Simultaneous determination of vinblastine and its monomeric precursors vindoline and catharanthine in Catharanthus roseus by capillary electrophoresis–mass spectrometry

Abstract

Catharanthus roseus is an important dicotyledonous medicinal plant that contains various anticancer components, such as vinblastine (VLB) and its monomeric precursors (vindoline and catharanthine). A capillary electrophoresis-mass spectrometry (CE-MS) approach for the simultaneous determination of three components was developed in this work. Baseline separation for three components was achieved by using a running buffer consisting of 20 mM ammonium acetate and 1.5% acetic acid in <20 min. Quantification of three components was assigned in positive-ion mode at a protonated molecular ion [M+H](+). The CE-MS method was validated for linearity, sensitivity, accuracy and precision, and then used to determine the content of the above components. The detection limits of VLB, catharanthine and vindoline are 0.8, 0.1 and 0.1 μg/mL, respectively. The precision was not more than 4.54% and the mean recovery of the analytes was 95.04-97.04%. The CE-MS method was successfully applied to determine VLB and its monomeric precursors in real sample C. roseus.

Antineoplastic drugs and their analysis: a state of the art review

“…The analysis of vinca alkaloids has been carried out on plant extracts, [398][399][400][401][402][403] pharmaceutical formulations, 316,404 biological samples 358,359,[405][406][407][408][409][410][411][412][413][414][415][416][417][418][419][420]421 and environmental samples. 18,19,134,307,422 Vinca alcaloid extraction from plants was generally performed by ultrasound in acid media followed by LLE.…”

“…18,19,134,307,422 Vinca alcaloid extraction from plants was generally performed by ultrasound in acid media followed by LLE. 45,[398][399][400][401] CE methods are scarcely reported for the determination of vinca alkaloids. A CE-UV method in non-aqueous conditions (NACE) allowed the separation of ten different vinca alkaloids in less than 10 min.…”

“…A CE-UV method in non-aqueous conditions (NACE) allowed the separation of ten different vinca alkaloids in less than 10 min. 404 Extracts from Catharanthus roseus containing vinblastine were also successfully analysed by CE-MS. 398 Finally, the analysis of vinorelbine in urine samples was achieved by CE coupled to an electrochemiluminescence detection (ECL) with LOD in the order of 7 ng mL −1 . …”

Validated Spectrophotometric Method for the Estimation of Vincristine and Vinblastine

“…C. roseus has been used in traditional medicine in tropical and subtropical countries for the treatment of diabetes, hypertension, bleeding, scurvy, toothaches, and others from ancient time. [7] At the moment in vivo and in-vitro studies have demonstrated hypoglycemic, antihypertensive, anti-bacterial, anti-malarial, antiarrhythmic and other properties of C. roseus [9][10][11][12][13][14].…”

“…Ph.) and its variations like, HPLC-UV-detector [19,21,22], HPLC-with electrochemical detector [23][24], HPLC-isotope dilution thermospray mass spectrometry [25], and also methods like thin layer chromatography [7,19,26], liquid chromatography with mass spectral detection [21,27,28], capillary zone electrophoresis-mass spectrometry [20], capillary electrophoresis-mass spectrometry [11,29], radio-immune analysis [30,31] etc. Many of these methods are characterized by highly labor intensive, the high cost of equipment's, long duration of analysis time, as well as usually highly toxic chemicals, are required for an analysis procedure.…”