Simultaneous estimation of captopril and hydrochlorothiazide in combined dosage forms

Abstract

Aim: This work deals with the simultaneous determination of captopril (CAP) and hydrochlorothiazide (HZ) in twocomponent solid dosage form, without prior separation, by three different techniques (simultaneous equation, absorbance ratio method, and first-order derivative method). Materials and Methods: This work was carried out on Shimadzu electron UV1800 double-beam UV-Visible spectrophotometer. The absorption spectra of reference and test solutions were carried out in 1 cm matched quartz cell over the range of 200-400 nm. Methanol and distilled water are used as solvent. Results: The first method is the application of simultaneous equation. Where the linearity ranges for both the drugs were 5-35 µg/ml. The second method is the determination of ratio of absorbance at 271 nm, the maximum absorption of HZ and isobestic wavelength 209 nm, the linearity ranges for both the drugs were 10-120 µg/ml The third method is the first-order derivative method, where the CAP shows wavelength at 222 nm and HZ shows at 340 nm, and the linearity ranges for CAP and HZ were 1-30 µg/ ml and 1-40 µg/ml, respectively. The proposed procedures were successfully applied for the simultaneous determination of both the drugs in commercial tablet preparation. The validity of the proposed methods was assessed by applying the standard addition technique where the percentage recovery of the added standard was found to be 99.52±0.214 and 99.00±0.165 using the simultaneous equation method, 99.76±0.684 and 99.58±0.279 using the graphical absorbance ratio method, and 99.45±0295 and 99.21±0.678 using first derivative method, for CAP and HZ, respectively. Conclusion: The proposed procedures are rapid, simple, require no preliminary separation steps, and can be used for routine analysis of both drugs in quality control laboratories.

Circular Dichroism Spectroscopy: A Facile Approach for Quantitative Analysis of Captopril and Study of Its Degradation

“…Captopril in two different pharmaceutical dosage forms was determined by the proposed methods and the reference method. 48 The results of the proposed method were compared with those of the reference method using point and interval hypothesis tests 49 (Table 4). As the calculated t -(paired) and F -values at the 95% confidence level were less than the tabulated ones, it was concluded that no significant difference among the two methods was observed.…”

“…Captopril in two different pharmaceutical dosage forms was determined by the proposed methods and the reference method . The results of the proposed method were compared with those of the reference method using point and interval hypothesis tests (Table ).…”

H-point Standard Addition Method for the Simultaneous Spectrophotometric Determination of Captopril and Hydrochlorothiazide in Pharmaceutical Formulations

“…The current method was compared with three previously reported spectrophotometric methods based on chemometric approaches (Table 9) [20] . When comparing the methods, it was found that the current method could detect low concentrations of cap and Hcz, making it more suitable for the determination of cap and Hcz in biological samples.…”

“…When comparing the methods, it was found that the current method could detect low concentrations of cap and Hcz, making it more suitable for the determination of cap and Hcz in biological samples. Method HPSAM Simultanous equation [20] Q-analysis method [20] First derivative method [20] λ max , nm 211.…”

Spectrophotometric Indirect Determination of Captopril through Redox Reaction with n-bromosuccinimide and RB dye in Pharmaceutical Products

“…Sensitivity is another important characteristic of molecular absorption technique. Besides, the large linear concentration ranges make the method interesting and versatile for routine analysis of drugs in quality control laboratories (Rao, et al, 2012). Most of the reported methods were based on the redox reaction of captopril with an excess of an oxidizing agent (El-Didamony and Erfan, 2010; Skowron and Ciesielski, 2011).…”