Simultaneous determination of total hypericin and hyperforin in St. John's wort extracts by HPLC with electrochemical detection

Rückert, Ulla; Likussar, W.; Michelitsch, Astrid

doi:10.1002/pca.969

Cited by 10 publications

(7 citation statements)

References 17 publications

(18 reference statements)

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“…In the same setting, the limit of detection for hyperforin was 0.05 ng . An improved method with amperometric detection allowed the simultaneous determination of total hypericin (protopseudohypericin, pseudohypericin, protohypericin and hypericin) and hyperforin …”

Section: Electroanalytical Methodsmentioning

confidence: 99%

“…[97] An improved method with amperometric detection allowed the simultaneous determination of total hypericin (protopseudohypericin, pseudohypericin, protohypericin and hypericin) and hyperforin. [98] A capillary zone electrophoretic method was established for the determination of rutin from H. perforatum extracts. The analysis was performed using a fused-silica capillary, the background electrolyte consisted of 10% ethanol and 20 mM borate buffer (pH = 8.0).…”

Section: Electroanalytical Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2017

Journal of Pharmacy and Pharmacology

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Objectives The most widely applied qualitative and quantitative analytical methods in the quality control of Hypericum perforatum extracts will be reviewed, including routine analytical tools and most modern approaches. Key findings Biologically active components of H. perforatum are chemically diverse; therefore, different chromatographic and detection methods are required for the comprehensive analysis of St. John's wort extracts. Naphthodianthrones, phloroglucinols and flavonoids are the most widely analysed metabolites of this plant. For routine quality control, detection of major compounds belonging to these groups seems to be sufficient; however, closer characterization requires the detection of minor compounds as well. Conclusions TLC and HPTLC are basic methods in the routine analysis, whereas HPLC‐DAD is the most widely applied method for quantitative analysis due to its versatility. LC‐MS is gaining importance in pharmacokinetic studies due to its sensitivity. Modern approaches, such as DNA barcoding, NIRS and NMR metabolomics, may offer new possibilities for the more detailed characterization of secondary metabolite profile of H. perforatum extracts.

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Section: Electroanalytical Methodsmentioning

confidence: 99%

Section: Electroanalytical Methodsmentioning

confidence: 99%

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2017

Journal of Pharmacy and Pharmacology

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“…ECD was found to be more sensitive than UV for the normal-phase HPLC profiling of diarylheptanoids in Alpinia officinarum (lesser galangal) [49]. Similarly, the simultaneous determination of hyperforin and total hypericin in Hypericum perforatum was achieved after the photoconversion of the protoforms of hypericins in extracts and tablets [50]. The antioxidant potential of polyphenols can be estimated by HPLC with coulometric array detection because they are reducing agents, and their electrochemical responses result from the donation of electrons.…”

Section: Hplc-ecd (Electrochemical Detection)mentioning

confidence: 99%

HPLC in Natural Product Analysis: The Detection Issue

2009

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High-performance liquid chromatography (HPLC) is a very powerful and versatile chromatographic technique for the separation of natural products (NPs) in complex matrices, such as crude extracts for selective detection and quantification or general profiling. The method is widespread and has been adapted to the analysis of a broad range of NPs generally without the need for complex sample preparation. The choice of the appropriate detection method in HPLC is crucial because of the diversity of NPs and the fact that there is no single technique for their efficient detection. In this review both qualitative and quantitative applications of HPLC with UV, DAD, FD, ECD, RID, FID, CL, ESLD, CAD, MS, MS-MS, and NMR are covered to provide a general, rather than an exhaustive, overview. The potential and limitations as well as some new trends in HPLC hyphenation are discusse

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“…Hypericin and hyperforin (6, 7, Fig. 1) have been found to possess cancer chemopreventive activities (Bilia et al 2002;Butterweck 2003;Ruckert et al 2007).…”

Section: Hypericum Perforatum Linnmentioning

confidence: 98%

Anticancer potential of Himalayan plants

et al. 2010

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Plants used in traditional medicine have stood up to the test of time and contributed many novel compounds for preventive and curative medicine to modern science. India is sitting on a gold mine of well recorded and traditionally well practiced knowledge of herbal medicine. Specially, plants growing at high altitude in Himalayan pastures are time-honored sources of health and general well being of local inhabitants. As of today, Himalayan plants are a major contributor to the herbal pharmaceutical industry both of India and other countries. Plants growing at higher altitudes are subjected to an assault of diverse testing situations including higher doses of mutagenic UV-radiation, physiological drought, desiccation and strong winds. Plants interact with stressful environments by physiological adaptation and altering the biochemical profile of plant tissues and producing a spectrum of secondary metabolites. Secondary metabolites are of special interest to scientists because of their unique pharmacophores and medicinal properties. Secondary metabolites like polyphenols, terpenes and alkaloids have been reported to possess antimutagenic and anticancer properties in many studies. The fundamental aspiration of the current review is to divulge the antimutagenic/anticancer potential of five alpine plants used as food or medicine by the populations living at high altitudes.

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Simultaneous determination of total hypericin and hyperforin in St. John's wort extracts by HPLC with electrochemical detection

Cited by 10 publications

References 17 publications

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Quality control of Hypericum perforatum L. analytical challenges and recent progress

HPLC in Natural Product Analysis: The Detection Issue

Anticancer potential of Himalayan plants

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