Simultaneous determination of rosuvastatin and atorvastatin in human serum using RP-HPLC/UV detection: Method development, validation and optimization of various experimental parameters

Shah, Yatrik M.; Iqbal, Zafar; Ahmad, Lateef; Khan, Abad; Khan, Muhammad Imran; Nazir, Shabnam; Nasir, Fazli

doi:10.1016/j.jchromb.2011.01.004

Cited by 70 publications

(48 citation statements)

References 49 publications

(48 reference statements)

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“…The influence of mobile phase pH on retention of atorvastatin and rosuvastatin has been investigated [90]. Since both of the analytes are acidic compounds, their retention on the reversed-phase column was expected to be pH dependant.…”

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

Nigović¹,

Mornar²,

Sertić³

2012

Chromatography - The Most Versatile Method of Chemical Analysis

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Section: High Performance Liquid Chromatographymentioning

confidence: 99%

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

Nigović¹,

Mornar²,

Sertić³

2012

Chromatography - The Most Versatile Method of Chemical Analysis

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“…The flask was allowed to reach room temperature, diluted with acetonitrile to volume, and mix. Any insoluble matter was allowed to settle, then 1.0 mL of the clear supernatant was transferred to a 100 mL volumetric flask; diluted with mobile phase to volume, and mix [19,20].…”

Section: Preparation Of Assaymentioning

confidence: 99%

High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study

Muneer¹,

Muhammad²,

Abrar³

2017

J Chromatogr Sep Tech

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“…For optimization of the chromatographic conditions, parameters of HPLC such as flow rate, mobile phase ratio, injection volume, temperature, and wavelength were nearly fixed in a set number of experiments [16]. By making changes in all the above parameters, the best one was selected at which elution response was significant, accurate and recorded a sharp symmetric peak as shown in Table 2.…”

Section: Optimized Rp-hplc Chromatographic Parametersmentioning

confidence: 99%

Development and Validation of a New Chromatographic Method for the Simultaneous Estimation of Serratiopeptidase, Aceclofenac and Paracetamol by RP-HPLC

U¹,

Afroze²,

Pradeepti³

et al. 2017

Pharm Anal Chem

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Background: Method development, validation is an important parameter for the simultaneous estimation of Serratiopeptidase (SERA), Aceclofenac (ACE) and Paracetamol (PCM) by RP-HPLC, is supposed to be a costly and tedious process. The present study revealed using cheap and cost effective solvent system for the simultaneous estimation of Serratiopeptidase (SERA), Aceclofenac (ACE) and Paracetamol (PCM).Objective: Development and validation of a new chromatographic method for the simultaneous estimation of Serratiopeptidase (SERA), Aceclofenac (ACE) and Paracetamol (PCM) by RP-HPLC of marketed formulations.Methods: Simultaneous determination of SERA, ACE and PCM were carried out by RP-HPLC at the wavelength 327 nm, flow rate 0.4 mL/min, and the mobile phase used was water: methanol in the ratio (50:50 v/v). Further validation parameters such as system suitability, linearity, accuracy, precision, specificity, LOD, LOQ and robustness were taken into account to carry out the validation of the method. Results:Absorbance maxima for the simultaneous determination were selected by the UV spectrophotometer and that was found to be 327 nm in methanol and water. During the process of RP-HPLC, the linearity was obtained in the concentration range of 2-10 µg/mL for SERA, 100-500 µg/mL for ACE and 20-100 µg/mL for PCM. Correlation coefficient (r) for SERA, ACE and PCM in methanol and water was found to be 0.9817, 0.991 and 0.9949 respectively. Conclusion:The RP-HPLC method was simple, accurate, precise, and rapid and can be used for the simultaneous determination of SERA, ACE and PCM in bulk and pharmaceutical dosage form. The method is also economical as RP-HPLC grade water methanol in a ratio of (50:50) was used to achieve all the validation parameters.

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Simultaneous determination of rosuvastatin and atorvastatin in human serum using RP-HPLC/UV detection: Method development, validation and optimization of various experimental parameters

Cited by 70 publications

References 49 publications

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study

Development and Validation of a New Chromatographic Method for the Simultaneous Estimation of Serratiopeptidase, Aceclofenac and Paracetamol by RP-HPLC

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