Simultaneous Electrochemical Analysis of Hydrophilic and Lipophilic Antioxidants in Bicontinuous Microemulsion

Kuraya, Eisuke; Nagatomo, Shota; Sakata, Kouhei; Kato, Dai; Niwa, Osamu; Nishimi, Taisei; Kunitake, Masashi

doi:10.1021/ac5044576

Cited by 27 publications

(35 citation statements)

References 31 publications

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“…DPPH and ABTS + radical scavenging activities are more widely employed for screening in vitro antioxidant activities. One peculiarity of these two assays is that they are applicable to both lipophilic and hydrophilic compounds . In addition, the principle of reducing power assay is to evaluate the ability of antioxidant to donate electrons .…”

Section: Resultsmentioning

confidence: 74%

Protective effects of antioxidant egg‐chalaza hydrolysates against chronic alcohol consumption‐induced liver steatosis in mice

Yang

Lin

et al. 2018

J Sci Food Agric

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BACKGROUND: Reactive oxygen species (ROS) overproduction is highly related to some human chronic diseases. There are approximately 400 metric tons of chalazae produced yearly after the processing of the liquid-egg production, which are disposed of as waste. The objectives of this study were to look for the optimal production condition of antioxidant crude chalaza hydrolysates and evaluate the in vivo antioxidant capacity via a chronic alcohol consumption mouse model. RESULTS: Antioxidant crude chalaza hydrolysates (CCH-As) could be produced by proteaseA at 1:100 ratio (w/w) and 0.5 h hydrolytic period. After our analyses, CCH-As were rich in leucine, arginine, phenylalanine, valine, lysine and antioxidant dipeptides (anserine and carnosine), and the major molecular masses were lower than 15 kDa. Regarding protective effects of CCH-As against oxidative damage in alcoholic-liquid-diet-fed mice, alcohol-fed mice had lower (P < 0.05) liver antioxidant capacities, and higher (P < 0.05) liver lipid contents, serum lipid/liver damage indices and IL-1 /IL-6 values. CCH-A supplementation reversed (P < 0.05) liver antioxidant capacities and reduced (P < 0.05) serum/liver lipids in alcohol-fed mice, which may result from increased (P < 0.05) fecal lipid output, upregulated (P < 0.05) fatty acid -oxidation and downregulated (P < 0.05) lipogenesis in the liver. CONCLUSION: Taken together, this CCH-A should benefit the liquid-egg industry, while also offering consumers a choice of healthy ingredients from animal sources.Keywords: alcoholic fatty liver; amino acid profile; in vitro and in vivo antioxidant effects; lipid metabolism; protease-A-digested crude chalaza hydrolysate Industry and 0.25 mL potassium ferricyanide (1%) (Sigma Co.), and the mixture was incubated at 50 ∘ C for 15 min. 0.25 mL trichloroacetic acid solution (10%) (Sigma Co.) was added to the mixture, which was then centrifuged at 900 × g for 10 min at room temperature. Then J Sci Food Agric 2019; 99: 2300-2310In vivo hepatoprotective effects of CCH-As Effects of CCH-As on body weight, size of organs and abdominal fat pad, serum biochemical values, liver damage status, and hepatic and fecal lipids in chronic alcohol-fed mice With regard to an in vivo antioxidant effect of our manufactured CCH-As, chronic alcoholic liquid-diet-fed mice were applied as J Sci Food Agric 2019; 99: 2300-2310

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Section: Resultsmentioning

confidence: 74%

Protective effects of antioxidant egg‐chalaza hydrolysates against chronic alcohol consumption‐induced liver steatosis in mice

Yang

Lin

et al. 2018

J Sci Food Agric

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“…One of the possible strategies is to use a microemulsion, which is a thermodynamically stable system that consists of organic and aqueous phases, plus surfactants. In a microemulsion, the electrochemical contact between the electrode and both micro-aqueous and organic phases can be simultaneously achieved by controlling the hydrophilicity and lipophilicity of the electrode surface [6][7][8][9][10]. So far, Rusling and co-workers have investigated the direct electrochemical reduction of arenes such as naphthalene and biphenyl at a non-catalyzed carbon electrode in neutral microemulsion media where the substrates were diluted with an organic solvent such as hexane [11,12].…”

Section: Introductionmentioning

confidence: 99%

Direct electrochemical hydrogenation of toluene at Pt electrodes in a microemulsion electrolyte solution

Wakisaka

Kunitake

2016

Electrochemistry Communications

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In the present study, we demonstrated a new concept for the direct electrochemical hydrogenation of toluene using an acidic microemulsion electrolyte with active Pt electrodes to pave the way for efficient methylcyclohexane mass production. We have achieved a Faradaic efficiency of 80% for the toluene/methylcyclohexane conversion at a Pt black electrode, under galvanostatic conditions and in a one-compartment cell. The reaction rate and selectivity of the toluene reduction were found to depend strongly on the surface structure of the Pt electrodes.

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“…4 B while maintaining sufficient electrochemical activity for FcPMB. (vitamin E) in olive oil samples by using a bicontinuous microemulsion (BME) solution and can selectively detect lipophilic vitamin E 60,61 with no interference of L-ascorbic acid which is a well-known hydrophilic antioxidant.…”

Section: Analytical Sciencesmentioning

confidence: 99%

Hybrid Carbon Film Electrodes for Electroanalysis

et al. 2020

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Carbon materials have been widely used for electrochemical analysis including carbon nanotubes, graphene, and boron-doped diamond electrodes in addition to conventional carbon electrodes such as those made of glassy carbon and graphite. Of the carbon-based electrodes, carbon film has advantages because it can be fabricated reproducibly and micro-or nanofabricated into electrodes with a wide range of shapes and sizes. Here, we report two categories of hybrid-type carbon film electrodes for mainly electroanalytical applications. The first category consists of carbon films doped or surface terminated with other atoms such as nitrogen, oxygen and fluorine, which can control surface hydrophilicity and lipophilicity or electrocatalytic performance, and that are used to detect various electroactive biochemicals. The second category comprises metal nanoparticle embedded in carbon film electrodes fabricated by co-sputtering, which exhibits high electrocatalytic activity for environmental and biological samples including toxic heavy metal ions and clinical sugar markers, which are difficult to detect at pure carbon-based electrodes.

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Simultaneous Electrochemical Analysis of Hydrophilic and Lipophilic Antioxidants in Bicontinuous Microemulsion

Cited by 27 publications

References 31 publications

Protective effects of antioxidant egg‐chalaza hydrolysates against chronic alcohol consumption‐induced liver steatosis in mice

Protective effects of antioxidant egg‐chalaza hydrolysates against chronic alcohol consumption‐induced liver steatosis in mice

Direct electrochemical hydrogenation of toluene at Pt electrodes in a microemulsion electrolyte solution

Hybrid Carbon Film Electrodes for Electroanalysis

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