Emerging tools, concepts and ideas to track the modulator genes underlying plant drought adaptive traits: An overview

Ms, Parvathi; Nataraja, K.

doi:10.1080/15592324.2015.1074370

Cited by 8 publications

(4 citation statements)

References 126 publications

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“…Drought, flooding, extreme temperatures conditions (cold, heat and frost), salinity and mineral toxicity are among the main abiotic stresses that negatively affect growth, development and yield of important crops. The knowledge about the physiology, biochemistry and molecular responses involved in abiotic stresses contributes to the discovery of new genes and signaling networks that plants use to cope with these challenges, and it is pivotal for the development of new crop varieties with enhanced tolerance to stress 23 24 25 26 .…”

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confidence: 99%

Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis

Martins

Mafra

Souza

et al. 2016

Sci Rep

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Real-time PCR (RT-qPCR) expression analysis is a powerful analytical technique, but reliable results depend on the use of stable reference genes for proper normalization. This study proposed to test the expression stability of 13 candidate reference genes in Setaria viridis, a monocot species recently proposed as a new C4 model plant. Gene expression stability of these genes was assayed across different tissues and developmental stages of Setaria and under drought or aluminum stress. In general, our results showed Protein Kinase, RNA Binding Protein and SDH as the most stable genes. Moreover, pairwise analysis showed that two reference genes were sufficient to normalize the gene expression data under each condition. By contrast, GAPDH and ACT were the least stably expressed genes tested. Validation of suitable reference genes was carried out to profile the expression of P5CS and GolS during abiotic stress. In addition, normalization of gene expression of SuSy, involved in sugar metabolism, was assayed in the developmental dataset. This study provides a list of reliable reference genes for transcript normalization in S. viridis in different tissues and stages of development and under abiotic stresses, which will facilitate genetic studies in this monocot model plant.

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mentioning

confidence: 99%

Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis

Martins

Mafra

Souza

et al. 2016

Sci Rep

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“…Stress perception is the first stage of drought stress response, to sense water deficit in the soil. The major components of stress sensing consists various kinds of cell surface receptors such as RLKs (Receptor-Like Kinases), G-Protein-Coupled Receptors (GPCRs), ion channel-linked receptors, and two-component histidine kinase receptors (Bhargava & Sawant 2013, Parvathi & Nataraja 2016. RLKs receptors like BR1 (Brassinosteroid Receptor 1) transduce stress signal by receptor internalization (Chae et al 2009).…”

Section: Key Plant Products and Common Mechanisms Utilized By Plants mentioning

confidence: 99%

Key plant products and common mechanisms utilized by plants in water deficit stress responses

Moradi

2016

Bot. Sci.

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Plants are always exposed to fluctuation of environmental factors. Water stress is one of the main abiotic factors limiting plant growth and development on most areas of the world. Declining available water triggers various adaptive processes to cope with water deficit stress. These mechanisms can be categorized into escape, avoidance and tolerance strategies. Avoidance mechanisms are employed to maintain the balance between water uptake and water loss, while tolerance mechanisms are trying to keep the plant functions the same level as unstressed level. Major molecular mechanisms attributed as tolerance strategies include osmotic adjustment, Reactive Oxygen Species (ROS) scavenging, cellular components protection membrane lipid changes and hormone inductions. Huge number of metabolites contributes to these diverse mechanisms, but broadly can be classified into amino acids, carbohydrates, lipids, proteins and secondary metabolites. In this review, the role of these compounds is discussed in the contributed mechanisms against water deficit stress. Identification of these substances (key plant products) and related biochemical processes of drought tolerance might help plant breeders and researchers in different ways such as shortening and facilitating selection procedures in plant breeding programs, improving drought tolerance of sensitive varieties either by transferring related genes or simply exogenous application.

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“…Plant response to drought stress is a complex quantitative trait, controlled by many genes (Tuberosa 2012). Among the various adaptive traits, cellular tolerance is considered as a critical comprehensive trait that minimises damage to cellular structures and metabolism (Parvathi & Nataraja 2016). The level of tolerance to abiotic stress depends on induction of various adaptive pathways, such as sensing of the stress signal, signal transduction and subsequent activation of repair and/or acclimation strategies, depending on the nature and intensity of the stress.…”

Section: Introductionmentioning

confidence: 99%

Transgenic tobacco plants constitutively expressing peanut BTF3 exhibit increased growth and tolerance to abiotic stresses

et al. 2017

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Abiotic stresses limit crop growth and productivity worldwide. Cellular tolerance, an important abiotic stress adaptive trait, involves coordinated activities of multiple proteins linked to signalling cascades, transcriptional regulation and other diverse processes. Basal transcriptional machinery is considered to be critical for maintaining transcription under stressful conditions. From this context, discovery of novel basal transcription regulators from stress adapted crops like peanut would be useful for improving tolerance of sensitive plant types. In this study, we prospected a basal transcription factor, BTF3 from peanut (Arachis hypogaea L) and studied its relevance in stress acclimation by over expression in tobacco. AhBTF3 was induced under PEG-, NaCl-, and methyl viologen-induced stresses in peanut. The constitutive expression of AhBTF3 in tobacco increased plant growth under non stress condition. The transgenic plants exhibited superior phenotype compared to wild type under mannitol- and NaCl-induced stresses at seedling level. The enhanced cellular tolerance of transgenic plants was evidenced by higher cell membrane stability, reactive oxygen species (ROS) scavenging activity, seedling survival and vigour than wild type. The transgenic lines showed better in vitro regeneration capacity on growth media supplemented with NaCl than wild type. Superior phenotype of transgenic plants under osmotic and salinity stresses seems to be due to constitutive activation of genes of multiple pathways linked to growth and stress adaptation. The study demonstrated that AhBTF3 is a positive regulator of growth and stress acclimation and hence can be considered as a potential candidate gene for crop improvement towards stress adaptation.

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Emerging tools, concepts and ideas to track the modulator genes underlying plant drought adaptive traits: An overview

Cited by 8 publications

References 126 publications

Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis

Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis

Key plant products and common mechanisms utilized by plants in water deficit stress responses

Transgenic tobacco plants constitutively expressing peanut BTF3 exhibit increased growth and tolerance to abiotic stresses

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