Simultaneous Determination of Strychnine, Brucine, Strychnine N-Oxide and Brucine N-Oxide in Crude and Fermented Nux Vomica by CZE with Ephedrine Hydrochloride as an Internal Standard

Yang, Guangming; Pan, Yang; Liu, Liangjing; Jiang, Yaping

doi:10.1007/s10337-011-1912-y

Cited by 8 publications

(4 citation statements)

References 15 publications

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“…This analytical method with low intra‐ and interday variations had detection limits of 0.83 ppm for strychnine, 0.96 ppm for brucine, 2.10 ppm for strychnine N ‐oxide, and 2.50 ppm for brucine N ‐oxide. Strychnine N ‐oxide and brucine N ‐oxide were found only in fermented nux vomica, which indicates that strychnine and brucine in crude nux vomica might be partly transferred to their N ‐oxides .…”

Section: Analyte Classes Important In Bioprocess Analysismentioning

confidence: 95%

Capillary electrophoresis for monitoring bioprocesses

2013

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Chemical characterization and monitoring of fermentation broths and cell culture media provide significant information on the changes occurring within these complex and dynamic systems. Analytical methods based on CE in capillaries and microchips are attractive for integration in instrumental tools to obtain this critical data, improving the understanding and control of bioprocesses. In this review, the use of CE for chemical characterization and monitoring fermentations is discussed, organized by analyte class, including organic acids, pharmaceuticals, proteins, sugars, amino acids, and metabolites published between 1992 and October 2012. A section is dedicated to the roles CE plays throughout the wine making process, where applications range from characterization and increase in fundamental understanding of the fermentation to forensic applications, verifying the authenticity of the wine.

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Section: Analyte Classes Important In Bioprocess Analysismentioning

confidence: 95%

Capillary electrophoresis for monitoring bioprocesses

2013

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“…In the next work, CE–ELCL was applied to monitor the concentration of toxic diester‐diterpenoid alkaloids in Radix Aconiti . Lastly, CZE–UV was employed for the simultaneous determination of strychnine, brucine, strychnine N ‐oxide, and brucine N ‐oxide in crude and fermented nux vomica . It is noted that for human consumption, crude nux vomica containing toxic strychnine and brucine must be processed by fermentation to their less toxic N ‐oxide derivatives.…”

Section: Applicationsmentioning

confidence: 99%

Capillary electrophoresis of natural products: 2011–2012

et al. 2014

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Bioactive natural products are major sources of lead compounds for drug discovery and pharmaceutical development, therefore, innovative and current separation and characterization techniques are important for these compounds. Here, CE methods applied for the analysis of natural products published during 2011-2012 are reviewed. This is an updated version of an earlier review paper in this journal, which highlighted developments during 2006-2010. The major method developments over the review period centered on derivatization, chiral analysis, modes of detection, stacking or on-line sample concentration, and sample preparation (predominantly using extraction methods). The samples analyzed were herbal products, foods, soil, and biological samples. Developments also occurred in the areas of quality control, toxicology assessment, and enzyme-inhibitor screening. A table that summarizes the areas, source of natural product, nature of the bioactive analyte, CE conditions, LODs, and corresponding reference is provided. A short description on the theory of CE and insights on future activities of CE on natural products are also presented.

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“…Semen Strychni is a traditional Chinese herb, mainly used to treat rheumatoid arthritis, nervous disease, and traumatic pains and to promote blood circulation and remove blood stasis [ 1 – 3 ]. Total alkaloid from Semen Strychni (TAS) is a concentrated bioactive extract of Semen Strychni , which mainly consists of strychnine and brucine [ 4 , 5 ]. Strychnine and brucine possess analgesic, anti-inflammatory, and antitumor effects as well as toxicity [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Renoprotective Effects of Total Glucosides from Paeony against Nephrotoxicity Induced by Total Alkaloids from Semen Strychni

Zhang

Cheng

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Semen Strychni have been shown to have therapeutic effect in improving blood circulation, relieving rheumatic pain, and treating cancer. However, Semen Strychni could cause severe nephrotoxicity. The present study was designed to evaluate whether treatment with total glucosides from paeony (TGP) has renoprotective effect against nephrotoxicity induced by total alkaloids from Semen Strychni (TAS). The levels of blood urea nitrogen (BUN) and creatinine (Cr) were determined and histopathological changes were also examined to evaluate renal injury. Moreover, a HPLC-MS method was developed and validated to investigate the comparative toxicokinetics of strychnine and brucine in rats plasma after oral administration of TAS and pretreatment with TGP. Results demonstrated that the levels of BUN and Cr were significantly increased (p < 0.05) in TAS group, together with tubule epithelium cloudy swelling, degeneration, and glomerular atrophy in rats' kidneys. The TAS-induced kidney damage was alleviated after pretreatment with TGP. Besides, Tmax of strychnine and brucine were increased and T1/2 of strychnine and brucine were decreased after pretreatment with TGP. The toxicokinetics study showed that pretreatment with TGP could attenuate the absorption of strychnine and brucine, as well as accelerate their elimination. These results suggest that TGP possesses renoprotective effects.

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Simultaneous Determination of Strychnine, Brucine, Strychnine N-Oxide and Brucine N-Oxide in Crude and Fermented Nux Vomica by CZE with Ephedrine Hydrochloride as an Internal Standard

Cited by 8 publications

References 15 publications

Capillary electrophoresis for monitoring bioprocesses

Capillary electrophoresis for monitoring bioprocesses

Capillary electrophoresis of natural products: 2011–2012

Renoprotective Effects of Total Glucosides from Paeony against Nephrotoxicity Induced by Total Alkaloids from Semen Strychni

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