Simultaneous determination of selected eicosanoids by reversed-phase HPLC method using fluorescence detection and application to rat and human plasma, and rat heart and kidney samples

Aghazadeh‐Habashi, Ali; Asghar, Waheed; Jamali, Fakhreddin

doi:10.1016/j.jpba.2015.02.041

Cited by 14 publications

(6 citation statements)

References 28 publications

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“…To date, most simultaneous lipidomic assays developed by other researchers focused mainly on LC-MS/MS analysis of the samples from brain, liver, pancreas, kidney, spleen, heart, solid tumor, plasma, and spinal cord [36, 73–75], but little information on LM levels in skeletal muscles was reported. While caution should be exercised when comparing directly the lipidomic data obtained from different tissues, because profiles of PUFA-derived LMs are tissue-dependent, the importance of our new methodology and its immediate application to skeletal muscles should not understated.…”

Section: Resultsmentioning

confidence: 99%

Targeted quantification of lipid mediators in skeletal muscles using restricted access media-based trap-and-elute liquid chromatography-mass spectrometry

Wang

Bian

et al. 2017

Analytica Chimica Acta

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Lipid mediators (LMs) are a class of bioactive metabolites of the essential polyunsaturated fatty acids (PUFA), which are involved in many physiological processes. Their quantification in biological samples is critical for understanding their functions in lifestyle and chronic diseases, such as diabetes, as well allergies, cancers, and in aging processes. We developed a rapid, and sensitive LC-MS/MS method to quantify the concentrations of 14 lipid mediators of interest in mouse skeletal muscle tissue without time-consuming liquid-liquid or solid-phase extractions. A restricted-access media (RAM) based trap was used prior to LC-MS as cleanup process to prevent the analytical column from clogging and deterioration. The system enabled automatic removal of residual proteins and other biological interferences presented in the tissue extracts; the target analytes were retained in the trap and then eluted to an analytical column for separation. Matrix evaluation tests demonstrated that the use of the combined RAM trap and chromatographic separation efficiently eliminated the biological or chemical matrix interferences typically encountered in bioanalytical analysis. Using 14 LM standards and 12 corresponding deuterated compounds as internal standards, the five-point calibration curves, established over the concentration range of 0.031 to 320 ng mL−1, demonstrated good linearity of r2 > 0.9903 (0.9903 to 0.9983). The lower detection limits obtained were 0.016, 0.031, 0.062, and 0.31 ng mL−1 (0.5, 1, 2, and 10 pg on column), respectively, depending on the specific compounds. Good accuracy (87.1–114.5%) and precision (<13.4%) of the method were observed for low, medium, and high concentration quality control samples. The method was applied to measure the amount of 14 target LMs in mouse skeletal muscle tissues. All 14 analytes in this study were successfully detected and quantified in the gastrocnemius muscle samples, which provided crucial information for both age and gender-related aspects of LMs signaling in skeletal muscles previously unknown. This method could be applied to advance the understanding of skeletal muscle pathophysiology to study the role of LMs in health and disease. Furthermore, we will expand the application of this methodology to humans and other tissues/matrices in the near future.

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Section: Resultsmentioning

confidence: 99%

Targeted quantification of lipid mediators in skeletal muscles using restricted access media-based trap-and-elute liquid chromatography-mass spectrometry

Wang

Bian

et al. 2017

Analytica Chimica Acta

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“…Plasma and tissue samples were assayed for the concentrations of 8,9-, 11,12-, and 14,15-EET, 8,9-, 11,12-and 14,15-DHET, and 20-HETE following a procedure previously reported in our lab with some modifications. 25 Briefly, heart or kidney samples were thawed to 4 °C, and an aliquot (100 mg) was homogenized using a tissue homogenizer (Omni-TH Thomas Scientific, NJ) and centrifuged for 1 min at 15 000 rpm in a glass tube containing 200 μL methanol and 0.4 μL of 96% formic acid.…”

Section: Pharmacological Activity Of Diclofenac Ethyl Ester Traceable...mentioning

confidence: 99%

Reduced Heart Exposure of Diclofenac by Its Polymeric Micellar Formulation Normalizes CYP-Mediated Metabolism of Arachidonic Acid Imbalance in An Adjuvant Arthritis Rat Model: Implications in Reduced Cardiovascular Side Effects of Diclofenac by Nanodrug Delivery

et al. 2020

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In this study, we tested whether the extent of drug presence in the heart contributes to the elevated cardiovascular risk of nonsteroidal anti-inflammatory drugs (NSAIDs). A fluorescently tagged nanoformulation of an NSAID with high cardiovascular (CV) risk (diclofenac) was developed as diclofenac ethyl ester (DFEE) encapsulated in traceable (cyanine-5.5-labeled) polymeric micelles (DFEE-TM) based on methoxypoly(ethylene oxide)-block-poly(ε-caprolactone)(PEOb-PCL) (MW, 5000:3500 g/mol). Diclofenac pharmacokinetics and tissue distribution, as well as ex vivo near-infrared images of organs and whole bodies, were compared between healthy rats and rats with adjuvant arthritis (AA) following the administration of a single intravenous (iv) dose of DFEE-TM. Moreover, the biodistribution and antiarthritic activity of DFEE-TM were compared with those of free diclofenac (once-daily intraperitoneal, ip, 10 mg/kg for 7 days). The concentration ratios of cytochrome-P450-mediated cardiotoxic (20hydroxyeicosatetraenoic acid) over cardioprotective (epoxyeicosatrienoic acids) metabolites of arachidonic acid (ArA) in the heart, kidneys, and plasma were measured as markers of cardiotoxicity. The nanocarrier was found in the joints of AA, but not in those of healthy rats. Both free diclofenac and DFEE-TM comparably controlled AA. Diclofenac delivery via PEO-b-PCL micelles reduced the accumulation of diclofenac in the heart of AA rats. Despite similar antiarthritic activity, the polymeric micellar formulation showed a reduction in the ratio of cardiotoxic-over-cardioprotective eicosanoids of ArA in the heart and plasma of AA rats. The results showed the positive effect of diclofenac prodrug nanodelivery in changing the normal biodistribution of diclofenac away from the heart, leading to lowered diclofenac-induced biomarkers of cardiotoxicity in the heart and plasma of AA rats.

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“…Chavis et al used RP–HPLC with a UV detector (237nm) for the quantification of 5-HETE and 12-HETE in human plasma without derivatization [165]. Aghazadeh-Habashi et al used derivatization with NE-OTf for the simultaneous quantification of eicosanoids in human plasma and rat heart and kidney by the HPLC method using fluorescence detection [166]. Yue et al coupled the derivatization of eicosanoids with fluorescence detection using RP–HPLC to determine bioactive PGs, EETs, DiHETEs and HETEs in rat brain tissue, and achieved limits of detection (LODs) and LOQs ranging from 2–20 to 20–70 pg on the column, respectively [126].…”

Section: Sample Preparationmentioning

confidence: 99%

Modern Methods of Sample Preparation for the Analysis of Oxylipins in Biological Samples

et al. 2019

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Oxylipins are potent lipid mediators derived from polyunsaturated fatty acids, which play important roles in various biological processes. Being important regulators and/or markers of a wide range of normal and pathological processes, oxylipins are becoming a popular subject of research; however, the low stability and often very low concentration of oxylipins in samples are a significant challenge for authors and continuous improvement is required in both the extraction and analysis techniques. In recent years, the study of oxylipins has been directly related to the development of new technological platforms based on mass spectrometry (LC–MS/MS and gas chromatography–mass spectrometry (GC–MS)/MS), as well as the improvement in methods for the extraction of oxylipins from biological samples. In this review, we systematize and compare information on sample preparation procedures, including solid-phase extraction, liquid–liquid extraction from different biological tissues.

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Simultaneous determination of selected eicosanoids by reversed-phase HPLC method using fluorescence detection and application to rat and human plasma, and rat heart and kidney samples

Cited by 14 publications

References 28 publications

Targeted quantification of lipid mediators in skeletal muscles using restricted access media-based trap-and-elute liquid chromatography-mass spectrometry

Targeted quantification of lipid mediators in skeletal muscles using restricted access media-based trap-and-elute liquid chromatography-mass spectrometry

Reduced Heart Exposure of Diclofenac by Its Polymeric Micellar Formulation Normalizes CYP-Mediated Metabolism of Arachidonic Acid Imbalance in An Adjuvant Arthritis Rat Model: Implications in Reduced Cardiovascular Side Effects of Diclofenac by Nanodrug Delivery

Modern Methods of Sample Preparation for the Analysis of Oxylipins in Biological Samples

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