Simultaneous Determination of Tropane Alkaloids in Different Fractions of Herba belladonnae Collected in Various Seasons by HPLC Method

Abstract

An HPLC method was developed for the simultaneous determination of hyoscyamine, scopolamine and anisodamine to investigate medical parts of Herba belladonnae in different harvest time. The solid phase was Shim-pack VP-ODS (4.6 mm × 250 mm, 5 µm), with isocratic elution using 0.1 % phosphoric acid solution (including 0.25 % SDS)-acetonitrile (60:40) as the mobile phase. The flow rate was 1 mL/min and the wavelength was 210 nm. The results of methodology all fit the analytical requests and the recovery were 97.3-103.0 %. Quantitative analysis of the three compounds showed that the contents of hyoscyamine in leaves and flowers were the highest, followed by fruits and roots and the third was stem. The contents of scopolamine in flowers and fruits were the highest. The contents of anisodamine in fruits were the highest. The relative contents of hyoscyamine showed an increasing trend during the growth period, while scopolamine and anisodamine showed an opposite trend, which would reach a balance finally. The method was simple, quick and reliable and could provide a scientific and technical platform for setting up a quality control standard.

Characterization of tropane and cinnamamide alkaloids from Scopolia tangutica by high‐performance liquid chromatography with quadrupole time‐of‐flight tandem mass spectrometry

“…HPLC–MS/MS was potential in structural identification from TCMs . HPLC exhibited an excellent separation resolution and selectivity in analysis of complex samples . MS/MS was powerful in rapid structural characterization by providing precise molecular weights and rich fragment ions.…”

“…It suggests that finding more active compounds from traditional Chinese medicines (TCMs) is of great importance.HPLC-MS/MS was potential in structural identification from TCMs [9][10][11]. HPLC exhibited an excellent separation resolution and selectivity in analysis of complex samples [12][13][14][15]. MS/MS was powerful in rapid structural characterization by providing precise molecular weights and rich fragment ions.…”

Discovery of new muscarinic acetylcholine receptor antagonists from Scopolia tangutica

“…First, traditional approaches such as solvent extraction followed by thin-layer chromatography1415 or one-dimensional (1D) high performance liquid chromatography-mass spectrometry (HPLC-MS)161718 often result in isolation and identification of small sets of compounds because of the chemical diversity of many medicinal plants. Second, our recent non-aqueous solid phase extraction (SPE)19 and 2D-HPLC methods20 have resulted in the identification of four hydroxycinnamic acid amides from S. tangutica for the first time21.…”