H-point Standard Addition Method for the Simultaneous Spectrophotometric Determination of Captopril and Hydrochlorothiazide in Pharmaceutical Formulations

Abstract

Simultaneous spectrophotometric determination of captopril and hydro-chlorothiazide in pharmaceutical formulations by the H-point standard addition method (HPSAM) is described. Absorbance at 211.5 and 230.3 nm was monitored upon the addition of standard solutions of captopril. The results showed that in prepared mixtures, captopril and hydrochlorothiazide can be determined simultaneously at concentration ratios varying from 1.0:0.8 to 2.5:1.5 μg/mL, respectively. Percentage recovery was found to be 95.33–104.37% for captopril and 96.8–105% for hydrochlorothiazide, with a relative standard deviation (RSD) of 2.46%. The method was successfully used to evaluate the antihypertensive captopril drug in a binary combination of hydrochlorothiazide in real samples with high precision and accuracy within the recovery percentage.

Fast New Method for Estimation of Captopril in Pure and Pharmaceutical Preparation by Reaction with Ammonium Ce (IV) Sulfate in Acid Medium

“…Captopril may cause side effects such as coughing [18]. Captopril has been quantified by more than one analytical method, including spectrophotometry [19][20][21][22], flow injection methods [23][24][25][26][27][28], RP-HPLC [29,30], and HPLC [31][32][33]. Some of these methods are not simple for routine analysis, requiring expensive or complex tools.…”

Perfluorosulfonic Acid Membranes Modified with Polyaniline and Hydrothermally Treated for Potentiometric Sensor Arrays for the Analysis of Combination Drugs

“…Complex sample preparation can lead to a decrease in the analysis accuracy. The analysis of combination drugs without pre-separation and the derivatization of components can be performed using UV spectrophotometry with chemometrics [ 13 , 14 , 15 ]. A low peak resolution of analytes and an additivity rule violation are compensated for by multidimensional mathematical methods [ 14 , 15 ].…”

“…A low peak resolution of analytes and an additivity rule violation are compensated for by multidimensional mathematical methods [ 14 , 15 ]. This approach is mainly used for the analysis of preparations containing two active ingredients, since an increase in the number of analytes increases the determination errors [ 13 , 14 , 15 , 16 ].…”