Simultaneous determination of selected flavonoids from different Cistus species by HPLC-PDA

Abstract

ABSTRACT:A rapid and simple high-performance liquid chromatography method with a Photo Diode Array (PDA) detector is developed for the simultaneous analysis of seven kaempferol, quercetin and myricetin derivatives along with an acylated flavonoid glycoside; trans-tiliroside from different Cistus species. In this study, the qualitative and quantitative analysis of the methanolic extracts of three Cistus species (C. creticus L., C. laurifolius L. and C. salviifolius L.) growing in Anatolia in terms of characterization of flavonoid compounds were performed by RP-HPLC for the first time. Trans-tiliroside, a mono-coumaroyl kaempferol glucoside was found to be the most abundant flavonoid in C. salviifolius (0.276±0.003 g/100 gextract) and C. creticus (0.253±0.001 g/100 gextract) while hyperin (0.149±0.004 g/100 gextract) and myricetin 3-O-β-galactopyranoside (0.139±0.006 g/100 gextract) were found to be the most intense flavonoids in C. laurifolius samples. The described HPLC method appears suitable for the determination of the Cistus flavonols and their glycosides and can be considered as an effective and alternative procedure for the identification and quantification of this important class of biologically active compounds.

UPLC-MS/MS Phytochemical Analysis of Two Croatian Cistus Species and Their Biological Activity

“…The phenolic profile of both extracts comprises phenolic acids, flavanols, flavonol glycosides and flavonones, which is in accordance with results obtained by previous Cistus studies. This is the first study of the phenolic composition in C. certicus and C. salvifolius aqueous extracts and the presence of phenolic compounds is in accordance with previous studies related to phenolic content in other Cistus species [ 11 , 42 , 53 , 54 , 55 , 56 ].…”

Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island

“…Quercetin-3- O -glucoside [(M–H) − at m/z 463] and quercetin-3- O -pentoside [(M–H) − at m/z 433] were identified based on their common MS 2 fragment ion at m/z 301, due to loss of hexosyl ( m/z 162) and pentosyl (132 u) moieties, respectively. Their presence as main constituents of different Cystus extracts (infusions, methanolic and hydroethanolic preparations) was previously reported by other authors ( 9 , 10 , 26 , 27 ), among other glycosylated forms of quercetin. Compound 5 , revealed a similar fragmentation pattern as compounds 3 and 4, being tentatively identified as quercetin- O -deoxyhexoside.…”

“…Moreover, anti-inflammatory potential of different Cistus sp . herbal preparations (aqueous, hydroethanolic or fractionated extracts) was proven through in vivo experiments (ex: paw edema model in rats), being also correlated with the presence of phenolic derivates ( 8 , 26 , 27 , 36 ). Hence, our findings are in line with previous reports on anti-inflammatory potential of Cistus sp .…”

High-performance Liquid Chromatography Analysis and Antimicrobial Activities of Libyan Cistus salviifolius Extract

“…All these results are supported by the study carried by Kada, et al [41]. According to the published paper, a mono-coumaroyl kaempferol glucoside, was found to be the most abundant flavonoid in C. salviifolius [42]. In another study, epigallocatechin derivatives were isolated from the air-dried herb of C. salviifolius [43].…”