“…Ph.) and its variations like, HPLC-UV-detector [19,21,22], HPLC-with electrochemical detector [23][24], HPLC-isotope dilution thermospray mass spectrometry [25], and also methods like thin layer chromatography [7,19,26], liquid chromatography with mass spectral detection [21,27,28], capillary zone electrophoresis-mass spectrometry [20], capillary electrophoresis-mass spectrometry [11,29], radio-immune analysis [30,31] etc. Many of these methods are characterized by highly labor intensive, the high cost of equipment's, long duration of analysis time, as well as usually highly toxic chemicals, are required for an analysis procedure.…”