Simultaneous enzymatic wheat starch saccharification and fermentation to lactic acid by Lactococcus lactis

Hofvendahl, Karin; Åkerberg, Christina; Zacchi, Guido; Hahn‐Hägerdal, Bärbel

doi:10.1007/s002530051503

Cited by 43 publications

(16 citation statements)

References 12 publications

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“…However, such bioconversion (bacterial fermentation) cannot be directly applied to cellulose and lignocelluloses. 5,6 that lactic acid is a typical base-catalyzed product of carbohydrates. 12,13 Our previous studies on the conversion of carbohydrate biomass to high-value products have demonstrated that lactic acid can be formed by the hydrothermal reaction of carbohydrates without the addition of a catalyst.…”

Section: Introductionmentioning

confidence: 99%

Hydrothermal conversion of carbohydrate biomass to lactic acid

et al. 2010

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We investigated the hydrothermal conversion of the carbohydrates including glucose, cellulose, and starch to lactic acid using NaOH and Ca(OH) 2 as alkaline catalysts. Both catalysts significantly promoted the lactic acid formation. The highest yield of lactic acid from glucose was 27% with 2.5 M NaOH and 20% with 0.32 M Ca(OH) 2 at 300 C for 60 s. The lactic acid yields from cellulose and starch were comparable with the yield from glucose with 0.32 M Ca(OH) 2 at 300 C, but the reaction time in the case of cellulose was 90 s. The mechanism of lactic acid formation from glucose was discussed by identifying the intermediate products. Lactic acid may be formed via the formation of aldoses of two to four carbons including aldose of three carbons, which are all formed by reverse aldol condensation and double bond rule of hexose. This implies that carbon-carbon cleavage occurs at not only C 3 AC 4 but also at C 2 AC 3 .

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Section: Introductionmentioning

confidence: 99%

Hydrothermal conversion of carbohydrate biomass to lactic acid

et al. 2010

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“…need complete hydrolysis to glucose which can be performed by separate hydrolysis and fermentation (SHF) [25] or in line with fermentation (simultaneous saccharification and fermentation -SSF) [9,20,22]; the simultaneous saccharification and fermentation could be solved by co-fermentation as well by joint use of an amylolytic fungus and a lactic acid bacterium [24].…”

Section: Introductionmentioning

confidence: 99%

Investigation and modeling of lactic acid fermentation on wheat starch via SSF, CHF and SHF technology

Hetényi¹,

Németh²,

Sevella³

2011

Per. Pol. Chem. Eng.

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Starch based lactic acid fermentation technology was examined and optimized using a non-amylolitic, mesophilic lactic acid bacterium. Comparing simultaneous saccharification and fermentation (SSF) and separate hydrolysis and fermentation (SHF) technologies several issues arose and applying the two techniques needed several compromises concerning the overall process time. A combined hydrolysis and fermentation method was developed which incorporate the advantages of SSF and SHF technologies: applying a time delay in inoculation and cutting down hydrolysis time before fermentation, an optimal inoculation and high efficiency was achieved by kinetic model aided experimental work. Experimental verification of the model gave an excellent productivity result: 4.32 g L −1 h −1 calculated with only the fermentation time, and 2.88 g L −1 h −1 calculated with the overall time of the two processes. With this method, hydrolysis and fermentation time was successfully reduced, enhancing lactic acid productivity and depressing production cost of this low-value chemical.

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“…Starch can be enzymatically hydrolyzed to glucose and further fermented to lactic acid by lactic acid bacteria [31]. Therefore, presence of lactic acid in our standard reactor suggested starch fermentation and high consumption rate of the starch in the reactor [32]. Acetic acid was initially chosen to lower the pH to 4.0 to ensure sterility, however this led to its accumulation in the reactor and resultant pH drop issues.…”

Section: State Of the Standard Reactormentioning

confidence: 99%

Effect of Lactate and Starter Inoculum on Biogas Production from Fresh Maize and Maize Silage

Satpathy¹,

Steinigeweg²,

Siefert³

et al. 2017

AiM

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Lactate is a key intermediate during anaerobic digestion of carbohydrates; however, it fails to receive significant consideration in biogas plants. We examined the influence of lactic acid on biogas production. Two commonly used feeds, fresh maize and maize silage, were selected as substrates due to their difference in lactic acid contents. Additionally, inocula from an agriculture-based biogas plant, a waste water treatment plant and a standardised laboratory reactor were selected to investigate the impact of starter culture on the process. Experiments demonstrated increased total biogas yield of up to 45% in the lactate-rich maize silage over the lactate-devoid fresh maize, but only in cases where the starting inocula had been previously exposed to lactic acid. Our findings suggest lactic acid is a significant intermediate in biogas production and merits consideration. Additionally, the ability of the starter inoculum to utilize lactic acid is an important factor in process optimization and enhanced biogas production.

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Simultaneous enzymatic wheat starch saccharification and fermentation to lactic acid by Lactococcus lactis

Cited by 43 publications

References 12 publications

Hydrothermal conversion of carbohydrate biomass to lactic acid

Hydrothermal conversion of carbohydrate biomass to lactic acid

Investigation and modeling of lactic acid fermentation on wheat starch via SSF, CHF and SHF technology

Effect of Lactate and Starter Inoculum on Biogas Production from Fresh Maize and Maize Silage

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