Simultaneous determination of thyroxine and triiodothyronine in pharmaceutical formulations using capillary electrophoresis with amperometric detection

Sun, Yan; Zhao, Xiaoyin; Li, Ping; Shi, Guoyue; Zhou, Tianshu

doi:10.1002/jssc.201000077

Cited by 15 publications

(4 citation statements)

References 31 publications

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“…CE also offers the possibility that a single set of separation conditions can be applied for a wide range of analyses, giving efficiency savings . Recently, CE techniques have been applied to the simultaneous determination of coformulated drugs in dosage forms .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of aliskiren hemifumarate, amlodipine besylate, and hydrochlorothiazide in their triple mixture dosage form by capillary zone electrophoresis

et al. 2014

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A novel, specific, reliable, and accurate capillary zone electrophoretic method was developed and validated for the simultaneous determination of aliskiren hemifumarate, amlodipine besylate, and hydrochlorothiazide in their triple mixture dosage form. Separation was carried out in a fused-silica capillary (57.0 cm total length and 50.0 cm effective length, 75.6 μm internal diameter) by applying a potential of 17 kV and a running buffer consisting of 40 mM phosphate buffer at pH 6.0 with UV detection at 245 nm. The method was suitably validated with respect to specificity, linearity, LOD, and LOQ, accuracy, precision, and robustness. The method showed good linearity in the ranges 1-10, 2.5-25, and 30-300 μg/mL with LODs of 0.11, 0.33, and 5.83 μg/mL for amlodipine besylate, hydrochlorothiazide, and aliskiren hemifumarate, respectively. The proposed method was successfully applied for the analysis of the studied drugs in their coformulated tablets. The results of the proposed method were statistically compared with those obtained by the RP-HPLC reference method revealing no significant differences in the performance of the methods regarding accuracy and precision.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of aliskiren hemifumarate, amlodipine besylate, and hydrochlorothiazide in their triple mixture dosage form by capillary zone electrophoresis

et al. 2014

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“…The CE‐ED system was constructed in the laboratory and was similar to that described previously . Electrophoresis in the capillary was driven by a ±30 kV high‐voltage supplier (Shanghai Institute of Applied Physics, China).…”

Section: Methodsmentioning

confidence: 99%

Stacking and simultaneous determination of estrogens in water samples byCEwith electrochemical detection

Zhao

Wang

et al. 2012

J of Separation Science

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A rapid and sensitive method based on transient ITP and field enhancement in CE with electrochemical detection at copper disk electrode was developed for the simultaneous separation and determination of three estrogens: estrone, 17β-estradiol, and estriol. The effects of several important factors that influence the separation and detection were investigated. Under the optimum conditions, the estrogens could be separated in 0.06 mol/L sodium hydroxide solution within 14 min. With transient ITP by addition of 0.5% NaCl, a good linear response was obtained for three estrogens from 0.2 to 10 μmol/L, with correlation coefficients higher than 0.9993. The detection limits were 8.9 × 10(-8) , 6.7 × 10(-8) , and 1.1 × 10(-7) mol/L (S/N = 3) for estriol, 17β-estradiol, and estrone, respectively. This method was successfully employed to analyze different water samples from waterworks, tap water, fishpond, and river samples with recoveries in the range of 90.8-108.9%, and RSDs < 4.69%. The satisfied results demonstrated that this method was of convenient preparation, high sensitivity, and good repeatability, which could be applied to the rapid determination of environmental water samples.

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“…Due to its behavior under these conditions it is difficult to find an accurate and reliable analytical method for the drug determination in commercial tablets, especially considering that typical treatment doses are in small concentrations (in order of micrograms) . Methods for determination of NaLVT are mainly based on high‐performance liquid‐chromatography (HPLC) , fluorescence , chemiluminescence , capillary electrophoresis , mass spectrometry , immunoassay methods or voltammetry .…”

Section: Introductionmentioning

confidence: 99%

Batch‐injection Amperometric Analysis on Screen‐printed Electrodes: Analytical System for High‐throughput Determination of Pharmaceutical Molecules

et al. 2018

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This work presents a single analytical system able to perform high‐throughput determinations of different pharmaceutical molecules on screen‐printed electrodes (SPEs) assembled on a batch‐injection analysis (BIA) cell. Two types of SPEs, both containing a carbon conductive ink as working electrode, were selected for the determination of levamisole (LVM) in aqueous and sodium levothyroxine (NaLVT) in hydroethanolic media. The main analytical characteristics of the proposed system for both examples are high precision (RSD <3.8 %, n=10), low detection limits (submicromolar range), and high sample‐throughput (>150 h−1) using a single SPE, demonstrating the extended lifetime of such sensors, which are adequate for routine pharmaceutical analysis. The proposed analytical system requires battery‐powered portable devices, including potentiostat and reader, electronic micropipette, BIA cell and SPEs, and can be applied for a wide range of pharmaceutical molecules. In case of analyte adsorption on electrode surface, fast electrode cleaning can be supplied by external stirring easily adapted to the cell, which is demonstrated in this work for NaLVT determination.

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Simultaneous determination of thyroxine and triiodothyronine in pharmaceutical formulations using capillary electrophoresis with amperometric detection

Cited by 15 publications

References 31 publications

Simultaneous determination of aliskiren hemifumarate, amlodipine besylate, and hydrochlorothiazide in their triple mixture dosage form by capillary zone electrophoresis

Simultaneous determination of aliskiren hemifumarate, amlodipine besylate, and hydrochlorothiazide in their triple mixture dosage form by capillary zone electrophoresis

Stacking and simultaneous determination of estrogens in water samples byCEwith electrochemical detection

Batch‐injection Amperometric Analysis on Screen‐printed Electrodes: Analytical System for High‐throughput Determination of Pharmaceutical Molecules

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