Simultaneous determination of soy isoflavone glycosides, daidzin and genistin by monoclonal antibody-based highly sensitive indirect competitive enzyme-linked immunosorbent assay

Medicago spp. as potential sources of bioactive isoflavones: Characterization according to phylogenetic and phenologic factors

“…Isoflavones are synthesized, accumulated and constitutively expressed as phytoalexins in response to pathogen attacks (Dakora and Phillips, 1996), contributing to the global strategies of plant defense mechanisms (Aloui et al, 2012) and modulation of the interaction of Fabaceae species with nitrogen-fixing bacteria in rhizobium-legume symbiosis (Antunes et al, 2006). Isoflavones are also known as having a wide range of beneficial biological activities in the human body (Mortensen et al, 2009), but their overconsumption have been suggested as potentially causing adverse effects (Setchell and Cassidy, 1999 values (Sakamoto et al, 2015). Nevertheless, the intake amounts of isoflavones are usually evaluated on the basis of aglycone forms because the isoflavone glycosides are converted into aglycones by intestinal flora in vivo and in vitro (Sakamoto et al, 2015).…”

Colloidal gold-based indirect competitive immunochromatographic assay for rapid detection of bioactive isoflavone glycosides daidzin and genistin in soy products

“…In this study, we developed an ICA for detection and determination of isoflavone glycosides DZ and GEN. This was accomplished by taking advantage of the interesting characteristics of DZ-MAb, which possesses high cross-reactivity against DZ (100%) and GEN (82%) (Sakamoto et al, 2015). The immunochromatographic strip test was based on an indirect competitive immunoassay using DZ-MAb as a detector antibody (Fig.…”

“…To evaluate the accuracy of the ICA system for quantitative analysis, the total concentrations of isoflavone glycosides DZ and GEN obtained from the ICA system were compared with those obtained from HPLC analysis, described previously (Sakamoto et al, 2015). As shown in Table 1, the two sets of data showed positive correlation, with the exception of a few data points, with a coefficient of determination (R 2 ) of 0.816.…”

“…In a previous study, a monoclonal antibody (MAb) against DZ (DZ-MAb) was prepared using the hybridoma method. Characterization of DZ-MAb revealed that it possessed a high cross-reactivity against DZ (100%) and GEN (82%), which enabled us to develop an indirect competitive ELISA (icELISA) method for the simultaneous determination of the sum of concentrations of soy isoflavone glycosides DZ and GEN in soy products (Sakamoto et al, 2015). In the present study, we developed an indirect competitive immunochromatographic assay (ICA) to simultaneously detect two major isoflavone glycosides (DZ and GEN) in soy products.…”

Bacterial Expression of a Single-Chain Variable Fragment (scFv) Antibody against Ganoderic Acid A: A Cost-Effective Approach for Quantitative Analysis Using the scFv-Based Enzyme-Linked Immunosorbent Assay

“…The subjects of target molecules for immunoassays have been expanded from large molecules (i.e., protein, peptide, and DNA) to small molecules (herbicides, natural products, and phytohormones). As such, many monoclonal antibodies (mAbs) against small molecules (haptens), such as amikacin, 1) carbamazepine, 2) aflatoxins, 3) and daidzin, 4,5) have been produced to develop mAb-based immunoassays for qualitative and quantitative analyses, which were shown to be simple and convenient analytical methods. Because the cost of recombinant antibodies (rAbs) produced using Escherichia coli is much lower than that of antibodies produced using hybridoma cells or other animal cell lines, 6) immunoassays using E. coli-derived antibodies represent an economical approach for analytical applications.…”