Colloidal gold-based indirect competitive immunochromatographic assay for rapid detection of bioactive isoflavone glycosides daidzin and genistin in soy products

Sakamoto, Seiichi; Yusakul, Gorawit; Pongkitwitoon, Benyakan; Tanaka, Hiroyuki; Morimoto, Satoshi

doi:10.1016/j.foodchem.2015.08.002

Cited by 26 publications

(14 citation statements)

References 21 publications

(21 reference statements)

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“…Some of them are cyromazine and melamine [48], aflatoxins [29,82], E. coli [58,108], Botulinum neurotoxin A and B [43], foot and mouth disease O, A, Asia 1 [148], levonorgesteral and metilprednosolone [143], daidzin and genisitin [145]. The dedection principle of multiplex LFA is the same as the one of analyte LFAs.…”

Section: Gold Labelled Barcode-style Lfasmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

456

315

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Section: Gold Labelled Barcode-style Lfasmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

456

315

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“…Sensitivity is a key performance factor for developments in analytical methods, especially when the target compound is highly toxic with a trace amount of exposure. When an antibody was applied for both ICA and icELISA, the ICA procedures can sometimes produce lower sensitivity than icELISA . Enhancing sensitivity is one of the challenges for ICA development.…”

Section: Resultsmentioning

confidence: 99%

“…The antibody was labelled with colloidal gold, which functions as a detection agent in the ICA. The preparation of colloidal gold‐conjugated MAb 4B8 followed previously reported methods . Initially, 2% ( w / v ) potassium carbonate (20 μL) was added to a colloidal gold suspension (1 mL).…”

Section: Methodsmentioning

confidence: 99%

“…When an antibody was applied for both ICA and icELISA, the ICA procedures can sometimes produce lower sensitivity than icELISA. [14][15][16] Enhancing sensitivity is one of the challenges for ICA development. Recently, external antibody formats have been developed for ICA, where the primary antibody reacts with target compounds before ICA testing.…”

Section: Sensitivitymentioning

confidence: 99%

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Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

Yusakul

Sakamoto

Chanpokapaiboon

et al. 2019

Phytochemical Analysis

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Introduction Monocrotaline (MCT), which is classified as a 1,2‐dehydropyrrolizidine alkaloid (DHPA), is a toxic compound that is mainly produced by Crotalaria spp. MCT contamination in cereals and herbs leads to hepatitis, gastroenteritis, pulmonary vasculitis and hypertension, and different types of cancer. The current analytical methods for MCT are complicated and expensive using liquid chromatography equipped with mass spectrometry detection. Objective The aim of this study was to develop a simple and sensitive preincubation format for an immunochromatographic assay (PI‐ICA) for MCT detection. Methodology We conducted the PI‐ICA via incubation of an MCT‐containing sample with an anti‐MCT monoclonal antibody conjugated with colloidal gold before strip dipping. We compared the PI‐ICA detection sensitivity with that of the conventional ICA (Conv‐ICA) format. Results The PI‐ICA was sensitive with a limit of detection (LOD) of 0.61 ng/mL, which is a 16‐fold improvement over the Conv‐ICA format. These results indicated that the PI‐ICA method exhibits high binding specificity for MCT and low cross‐reactivity towards retronecine, retrorsine, senecionine and heliotrine. Sample solutions from plants containing MCT and related DHPAs produced positive results via PI‐ICA analysis. Conclusions The proposed PI‐ICA system provides a highly sensitive method compared to Conv‐ICA. In addition, the developed PI‐ICA method is simple and highly effective for detecting MCT contamination.

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“…For qualitative and semi-quantitative detection, the colour of the test line can be evaluated directly by the naked eye. For quantitative assay, the OD of a test line can be measured with a test strip reader, and according to the regression equation from the standard curve, the level of the HES residue can be calculated [30–33]. …”

Section: Methodsmentioning

confidence: 99%

A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples

et al. 2018

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A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg−1, and the limit of detection value was 0.62 µg kg−1. For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91–4.64% and 4.24–5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.

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Colloidal gold-based indirect competitive immunochromatographic assay for rapid detection of bioactive isoflavone glycosides daidzin and genistin in soy products

Cited by 26 publications

References 21 publications

Lateral flow assays: Principles, designs and labels

Lateral flow assays: Principles, designs and labels

Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples

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