Simultaneous electrochemical detection of multiple tumor markers using metal ions tagged immunocolloidal gold

Single electrode biosensor for simultaneous determination of interferon gamma and lysozyme

“…Electrochemical analysis has the advantages of high sensitivity, compatibility for miniaturization, easy operation and low cost, making it an attractive candidate for two biomarkers testing systems (Wang, 2002;Lee and Hsing, 2006). However, the vast majority of reports are concerned with the detection of only single analyte (for Lys, Jing et al, 2010;Subramanian et al, 2013;Lian et al, 2014;Erdema et al, 2014;for IFN-γ, Chang et al, 2012;Chen et al, 2014;Zhang et al, 2012;Zhao et al, 2012;Jiang et al, Xu et al (2014) proposed a sandwitch-format electrochemical immunosensor for the simultaneous detection of two tumor markers (carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP)). Qi et al (2012) developed an electrochemical immunoassay array for the simultaneous detection of CEA and AFP by incorporating electrochemically addressing immobilization and one signal antibody strategy.…”

Design of immunoprobes for electrochemical multiplexed tumor marker detection

“…AuNPs have excellent electrical conductivity and biocompatibility, which can be used as immunoprobe carrier for simultaneous multianalyte detection [55]. The multiplexed immunoassay exhibited good sensitivity and selectivity for the simultaneous determination of CEA and AFP with linear ranges of 0.01-50 ng ml À1 and detection limits 4.6 and 3.1 pg ml À1 , respectively.…”

“…HRP-hollow Pt nanoparticles CEA: 50.0 AFP: 80.0 CEA: 0.5-50 AFP: 0.3-45 [52] HRP and GOD/graphene-Pt CEA: 1.64 AFP: 1.33 CEA: 0.01-100 AFP: 0.01-100 [53] AuNPs CEA: 4.6 AFP: 3.1 CEA: 0.01-50 AFP: 0.01-50 [55] Hollow gold nanosphere CEA: 1.0 AFP: 1.0 CEA: 0.01-80 AFP: 0.01-200 [56] Platinum porous nanoparticles CEA: 2.0 AFP: 50.0 CEA: 0.05-200 AFP: 0.05-200 [57] PtPd nanocage CEA: 1.4 AFP: 1.0 CEA: 0.05-200 AFP: 0.03-100 [58] Redox probe modified rGO CEA: 3.0 AFP: 2.0 CEA: 0.01-100 AFP: 0.01-100 [61] Cyclodextrin-modified graphene CEA: 0.5 AFP: 0.8 CEA: 0.003-40 AFP: 0.001-60 [62] Onion-like mesoporous graphene PSA: 3.4 fPSA: 6.7 PSA: 0.02-10 fPSA: 0.01-50 [63] AuNPs-carbon nanotubes AFP: 8. prepared by large number of HRP-and GOD-loaded graphene-platinum nanocomposites as immunoprobe carrier [53]. The sensitivity of immunosensor was greatly amplified because of excellent catalytic performance to glucose and H 2 O 2 .…”

Simultaneous quantitative detection of multiple tumor markers with a rapid and sensitive multicolor quantum dots based immunochromatographic test strip

“…Various approaches have been proposed to perform simultaneous detection of multiple tumor markers in the past few years. For example, electrochemical immunoassay (Wilson, 2007;Xu et al, 2014), label-free methods (Lin et al, 2011;Kong et al, 2013), dielectrophoresi assays (Lee et al, 2010;Ramón-Azcón et al, 2011), chemiluminescence alalysis (Zong et al, 2012;Guo et al, 2013), biobarcoded Scanometric assay (Stoeva et al, 2006), spectrometry (Chon et al, 2011;Giesen et al, 2011), fluorescence detection (Tian et al, 2012;Akinfieva et al, 2013), photonic suspension array (Zhao et al, 2009). But all these techniques are not suitable for point-of-care testing because they require large and sophisticated instruments, complex operations, long analysis time and highly skilled personnel to manipulate.…”