively, increased following treatment with a low concentration of salt and remained at stably high levels after the stimulus was removed, a treatment which improved plant tolerance to future high‐salinity challenge. Our findings suggest that the upregulated expression of important genes involved in the UPR and ERAD represents a “memory” of the history of salt exposure and enables more potent responses to future exposure to salt stress, providing new insights into the mechanisms underlying salt acclimation in plants.

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Section: Discussionmentioning

confidence: 99%

Basic‐leucine zipper 17 and Hmg‐CoA reductase degradation 3A are involved in salt acclimation memory in Arabidopsis

Lin

Yan

Kang

et al. 2019

JIPB

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“…The other notable example of a gene with high condition count (condition count = 8) is AT2G46680 (ATHB7), a member of the homeodomain-leucine zipper family (HD-ZIP). ATHB7 was already shown to confer salt and drought tolerance to plants (Olsson et al 2004;Mishra et al 2012;Pruthvi et al 2014) and act as a negative regulator for plant growth (Olsson et al 2004). In contrast, its functional role for other stress responses is less well characterized.…”

Section: Consreg Successfully Identified Known Tfs That Regulate Abiomentioning

confidence: 99%

Prediction of condition-specific regulatory maps inArabidopsisusing integrated genomic data

Song

Lee

Akter

et al. 2019

Preprint

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Recent advances in genomic technologies have generated large-scale protein-DNA interaction data and open chromatic regions for multiple plant species. To predict condition specific gene regulatory networks using these data, we developed the Condition Specific Regulatory network inference engine (ConSReg), which combines heterogeneous genomic data using sparse linear model followed by feature selection and stability selection to select key regulatory genes. Using Arabidopsis as a model system, we constructed maps of gene regulation under more than 50 experimental conditions including abiotic stresses, cell type-specific expression, and stress responses in individual cell types. Our results show that ConSReg accurately predicted gene expressions (average auROC of 0.84) across multiple testing datasets. We found that, (1) including open chromatin information from ATAC-seq data significantly improves the performance of ConSReg across all tested datasets; (2) choice of negative training samples and length of promoter regions are two key factors that affect model performance. We applied ConSReg to Arabidopsis single cell RNA-seq data of two root cell types (endodermis and cortex) and identified five regulators in two root cell types. Four out of the five regulators have additional experimental evidence to support their roles in regulating gene expression in Arabidopsis roots. By comparing regulatory maps in abiotic stress responses and cell type-specific experiments, we revealed that transcription factors that regulate tissue levels abiotic stresses tend to also regulate stress responses in individual cell types in plants.

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“…2,7,11,[65][66][67] It has also been reported recently that ectopic expression of stress specific transcription factors in combination aids in combating multiple abiotic stresses. [68][69][70] Large numbers of review articles have compiled the relevance of multiple upstream regulatory as well as downstream functional genes underlying a wide array of drought tolerance traits in imparting abiotic stress tolerance ( Table 2). There is a well defined and tightly regulated signaling network starting from stress signal perception up to downstream functional gene activation, and the genes which regulate their activity, whichever be the tolerance mechanism.…”

Section: Target Genes To Manipulate Cellular Tolerancestress Responsimentioning

confidence: 99%

Emerging tools, concepts and ideas to track the modulator genes underlying plant drought adaptive traits: An overview

Nataraja

2016

Plant Signaling & Behavior

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Crop vulnerability to multiple abiotic stresses is increasing at an alarming rate in the current global climate change scenario, especially drought. Crop improvement for adaptive adjustments to accomplish stress tolerance requires a comprehensive understanding of the key contributory processes. This requires the identification and careful analysis of the critical morpho-physiological plant attributes and their genetic control. In this review we try to discuss the crucial traits underlying drought tolerance and the various modes followed to understand their molecular level regulation. Plant stress biology is progressing into new dimensions and a conscious attempt has been made to traverse through the various approaches and checkpoints that would be relevant to tackle drought stress limitations for sustainable crop production.

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Simultaneous Expression of Abiotic Stress Responsive Transcription Factors, AtDREB2A, AtHB7 and AtABF3 Improves Salinity and Drought Tolerance in Peanut (Arachis hypogaea L.)

Cited by 80 publications

References 75 publications

Basic‐leucine zipper 17 and Hmg‐CoA reductase degradation 3A are involved in salt acclimation memory in Arabidopsis

Basic‐leucine zipper 17 and Hmg‐CoA reductase degradation 3A are involved in salt acclimation memory in Arabidopsis

Prediction of condition-specific regulatory maps inArabidopsisusing integrated genomic data

Emerging tools, concepts and ideas to track the modulator genes underlying plant drought adaptive traits: An overview

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