Simultaneous determination of the antiretroviral agents: amprenavir, lopinavir, ritonavir, saquinavir and efavirenz in human peripheral blood mononuclear cells by high-performance liquid chromatography–mass spectrometry

A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study

“…Several LC-MS/MS methods described in the literature were developed for simultaneous quantification of various anti-HIV agents with a long analytical time. 16,24,26,27,29,43 This method for quantitation of efavirenz in human plasma show an analytical time of 2.0 min and was successfully applied in a bioequivalence study. The use of mass spectrometric detection in MRM mode is less demanding on chromatographic separation between analytes and early-eluting interferents due to improved selectivity and this feature can be explored to increase sample throughput by reducing sample analysis time, provided adequate selectivity without ion suppression is achieved.…”

“…This is because of its long half-life, which means effective concentrations are maintained for long periods. 3 Several methods are described in literature for the quantification of efavirenz in biological fluids by high performance liquid chromatography with ultraviolet detection (HPLC-UV), [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] HPLC with fluorescence detector, 23 liquid chromatography with mass spectrometry detection (LC-MS), 24 liquid chromatography with mass spectrometry in tandem detection(LC-MS/MS) [25][26][27][28][29] and matrix assisted laser desorption/ionization with time-of-flight in tandem detection (MALDI-TOF/ TOF). 30 When sample throughput is an important issue, such as in pharmacokinetic applications, the development of rugged methods with short analysis times becomes an important consideration.…”

A simple, rapid, economical, and practical method for the determination of efavirenz in plasma of Chinese AIDS patients by reverse phase high-performance liquid chromatography with ultraviolet detector

“…The method established in this paper, by RP-HPLC with UV detector, has a lower requirement for equipments compared to reported methods (17)(18)(19)(20)(21)(22)(23)(24)(25). In our method, diazepam was used as internal standard because of its cheapness, accessibility, and well-separated with efavirenz.…”

“…Up to now, several methods for the determination of efavirenz in plasma using LC-MS (17,18), LC-MS-MS (19,20), MALDI-TOF/IOF (21), HPLC-PAD (22)(23)(24)(25), etc., have been published. These methods may not be widely used in underdeveloped countries because of the inaccessibility and high costs of laboratory equipments.…”

Determination of salivary efavirenz by liquid chromatography coupled with tandem mass spectrometry

“…Several analytical methods have been published for the analysis of EFV in plasma, either alone or in combination with other drugs, using HPLC-UV [14][15][16][17] and LC-MS/MS [18][19][20][21][22][23] techniques. Determination of EFV levels in human hair [7] and reports of determining nevirapine [8,10] and indinavir [24] from saliva have been published, but this study is the first validated LC-MS/MS method for the assay of EFV in saliva to be reported.…”