High-performance Liquid Chromatography Analysis and Antimicrobial Activities of Libyan Cistus salviifolius Extract

Abstract

Aims: This research is focused on the in vitro evaluation of Cistus salviifolius L. antimicrobial properties and the determination of the contents of phenols and flavonoids.
 Study Design: This research is analytical study aimed to illustrate the antimicrobial properties and to analyze the methanolic extract contents of aerial parts of Cistus salviifolius L. by high-performance liquid chromatography techniques.
 Duration: The study was performed within six months in the Faculty of Pharmacy, Benghazy University.
 Methodology: Antimicrobial properties was tested against twelve organisms using Kirby-Bauer disk diffusion sensitivity test and the determination of the contents of phenols and flavonoids was evaluated by running high-performance liquid chromatography techniques.
 Results: The findings indicated that catechin is the most abundant flavonoid in C. salviifolius, while gallic acid was the major phenol in the methanolic extract of the plant. The results also revealed that the methanol extracts had a significant antimicrobial potential particularly against Bacillus subtilis and Escherichia coli with MIC (0.98 and 0.49) µg/ml respectively, furthermore the extract was effective against Aspergillus fumigatus with MIC 0.98 µg/ml. 
 Conclusion: C. salviifolius was highly rich with flavonoids and phenols and has a significant antimicrobial effect.