Simultaneous engineering of nanofillers and patterned surface macropores of graphene/hydroxyapatite/polyetheretherketone ternary composites for potential bone implants

Huang, Zhihuan; Wan, Yizao; Zhu, Xiangbo; Zhang, Peibiao; Yang, Zhiwei; Yao, Fanglian; Luo, Honglin

doi:10.1016/j.msec.2021.111967

Cited by 24 publications

(14 citation statements)

References 58 publications

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“…The addition of GO into PEEK supported the adhesion and spreading of bone MSCs [143]. Huang et al [144] described a NCP composed of PEEK, in which GO and HA were incorporated. After laser treatment, the composite had surface macropores with diameters from 200 µm to 600 µm, which improved cell adhesion and proliferation of constant cells and thus overall biocompatibility and utilization [144].…”

Section: Graphene-based Materialsmentioning

confidence: 99%

“…Huang et al [144] described a NCP composed of PEEK, in which GO and HA were incorporated. After laser treatment, the composite had surface macropores with diameters from 200 µm to 600 µm, which improved cell adhesion and proliferation of constant cells and thus overall biocompatibility and utilization [144]. Lopes et al [145] prepared HA-GO NCPs with the addition of 0.5 wt%, 1.0 wt%, and 1.5 wt% of GO.…”

Section: Graphene-based Materialsmentioning

confidence: 99%

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Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Jampílek

Plachá

2021

Pharmaceutics

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Since the worldwide incidence of bone disorders and cartilage damage has been increasing and traditional therapy has reached its limits, nanomaterials can provide a new strategy in the regeneration of bones and cartilage. The nanoscale modifies the properties of materials, and many of the recently prepared nanocomposites can be used in tissue engineering as scaffolds for the development of biomimetic materials involved in the repair and healing of damaged tissues and organs. In addition, some nanomaterials represent a noteworthy alternative for treatment and alleviating inflammation or infections caused by microbial pathogens. On the other hand, some nanomaterials induce inflammation processes, especially by the generation of reactive oxygen species. Therefore, it is necessary to know and understand their effects in living systems and use surface modifications to prevent these negative effects. This contribution is focused on nanostructured scaffolds, providing a closer structural support approximation to native tissue architecture for cells and regulating cell proliferation, differentiation, and migration, which results in cartilage and bone healing and regeneration.

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Section: Graphene-based Materialsmentioning

confidence: 99%

Section: Graphene-based Materialsmentioning

confidence: 99%

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Jampílek

Plachá

2021

Pharmaceutics

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“…with inorganic ceramic (hydroxyapatite, bioactive glass, etc.) attract great interest [1][2][3][4]. In previous studies, poly(lactide-coglycolide) (PLGA) was always chosen due to its good biocompatibility and biodegradability [1,2].…”

Section: Introductionmentioning

confidence: 99%

PLGA/BGP/Nef Porous Composite Restrains Osteoclasts by Inhibiting the NF-κB Pathway, Enhances IGF-1-mediated Osteogenic Differentiation and Promotes Bone Regeneration

et al. 2023

Preprint

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Background Novel bone substitutes are urgently needed in experimental research and clinical orthopaedic applications. There are many traditional Chinese medicines that have effects on bone repair. However, application of natural medicines in traditional Chinese medicine to bone tissue engineering and its mechanism were rarely reported. Results In this study, the drug loading and controlled release ability and osteogenic ability of bioactive glass particles (BGPs) and the osteogenic and osteoclastic ability of neferine were fused into PLGA-based bone tissue engineering materials for bone regeneration. BGPs were prepared by spray drying and calcination for loading of Nef. Particles loaded with drugs were then mixed with PLGA solution to prepare porous composites by the phase conversion method. Here we showed that Nef inhibited proliferation and enhanced ALP activity of MC-T3-E1 cells in a dose- and time‐dependent manner. And the composites containing Nef could also inhibit RANKL‐induced osteoclast formation (p < 0.05). Mechanistically, the PLGA/BGP/Nef composite downregulated the expression of NFATC1 by inhibiting the NF-κB pathway to restrain osteoclasts. In the other hands, PLGA/BGP/Nef composite was first demonstrated to effectively activate the IGF-1R/PI3K/AKT/mTOR pathway to enhance IGF-1-mediated osteogenic differentiation. The results of animal experiments show that the material can effectively promote the formation and maturation of new bone in the skull defect site. Conclusions The PLGA/BGP/Nef porous composite can restrain osteoclasts by inhibiting the NF-κB pathway, enhance IGF-1-mediated osteogenic differentiation and promotes bone regeneration, and has the potential for clinical application.

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“…Among these methods, the use of HA combined with the porosity has been considered a desirable technology for improving the osteointegration of PEEK-based devices ( Torstrick et al, 2018 ). Some investigators have reported that the incorporation of bioactive molecules and porosity to PEEK are effective approaches to improve the osteointegration of PEEK-based implants ( Chubrik et al, 2020 ; Conrad and Roeder, 2020 ; Swaminathan et al, 2020 ; Huang et al, 2021 ; Venkatraman et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Fabrication of In Situ Grown Hydroxyapatite Nanoparticles Modified Porous Polyetheretherketone Matrix Composites to Promote Osteointegration and Enhance Bone Repair

Wang

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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The repairment of critical-sized bone defects is a serious problem that stimulates the development of new biomaterials. In this study, nanohydroxyapatite (nHA)-doped porous polyetheretherketone (pPEEK) were successfully fabricated by the thermally induced phase separation method and hydrothermal treatment. Structural analysis was performed by X-ray diffraction. The water contact angles and scanning electron microscopy were measured to assess physical properties of surfaces. The mechanical strength of the composites is also determined. Microcomputed tomography is used to characterize the nHA content of the composites. The in vitro bioactivity of the composites with or without nHA was investigated by using murine pre-osteoblasts MC3T3-E1, and the results of cytotoxicity and cell proliferation assays revealed that the cytocompatibility of all specimens was good. Adherence assays were employed to examine the adhesion and morphology of cells on different materials. However, nHA-doped composites induced cell attachment and cell spreading more significantly. Osteogenic differentiation was investigated using alkaline phosphatase activity and alizarin red staining, and these in vitro results demonstrated that composites containing nHA particles enhanced osteoblast differentiation. Its effectiveness for promoting osteogenesis was also confirmed in an in vivo animal experiment using a tibial defective rat model. After 8 weeks of implantation, compared to the pure PEEK and pPEEK without nHA groups, the nHA-pPEEK group showed better osteogenic activity. The results indicate that the nHA-pPEEK composites are possibly a well-designed bone substitute for critical-sized bone defects by promoting bone regeneration and osteointegration successfully.

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Simultaneous engineering of nanofillers and patterned surface macropores of graphene/hydroxyapatite/polyetheretherketone ternary composites for potential bone implants

Cited by 24 publications

References 58 publications

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

PLGA/BGP/Nef Porous Composite Restrains Osteoclasts by Inhibiting the NF-κB Pathway, Enhances IGF-1-mediated Osteogenic Differentiation and Promotes Bone Regeneration

Fabrication of In Situ Grown Hydroxyapatite Nanoparticles Modified Porous Polyetheretherketone Matrix Composites to Promote Osteointegration and Enhance Bone Repair

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