Characterization of tropane and cinnamamide alkaloids from Scopolia tangutica by high‐performance liquid chromatography with quadrupole time‐of‐flight tandem mass spectrometry

Abstract

Scopolia tangutica is a traditional Chinese medicine used for antispasmodic, anesthesia, analgesia, and sedation. Its medicinal activity is associated to alkaloid constituents, including tropane and cinnamamide types. Low content of alkaloids in plant makes them difficult to be isolated and identified. The present work developed an effective method to quickly characterize alkaloids from Scopolia tangutica by high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry. Thirteen reference compounds were studied for their fragmentation pathways, including five tropane alkaloids and eight cinnamamide ones. Alkaloid constituent was analyzed by an optimized high‐performance liquid chromatography method and mass spectrometry analysis to achieve systematic characterization of alkaloids from Scopolia tangutica. As a result, 53 compounds were identified, including 21 tropane alkaloids (eight new ones), 18 caffeoyl ones (ten new ones) and 14 dicaffeoyl ones (seven new ones). It was important to provide rich information in phytochemical study and structure‐guided isolation of important compounds from this plant.

Anisotanols A—D, Four Norsesquiterpenoids with an Unprecedented Sesquiterpenoid Skeleton from Anisodus tanguticus‡

“…In recent years, Liang and coauthors have achieved several good achievements in bioactive alkaloids of A. tanguticus . [ 14‐17 ] However, no sesquiterpenoids have been isolated from A. tanguticus so far. In this paper, a phytochemical investigation of A. tanguticus resulted in the isolation and characterization of four novel sesquiterpenoids (anisotanols A—D), which represent a new class of sesquiterpenoids featuring a congested tricyclic 6/3/5 ring system (Figure 1).…”

Interpol review of controlled substances 2016–2019

“…Scopolia tangutica: 2019 LC-MS/MS method to characterize alkaloids from Scopolia tangutica [ 850 ]; SPE enrichment method to prevent co-elution of alkaloids from Scopolia tangutica (also applied to other plants) [ 851 ].…”

Alkaloid purification using rosin‐based polymer‐bonded silica stationary phase in HPLC

“…To date, the common extraction method for alkaloids is LLE including base, acid, and alcohol extraction. Unfortunately, the inefficient extraction yield of alkaloids from crude plants limited their application . In addition, waste liquid from alkaline or acid solution extraction resulted in environmental and after‐treatment issues.…”

“…To date, the common extraction method for alkaloids is LLE including base, acid, and alcohol extraction. Unfortunately, the inefficient extraction yield of alkaloids from crude plants limited their application [1][2][3][4]. In addition, waste liquid from alkaline or acid solution extraction resulted in environmental Article Related Abbreviations: AIBN, azodiisobutyronitrile; CAF, caffeine; CPT, (s)-(+)-camptothecin; EGMRA, ethylene glycol maleic rosinate acrylate; EVO, evodiamine; HAc, acetic acid; HCPT, (s)-10-hydroxycamptothecin; MAA, methacrylic acid; MeOH, methanol; MPTMS, 3-(methacryloyloxy)-propyltrimethoxysilane; RPS, rosin-based polymer-bonded silica particle; RPS-column, rosin-based polymer bonded silica HPLC column; Rs, resolution; RUT, rutecarpine; TEO, theophylline.…”